| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,468,869 | C→A | 17.1% | S136Y (TCT→TAT) | yfdR → | CPS‑53 (KpLE1) prophage; conserved protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,468,869 | 0 | C | A | 17.1% | 74.3 / 2.3 | 29 | S136Y (TCT→TAT) | yfdR | CPS‑53 (KpLE1) prophage; conserved protein |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base A (3/2); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01 | |||||||||||
TGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGCGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGC > CP009273/2468672‑2469072 | tgCAACAGAAGCGTATTGCTAGGACATCCACGCACCACTGAAACGCCTTCTTCCCGACTATAAACGGATGGAAGAAATGTTAGATCCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGAACACATAATGCAGGCAACCGAACGCCGTGATCTCGTGCTTGATGATGTCTATTTCTGCCCTGAACTGGAAGGCATCCCGGCAACCGATATGTTCAACGTGATTACACTGGTAGCggggngttcctttg > 2:248615/1‑264 (MQ=255) caccacTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTc > 1:46131/1‑170 (MQ=255) acTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGc < 2:46131/170‑1 (MQ=255) taAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACg > 2:590590/1‑185 (MQ=255) cGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGAt < 1:590601/185‑1 (MQ=255) agaaAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCttt < 1:564317/129‑1 (MQ=255) gaAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGTGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCgg > 1:164094/1‑164 (MQ=255) tACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGTGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCa < 2:164091/164‑1 (MQ=255) gAGCACGCCAGTGAAATATGCCGATCCCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTAt > 1:647780/1‑162 (MQ=255) ctcGCTAGTGAAATATGCCGATCTTATTATGTTGGCAACCGAACGCCGTGAGCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGTGTTTCTGCGCGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGc < 1:248619/273‑1 (MQ=255) aCGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGaa < 2:647769/162‑1 (MQ=255) gCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTc > 2:278606/1‑73 (MQ=255) gCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTATTTCTGGCCTGTACTGGAAGGCATCCCg > 1:254412/1‑97 (MQ=255) gTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGc < 1:278611/73‑1 (MQ=255) gTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTATTTCTGGCCTGTACTGGAAGGCATCCCGGCaa < 2:254408/97‑1 (MQ=255) gTGAAATATGCCGATCTCATTAGGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCAc < 1:194159/121‑1 (MQ=255) tGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGt > 2:138535/1‑124 (MQ=255) gATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATg < 1:138536/124‑1 (MQ=255) aTCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTATTTc > 2:70142/1‑56 (MQ=255) ctcATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCa > 2:342689/1‑73 (MQ=255) cATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTATTTCTGGc < 1:70142/56‑1 (MQ=255) cATTATGCGTGCAACCGATCGCCGTGATTTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTNGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCACGAATGTTTTCGAAATGGAAGGGTTTCTTCGCGGGAAATGTGTACCGCGAGATCTTAAAGTGAACGAAACaa < 1:495404/247‑1 (MQ=255) aTTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCt > 2:639888/1‑52 (MQ=255) ttATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATccc < 1:342694/73‑1 (MQ=255) tGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGcc < 1:639899/52‑1 (MQ=255) aaCGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCa > 1:273910/1‑54 (MQ=255) ccGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATccc < 2:273905/54‑1 (MQ=255) ggCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTgg > 1:247921/1‑75 (MQ=255) tgatgaTGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCAcc < 2:247917/75‑1 (MQ=255) | TGCAACAGAAGCGTATTGCCAGGACATCCCCGCACCACTGAAACGCCTTCTTCCTGACTATAAACGGATGGAAGAAAAAATAGATGCAGTAATCCGTGAGAAATACGGGTTACCTCCTGTTATGAGCACGCCAGTGAAATATGCCGATCTCATTATGCTGGCAACCGAACGCCGTGATCTCGGGCTTGATGATGGCTCTTTCTGGCCTGTACTGGAAGGCATCCCGGCAACAGAGATGTTCAACGTGATTCCACTGGCACCGGGTCATGCCTACGGGATGTTTATGGAACGTTTTAACGATTTATCGGAGTTACGCAAATGCGCATGAATGTTTTCGAAATGGAAGGGTTTCTTCGCGGGAAATGTGTACCGCGAGATCTGAAAGTGAACGAAACAAATGC > CP009273/2468672‑2469072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |