Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,390,856	C→A	12.2%	G100V (GGG→GTG)	rng ←	ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,390,856	0	C	A	12.2%	89.9 / 2.9	34	G100V (GGG→GTG)	rng	ribonuclease G
Reads supporting (aligned to +/- strand):  ref base C (15/14);  new base A (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

CCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATA  >  CP009273/3390622‑3391103                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        ccAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCACCAGAGCATGCCCCTGACGAACCAGGTACGAGACGTcgctgtctattaga                                                                                                                                                                                                                   >  2:109927/1‑262 (MQ=255)     ccccTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGtt                                                                                                                                                                                                                                          <  1:109931/246‑1 (MQ=255)                     aCGGTTGATATACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCACCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCca                                                                                                                                                                                             <  1:157606/275‑1 (MQ=255)                           gatAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGAACTTGCACCTGACGAACCAGTTCCGAGACGTCGCGCCCCGTGAATTTGTTTTGTTCGTCACCCNCcaccacgt                                                                                                                                                                                       >  2:157601/1‑270 (MQ=255)                               aaCCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCtt                                                                                                                                                                                                              <  1:43022/248‑1 (MQ=255)                                     cccTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGATCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTAGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACAAATCCGGTGTGCg                                                                                                                                                                              >  1:375786/1‑274 (MQ=255)                                                         tGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTc                                                                                                                                                                                                                                         >  1:38086/1‑195 (MQ=255)                                                             aCCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCgag                                                                                                                                                                                                                                     <  2:38085/195‑1 (MQ=255)                                                                                         aTTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCACCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGtgtg                                                                                                                                                                                >  1:54849/1‑220 (MQ=255)                                                                                             gCTTTCAATACGTTGGGAAACCCCAACGGGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCACCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGc                                                                                                                                                                            <  2:54846/220‑1 (MQ=255)                                                                                                        gTTGGGAAACCCCAACGTGAGAAGCCCCTGACATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCCCGCACCTTTAGTGCCCAGCGGAGCTTTCACCACATCCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGAAGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGCTAGACGGCATGATTTAGGATTCATGAANGAACGCGGCTTTACCCAGCCCGATGTGTACAGAAgccacccccc                                                                                                            >  2:260008/1‑269 (MQ=255)                                                                                                                                ccccTGGCAGAAACACCAGAGAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGNACCATCAGATCTTGCCCCTGACGAACCAGTGCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGa                                                                                  <  1:290818/275‑1 (MQ=255)                                                                                                                                           aaCACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGAc                                                                                                                                                                                                                                                   >  2:176582/1‑103 (MQ=255)                                                                                                                                              aacAGTTAGAGGGAAGGGAGCGGATTTTCTGTGGTCAGGCGCGCCCCTTTAGTGCCAAGCGGATCTTTCCCCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGata                                                                      <  2:282987/271‑1 (MQ=255)                                                                                                                                               ccAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAAc                                                                                                                                                                                                                                               <  1:176587/103‑1 (MQ=255)                                                                                                                                                     aGCGAGAAGGGAGCGGGATATTGCTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAANAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCATTTTa                                                                          >  1:282992/1‑262 (MQ=255)                                                                                                                                                       cGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGAc                                                                                                                                                                                                                                                   >  2:390546/1‑91 (MQ=255)                                                                                                                                                           aaGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCTCCTGACGAAc                                                                                                                                                                                                                                               <  1:390552/91‑1 (MQ=255)                                                                                                                                                                    tGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCCCCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTc                                                                                                                                                                                                                                >  1:202500/1‑97 (MQ=255)                                                                                                                                                                        atCTGTGGTCAGACGCGCACCTTTAGTGCCAAGCTGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCgcgc                                                                                                                                                                                                                            <  2:202495/97‑1 (MQ=255)                                                                                                                                                                          cgttggtCAGGCGCTCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACg                                         <  1:260013/271‑1 (MQ=255)                                                                                                                                                                                 gaGGCGCTCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGTGCTTGCCCCTGACGAACCATTTCCGGGCTGTCGCGCACCGTGAATTTCTTTTGTTCTTCACCCGCCACACCTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACTCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAatat                                 <  1:380709/274‑1 (MQ=255)                                                                                                                                                                                                      cacctcctCTTTCACCCCCTGCCCCATCAGATCCTGCCCCTGACCCCCCCGTACCGAGTTGTCGCGCCCCTTTAATTGCTTTTGTTCTTCCCCCGCCGCACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGc            <  2:2813/268‑1 (MQ=255)                                                                                                                                                                                                      aaGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGAc                                                                                                                                                                                                                                                   >  1:301661/1‑44 (MQ=255)                                                                                                                                                                                                      aaGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTTCGGCATGATGTCGGATGCATgaaga                                                                                                                                                       >  2:314113/1‑136 (MQ=255)                                                                                                                                                                                                      aaGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCNGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATAcag             >  1:314922/1‑272 (MQ=255)                                                                                                                                                                                                          ggATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAAc                                                                                                                                                                                                                                               <  2:301655/44‑1 (MQ=255)                                                                                                                                                                                                          ggATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcg                                                                                                                                                   <  1:314119/136‑1 (MQ=255)                                                                                                                                                                                                                 tCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCCCACGTTCAATATGAATTTCCTGAAGAATACCGCCATCAAtata  >  1:2813/1‑274 (MQ=255)                                                                                                                                                                                                                        cTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATgaaga                                                                                                                                                       >  1:189752/1‑118 (MQ=255)                                                                                                                                                                                                                            aCCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAAcg                                                                                                                                                   <  2:189747/118‑1 (MQ=255)                                                                                                                                                                                                                               aTCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGcc                                                                                                                                    >  2:302261/1‑130 (MQ=255)                                                                                                                                                                                                                                   gATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGTCTTTATCCAGCCCAat                                                                                                                                <  1:302267/130‑1 (MQ=255)                                                                                                                                                                                                                                        tGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACaa                                                                                                                        >  2:338453/1‑133 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        CCAACCCCTTCCGCTGCGGTACGGATGATAAACCCGCCCTGCTCGTCGCAATACTCTGCGACCACTTTTTTCAGGCGTTCACGTTCTGATTCGCTTTCAATACGTTGGGAAACCCCAACGTGAGAAGCCCCTGGCATAAACACCAGATAGCGAGAAGGGAGCGTGATATCGGTGGTCAGGCGCGCACCTTTAGTGCCAAGCGGATCTTTCACCACCTGCACCATCAGATCTTGCCCCTGACGAACCAGTTCCGAGATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATA  >  CP009273/3390622‑3391103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑