| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,706,007 | A→G | 10.5% | N96N (AAT→AAC) | yhjY ← | autotransporter beta‑domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,706,007 | 0 | A | G | 10.5% | 87.1 / 4.8 | 38 | N96N (AAT→AAC) | yhjY | autotransporter beta‑domain protein |
| Reads supporting (aligned to +/- strand): ref base A (16/18); new base G (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01 | |||||||||||
CAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCAGGTTGATGATCACTATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACC > CP009273/3705796‑3706241 | cAAATTGCTGGTTATAGCTGATTGGCGTCCAGGGTCGGGGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAATGTACTCACGCTGGCATGNCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCGTAGGAATTTCTTCCGCCAGATTGACTTcc < 1:476818/275‑1 (MQ=255) ggTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCATAAGGTACTCACGCTGGCATGCCATAACGGACCGGTCAGCGTGGTAGTACTGTCGCCAATCTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACg < 1:344918/275‑1 (MQ=255) ggTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAGTTCCAGCCcatactcat > 2:212830/1‑156 (MQ=255) cTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAGTTCCAGCCCATACTCATActg < 1:212834/156‑1 (MQ=255) aTCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTAACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCCGCGCCCTTTGGCTACTGTGAATACTTTGCGACAAAATATCGTTGTAATCAGGTTGATGATCAATATCCCAGGAATAACGATAGGTGGACAGTc > 1:169678/1‑275 (MQ=255) gTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAAt > 1:538069/1‑92 (MQ=255) gCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAGTTcc > 2:451758/1‑92 (MQ=255) ccAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCa < 2:538058/92‑1 (MQ=255) aaCTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAGTTCCAGcc < 1:451764/92‑1 (MQ=255) cGTTATACAACGAAGAGGTTGAGCCATCGTAATGTAATGCAACGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCaac > 2:29740/1‑85 (MQ=255) ataCATCGAGGTGGTTGGGCCATGGTAATGTAATGCCGCTACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCcat < 1:29740/87‑1 (MQ=255) cGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCcatactcatact > 2:195272/1‑90 (MQ=255) ggtggtTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGc > 2:292676/1‑117 (MQ=255) gtggtTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATActgctg < 1:195276/90‑1 (MQ=255) tggtTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAAtt < 2:498039/67‑1 (MQ=255) gtTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTcc > 1:341347/1‑67 (MQ=255) gtTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGa < 1:292681/117‑1 (MQ=255) tgCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGcc < 2:341342/67‑1 (MQ=255) ccATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTcc > 1:154655/1‑61 (MQ=255) cGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGcc < 2:154652/61‑1 (MQ=255) atgCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATActgc > 2:151886/1‑66 (MQ=255) tgCCTCGACCGGGCCGGTTGTAACCTGTACATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAgggg > 1:523306/1‑83 (MQ=255) ccGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTca > 1:111517/1‑69 (MQ=255) ccGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATc > 2:3333/1‑73 (MQ=255) ccGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCAGGTTGATGATCACTATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGAACTGATTAcc > 1:57373/1‑275 (MQ=255) cgcgACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGt < 1:151889/66‑1 (MQ=255) tcgACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTttc < 2:523295/82‑1 (MQ=255) gACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATc < 2:111516/69‑1 (MQ=255) gACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCcaca < 1:3333/73‑1 (MQ=255) aCCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCAGGTtgatga > 2:318014/1‑165 (MQ=255) ggCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCtt > 1:343473/1‑110 (MQ=255) ggCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCAGGTTGATGATCAc < 1:318019/165‑1 (MQ=255) ggTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGc < 2:343468/110‑1 (MQ=255) ttCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCa > 2:23082/1‑68 (MQ=255) ttCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGG‑TTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAACTATCGTTGTAATCAGGTTGATGATCACTa > 2:547876/1‑150 (MQ=255) tataAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCcaca < 1:303503/48‑1 (MQ=255) tataAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGAtt < 1:23082/68‑1 (MQ=255) tataAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGG‑TTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCGGGTTGATGATCACTATccc < 1:547887/150‑1 (MQ=255) | CAAATTGCTGGTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACCTGTTCATATAAAGGAAAATTCCAGCCCATACTCATACTGCTGGTCACATCCACAGGGGTTTCTTCCGCCAGATTGACTTCCAGTCCCAGCGCCCTTTGGCTACTTTGAATACGTTGCGACAAAATATCGTTGTAATCAGGTTGATGATCACTATCCCAGGTGTAACGCTCTGTGGAAAGTCCGGGTTGCGTATCAACGATATATTCTTGTTGCCAGGCGTAAGCTGTGTTGAGAAAAAAGGCACTGATTACC > CP009273/3705796‑3706241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |