| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,845,175 | G→T | 15.5% | Q324K (CAG→AAG) | ilvB ← | acetolactate synthase 2 large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,845,175 | 0 | G | T | 15.5% | 95.2 / 2.6 | 32 | Q324K (CAG→AAG) | ilvB | acetolactate synthase 2 large subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/12); new base T (3/2); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01 | |||||||||||
GACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACA > CP009273/3844968‑3845399 | gACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTAACCAGCTCTGCACGGTCGATATCGACATGAATGATTTTg > 1:59081/1‑275 (MQ=255) aCCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgctgct < 1:218757/227‑1 (MQ=255) ttGTCATCGACACAGGCGGCAACGGCGTTGANCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCTACATCAGCCTGAATCTCCACGTGCGGCTGCTTGATTTTACCCAg < 1:487476/215‑1 (MQ=255) aaCGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAANATCCGCCTGAATCGCCACGTGCGGCTGCTTGAttt > 1:570247/1‑188 (MQ=255) gCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTAATCAACATCCGCCTGAATCGCCACGTGCGGCAGCTTGATTTTACCCAGCTCNGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAAc > 1:511598/1‑229 (MQ=255) cTTAACGGATCGCACTCTTTCGGGATTGACCACGGAAACTCAAGCTGCAAATCCGCTACCAACTGGTGCCACTATGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCAAGGTCGNTATCGGCATGAATGATTTTGGAATTCGGACATAACTGCTCGGTTTTGACAAACACCCGGTCATCAAAAAGAGCACCGAGCACTATcaacaa > 1:482188/1‑275 (MQ=255) cTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGctc > 2:273020/1‑173 (MQ=255) aCGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCa < 1:273025/173‑1 (MQ=255) cGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTTAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAAcg > 1:285785/1‑201 (MQ=255) ggTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTTAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGtc > 1:244280/1‑181 (MQ=255) ccACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTTAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGtcatca < 2:244276/181‑1 (MQ=255) ccACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAAc > 1:79450/1‑151 (MQ=255) ccACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGtgc > 1:126378/1‑236 (MQ=255) tctGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCt < 2:79450/151‑1 (MQ=255) tctGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTgcg < 2:126377/236‑1 (MQ=255) ctGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCa > 1:477513/1‑104 (MQ=255) ctGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGaa > 1:509313/1‑145 (MQ=255) ctGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTATGCa > 2:147200/1‑104 (MQ=255) aCGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGt < 2:477505/104‑1 (MQ=255) aCGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGc < 2:509303/145‑1 (MQ=255) aCGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTATGCACGGt < 1:147203/104‑1 (MQ=255) gcgcTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTTAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCg > 2:310131/1‑140 (MQ=255) cgcTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTAGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAAAAAATACGACTCCTGCAAAATATAGTTGGTGCTGCGCACGACGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTATGGAAACa > 1:218739/1‑275 (MQ=255) cTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgc > 1:50/1‑65 (MQ=255) ttCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTTAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGttt < 1:310136/140‑1 (MQ=255) ccaccaGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGctc > 2:162939/1‑82 (MQ=255) caccaGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgctgct < 2:50/65‑1 (MQ=255) caGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCa < 1:162942/82‑1 (MQ=255) ccAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCa > 1:375941/1‑65 (MQ=255) gCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgctgc > 2:230697/1‑41 (MQ=255) cACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGt < 2:375936/65‑1 (MQ=255) gTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGa < 1:230701/41‑1 (MQ=255) | GACCAACGTCGGTGGTGATAATTGCATTGTCATCGACACAGGCGGCAACGGCGTTGATCAGGCCGTAATGGCTTAACGGATCGCACGCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACA > CP009273/3844968‑3845399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |