Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	3,915,133	Δ4 bp	100%	coding (276‑279/816 nt)	atpB ←	F0 sector of membrane‑bound ATP synthase, subunit a

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3915132	1 (0.050)	34 (1.810)	32/260	NT	97.1%	coding (280/816 nt)	atpB	F0 sector of membrane‑bound ATP synthase, subunit a
	?	CP009273	3915137 =	1 (0.050)					coding (275/816 nt)	atpB	F0 sector of membrane‑bound ATP synthase, subunit a

TTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTACATGTCTTTCACGCTACCATTAACAAAGCCGATCACCAGCTCAATCGCGGTCTGAAACTTACCTGGCACACCGCTGGTCGCCTTTTTGGCTACGCTACGGAATAAAACCAGGAACAACAGACCCAGCACCACCGAGAAGAACATGGAGTCAATATTGATTGTCCAGAAGGTGGCTGGGGGGTTTTGTGGATCCACCAGCGAGAATGTACGCAGGTCCAGC  >  CP009273/3914885‑3915352                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             ttCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATAAGGTTCATCAGGAATACCCAGACGAAGTACGTCAGGGCCAGCGGAGCAATAAGCTTGATTTTGACAGGGTAtcatacacgctcccattaaaaaagcc                                                                                                                                                                                                    >  2:384123‑M1/1‑248 (MQ=255)                                     tAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtcttt                                                                                                                                                                                                                         >  1:323255‑M1/1‑212 (MQ=255)                                             aaTCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGACTACCCAGCCGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctccatcgcggtctgaaacttacctggccacccgc                                                                                                                                                        >  1:103121‑M1/1‑204 (MQ=255)                                                                   gCCTTAGACATCGTTACGTTCACGTCAGCAGCCGGAACCACACGCAGTGCAGGCAGACCCATTACATGTTCAGCAATGTTCGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgcta                                                                                                                                 <  1:384129‑M1/275‑94 (MQ=255)                                                                                             cgagcacgagaacacacgaagagcagtcAGACCCAGTACATGGTCAGAAATGGACGGCAGCAGGTCGATAGGCAGGAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagac                                                                                                        <  2:103117‑M1/262‑119 (MQ=255)                                                                                                                   gcaggcagACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGTCAGTAAATCCATCAGGTTCATCTGGAATACACAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGATTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaa                                                                                                                       >  1:25093‑M1/1‑134 (MQ=255)                                                                                                                           aCCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccga                                                                                                                                                                                                  >  2:322742‑M1/1‑126 (MQ=255)                                                                                                                               aGTACATGTTCAGCAATGTACGGCAGTAGGTCGAGAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctgncacaccgcnggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaagaacatggagacaatatta                                                                     >  1:274539‑M1/1‑122 (MQ=255)                                                                                                                               aGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcac                                                                                                                                                                                              <  1:322748‑M1/154‑33 (MQ=255)                                                                                                                                                 tACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaact                                                                                                                                                                       >  2:349970‑M1/1‑104 (MQ=255)                                                                                                                                                    ggCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtcttt                                                                                                                                                                                                                         >  2:272213‑M1/1‑101 (MQ=255)                                                                                                                                                     gcagcaGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacc                                                                                                                                                                   <  1:349976‑M1/159‑60 (MQ=255)                                                                                                                                                        gcagGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacg                                                                                                                                                                                                                     <  1:272218‑M1/106‑10 (MQ=255)                                                                                                                                                         cagGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctacca                                                                                                                                                                                                               >  1:363603‑M1/1‑96 (MQ=255)                                                                                                                                                           ggTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaaaaacatggagtcaata                                                                        >  1:170631‑M1/1‑94 (MQ=255)                                                                                                                                                             tCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaa                                                                                                                                                                                                           <  2:363597‑M1/111‑20 (MQ=255)                                                                                                                                                               gATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaaaaacatggagtcaatattga                                                                    <  2:170626‑M1/244‑155 (MQ=255)                                                                                                                                                                aTAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAt                                                                                                                                                                                                                             >  1:91504‑M1/1‑89 (MQ=255)                                                                                                                                                                    gCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtcttt                                                                                                                                                                                                                         <  2:91500‑M1/90‑6 (MQ=255)                                                                                                                                                                                tCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaagaacatggagtcaatattgattg                                                                 >  2:116238‑M1/1‑73 (MQ=255)                                                                                                                                                                                  aGGTTCATCAGGAATACCCAGACTAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttg                                                                                                                                          >  1:154879‑M1/1‑71 (MQ=255)                                                                                                                                                                                    gTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaagaacatggagtcaatattgattgtcca                                                             <  1:116242‑M1/230‑162 (MQ=255)                                                                                                                                                                                      tcatcaGGCATACCCAGACTAAGAGCGTCAGAGGCAGCGGAGCAAGCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgacttattggcta                                                                                                                                      <  2:154874‑M1/155‑89 (MQ=255)                                                                                                                                                                                                  cccAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtcttt                                                                                                                                                                                                                         >  1:334079‑M1/1‑55 (MQ=255)                                                                                                                                                                                                   agagaaaaagatggtcAGGGCCAGGNGAGCAATCACTTTGCTTTTGCCTTGGTAtctttcacgctaccattaacaaagccgatcaccaggtcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgagaagaacatggagtcaatattgattgtccagaaggtggctggggggttttgtggatccaccagcgagaatgtacgcaggtccagctgaa  <  2:274534‑M1/267‑221 (MQ=255)                                                                                                                                                                                                      gACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacg                                                                                                                                                                                                                     <  2:334073‑M1/60‑10 (MQ=255)                                                                                                                                                                                                       aCGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaac                                                                                                               >  1:381839‑M1/1‑50 (MQ=255)                                                                                                                                                                                                           aGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaaca                                                                                                           <  2:381833‑M1/161‑116 (MQ=255)                                                                                                                                                                                                            gATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgc                                                                                                                                                                                                                    >  2:294744‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                gTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctacc                                                                                                                                                                                                                <  1:294750‑M1/55‑15 (MQ=255)                                                                                                                                                                                                                 tCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctg                                                                                                                                                                            >  2:85530‑M1/1‑40 (MQ=255)                                                                                                                                                                                                                     ggCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaac                                                                                                                                                                        <  1:85533‑M1/90‑55 (MQ=255)                                                                                                                                                                                                                           cGGAGCAATCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccga                                                                                                                                                                                                  >  2:278125‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                               gCAATCAGCTTGCTTTTGCCATGGTA‑‑‑‑TCTCTCATCCTACCATCAACAAAGCCGATCAc                                                                                                                                                                                          <  1:278130/58‑1 (MQ=255)                                                                                                                                                                                                                                   tCAGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgccttt                                                                                                                                             >  2:52101‑M1/1‑22 (MQ=255)                                                                                                                                                                                                                                     aGCTTGCTTTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccacc                                                                                             >  2:125378‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                                       cttgcttTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttgg                                                                                                                                         <  1:52104‑M1/103‑86 (MQ=255)                                                                                                                                                                                                                                         tgcttTTGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgctacggaataaaaccaggaacaacagacccagcaccaccgaga                                                                                         <  1:125382‑M1/149‑134 (MQ=255)                                                                                                                                                                                                                                            ttttGCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctac                                                                                                                                     >  1:8752‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                                gCCATGGTAtctttcacgctaccattaacaaagccgatcaccagctcaatcgcggtctgaaacttacctggcacaccgctggtcgcctttttggctacgcta                                                                                                                                 <  2:8752‑M1/102‑94 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                             TTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCCAGTACATGTTCAGCAATGTACGGCAGCAGGTCGATAGGCAGTAAATCCATCAGGTTCATCAGGAATACCCAGACGAAGATCGTCAGGGCCAGCGGAGCAATCAGCTTGCTTTTGCCATGGTACATGTCTTTCACGCTACCATTAACAAAGCCGATCACCAGCTCAATCGCGGTCTGAAACTTACCTGGCACACCGCTGGTCGCCTTTTTGGCTACGCTACGGAATAAAACCAGGAACAACAGACCCAGCACCACCGAGAAGAACATGGAGTCAATATTGATTGTCCAGAAGGTGGCTGGGGGGTTTTGTGGATCCACCAGCGAGAATGTACGCAGGTCCAGC  >  CP009273/3914885‑3915352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.