| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,244,145 | G→T | 13.7% | F750L (TTC→TTA) | plsB ← | glycerol‑3‑phosphate O‑acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,244,145 | 0 | G | T | 13.7% | 126.0 / 3.9 | 45 | F750L (TTC→TTA) | plsB | glycerol‑3‑phosphate O‑acyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base T (3/3); total (22/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01 | |||||||||||
ACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTAGCGTGCGAGAATGCGCCGGGTTGATATGCAACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAG > CP009273/4243887‑4244390 | acacaacacaTCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCNTCAAACGCACGGCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTg < 1:598737/274‑1 (MQ=255) cacaacacaTCGCAATTTATTTAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGACGCTATCGCTGATATACACTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTCGTCCAACAACTCCGGCGCGTTGAt > 2:609644/1‑275 (MQ=255) taaTTTGCAGATTATGGAAGGCCTGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATNAACTCCGCCAGTAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACg < 1:609655/274‑1 (MQ=255) cAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACGCAACAACCAGAAGGTGATAGCATAAAGTTGCa > 1:331024/1‑272 (MQ=255) cACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACg > 1:343506/1‑166 (MQ=255) cACGTCTGATGTAATCAACTCCGCCAGAAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATTACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCAAAGAACTCCGGCGCGTTGATGc > 1:132181/1‑155 (MQ=255) tCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACGgaga < 2:343501/166‑1 (MQ=255) tCTGATGTAATCAACTCCGCCAGCAACTGAAAAACCATCATCGTTTCAGCCGGTTCTGCATCGCCGCTATCGCTGATATACCCGTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTg < 2:132180/155‑1 (MQ=255) ccAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgc > 2:67043/1‑155 (MQ=255) ccAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCAACAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCCCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGAAGGGTTCCGGGCCCGCCTGGGGCCaacagccgg > 1:310909/1‑272 (MQ=255) acacacggctAAGCCTTGATCATTGCCGCCGTTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCCGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACGCCGGCGCGTTGAGGGCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTa < 2:124473/265‑1 (MQ=255) cAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGa < 1:67043/155‑1 (MQ=255) ttCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTg > 1:166951/1‑139 (MQ=255) gCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGc < 2:166948/139‑1 (MQ=255) ccgcTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACTGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTg > 2:135074/1‑162 (MQ=255) tATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACTGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCAc < 1:135075/162‑1 (MQ=255) aCCCTTCATCACGCAGTTGCAGCACCAGCGAACTTAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTAGCGTGCGAGAATGCGCCGGGTTGATATGCAACTCATCATCTTGCAGGGTAATCAGcccc < 1:225759/275‑1 (MQ=255) cTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGc > 2:231608/1‑145 (MQ=255) ttCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGc > 1:128557/1‑68 (MQ=255) ttCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGAc > 2:320463/1‑136 (MQ=255) catcaCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgc > 1:328651/1‑71 (MQ=255) catcaCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCCACAACCAGAAGGGGCTGGCATAACGTTGCAGCGTTTCCCGCGCGCCGGc > 2:621093/1‑196 (MQ=255) atcaCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTc < 1:231612/145‑1 (MQ=255) tcaCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTg < 2:128556/68‑1 (MQ=255) tcaCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGt < 1:320468/136‑1 (MQ=255) tcGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGTACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACAAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGcc < 1:621104/195‑1 (MQ=255) aCGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTgcagca < 2:328646/71‑1 (MQ=255) cGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTgc > 2:617174/1‑84 (MQ=255) gtgtCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGa < 1:617185/84‑1 (MQ=255) gtgtCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTAGCGTGCGAGAATGCGCCGGGTTg < 1:307057/224‑1 (MQ=255) tgtCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGtt > 2:486137/1‑51 (MQ=255) tgtCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTg > 2:603882/1‑88 (MQ=255) tgtCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGAt > 2:326144/1‑153 (MQ=255) aGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATg < 1:486145/51‑1 (MQ=255) aGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGc < 1:603893/88‑1 (MQ=255) aGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCa < 1:326149/153‑1 (MQ=255) aaaagaACGGAANCCCGCCTTGGAGAAGGACCCCGGCGCGTTGATGCCGTGCAGGCCGGGGAGACGTTTCGCGACGGTGGGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCAGAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTAGCGTGCGAGAATGCGCCGGGTTGATATGCAACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAg < 2:501065/270‑1 (MQ=255) agagAACTGAACACCGCCTTGTCGAAGATCTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCNGCTGTAGCGTGCGAGCATGCGCCGGGTTGATATGCAACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCCTCTCATTTg > 1:501074/1‑268 (MQ=255) agagAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCaacaac > 2:397390/1‑132 (MQ=255) agagAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCAt > 2:597410/1‑148 (MQ=255) agaACTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTtc > 1:92510/1‑88 (MQ=255) aaCTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGa < 1:397395/132‑1 (MQ=255) aaCTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACg < 1:597421/148‑1 (MQ=255) cTGAACACCGCCTTGTCGAATAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCa < 2:92510/88‑1 (MQ=255) tGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCgg > 1:31763/1‑159 (MQ=255) gaagaaCTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCcag < 2:31763/159‑1 (MQ=255) gaaCTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGAc > 1:178291/1‑88 (MQ=255) | ACACAACACATCGCAATTTATTGAATTTGCAGATTATGGAAGGCCGGATAAGGCGTTTTCGCCGCATCCGGCAATTCTCTCTGATTACCCTTCGCCCTGCGTCGCACTCTCAATCGTCAAACGCACGTCTGATGTAATCAACTCCGCCAGCAACTGATAAACCTTCATCGTTTCTGCCGGTTCGGCATCGCCGCTATCGCTGATATACCCTTCATCACGCAGTGTCAGCACCAGAGAACTGAACACCGCCTTGTCGAAGAACTCCGGCGCGTTGATGCCGTGCAGCACGGAGAGACGTTGCGCGACGGTGCGGCTCTCTTTCTCCAGCGTACCGCGGTTGATCGACGGGTTGGCACTCAACAACCAGAAGGTGATGGCATAACGTTGCAGCGTTTCGCGCGCGCCTGCGGCCAGCAGCTGTAGCGTGCGAGAATGCGCCGGGTTGATATGCAACTCATCATCTTGCAGGGTAATCAGCCCCTGACGTTGCATCTCATTTGCCAG > CP009273/4243887‑4244390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |