| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,083,219 | C→A | 23.9% | intergenic (‑25/‑258) | yefM ← / → hisL | antitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,083,219 | 0 | C | A | 23.9% | 63.8 / 5.6 | 25 | intergenic (‑25/‑258) | yefM/hisL | antitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base C (8/11); new base A (3/3); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
CTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCC > CP009273/2083007‑2083348 | ctcCGGGCGTTAGCGTGGGAGCGCGGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTANGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGt > 1:472752/1‑232 (MQ=255) aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAag > 2:621582/1‑244 (MQ=255) ctctCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCATTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGa < 1:621593/244‑1 (MQ=255) aCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAatga > 1:157263/1‑130 (MQ=255) gTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAg < 2:157260/130‑1 (MQ=255) ggCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTc > 2:409775/1‑100 (MQ=255) gCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCa < 2:433574/100‑1 (MQ=255) tGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTg < 1:409780/100‑1 (MQ=255) gCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAAt > 1:104341/1‑86 (MQ=255) ttCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGc > 1:354619/1‑98 (MQ=255) ttCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGttaa > 1:352611/1‑103 (MQ=255) tcatcaTTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAATTGTa < 2:104340/86‑1 (MQ=255) tcatTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGttaattaa < 2:352606/103‑1 (MQ=255) tcatTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGtta < 2:354614/98‑1 (MQ=255) aCAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCa < 2:460028/66‑1 (MQ=255) cAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCa < 1:367132/65‑1 (MQ=255) cTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCctct > 1:335530/1‑173 (MQ=255) gCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTAATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGc < 2:335525/173‑1 (MQ=255) cGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACtatttat > 2:612422/1‑64 (MQ=255) tGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTattaat < 1:612433/64‑1 (MQ=255) gTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAaga > 1:309040/1‑136 (MQ=255) gTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTa > 2:623996/1‑150 (MQ=255) tCAATCTCCTCTTTTGTACAGTTCATTGTACAAtgatga > 2:451319/1‑39 (MQ=255) aTCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGTTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGccc < 1:624007/150‑1 (MQ=255) aTCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGAc < 2:309035/136‑1 (MQ=255) tctcCTCTTTTGTACAGTTCATTGTACAATGATGAGCGt < 1:451325/39‑1 (MQ=255) | CTCCGGGCGTTAGCGGGGGAGCGCAGTAGATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCGCTTCGCTGTAGCTAATTGTACGCATGTCAATCTCCTCTTTTGTACAGTTCATTGTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCC > CP009273/2083007‑2083348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |