| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 57,344 | T→A | 28.6% | intergenic (‑235/‑20) | lptD ← / → djlA | LPS assembly OM complex LptDE, beta‑barrel component/membrane‑anchored DnaK co‑chaperone, DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 57,344 | 0 | T | A | 28.6% | 29.4 / 12.7 | 21 | intergenic (‑235/‑20) | lptD/djlA | LPS assembly OM complex LptDE, beta‑barrel component/membrane‑anchored DnaK co‑chaperone, DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (7/8); new base A (3/3); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGTAGTGTTAGGCCTGTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAAATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGTTTTTTGCCACCACTTTTGAA > CP009273/57117‑57543 | ttttATTGTTCCATCATCGGTAACGTTGCGCGTGACGAACGGTCAGATACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAAAGGTAAAAGCACCTTTATATTGTGGGAGATAGACCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcg < 1:239309/275‑1 (MQ=255) gTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCTATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGAttt > 2:167647/1‑152 (MQ=255) gAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCTATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTgggg < 1:167652/152‑1 (MQ=255) aGGCACGGCATTGAATGACAGGTATGATAGTGCAAATTATAGGCGATGTTCCACAATTGACCGCAGCTGGAAAACGGTAAAAGCACCTGTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGAAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGACTTAATGATGGGCGGGGGCTTTTGGGGCGGAGTGTTAGGCCAGTTAATTGGCCATATGGTGGATAAAGCCGGTAGCCGTAAAAtttgggggttgg > 2:338780/1‑260 (MQ=255) ggCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGGAata > 1:175807/1‑131 (MQ=255) ggCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaaa > 1:372070/1‑150 (MQ=255) ttGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGGAATATATg < 2:175802/131‑1 (MQ=255) ttGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa < 2:372064/150‑1 (MQ=255) aTGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTTGcgtggccgtg > 2:322533/1‑151 (MQ=255) tAATGCAAATTATAGGCGATGTCCCAAAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGACTATATGCAGTATTGGGGAAAAATCCTTGGCGTGGCCGTGGCCt < 1:322539/151‑1 (MQ=255) cgttGTCCCACAATTGACCTCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGAGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCNTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGTAGTGTTAGGCCTGTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAAATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGTTTTTTGCCACCACTTTTGaa < 1:338786/272‑1 (MQ=255) aTGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGAttt > 2:17344/1‑84 (MQ=255) gTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTgg > 1:191706/1‑154 (MQ=255) cccACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTgggg < 1:17345/84‑1 (MQ=255) cacaATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCg < 2:191701/154‑1 (MQ=255) aGCCGGAAAACGGTAGAAGCACCGGTATACTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTggg > 1:283724/1‑138 (MQ=255) ggAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGt < 2:283719/138‑1 (MQ=255) tAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTggg > 2:218145/1‑55 (MQ=255) aGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAat < 1:218150/55‑1 (MQ=255) tgGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggcc > 1:269748/1‑75 (MQ=255) agaTAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccgtgg < 2:269743/75‑1 (MQ=255) | TTTTATTGTTCCATCATCGGTAACGTTGCGCGTGACAAACGGTCAGAGACTAACGTACTCGTCATCTCTACGCTAGTGTTAATCCTGTCCGAATAGCGTCAGTGGTGTTAGGCACGGCATTGAATGACAGGTATGATAATGCAAATTATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGTAGTGTTAGGCCTGTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAAATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGTTTTTTGCCACCACTTTTGAA > CP009273/57117‑57543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |