| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,840,764 | C→A | 23.9% | W407C (TGG→TGT) | uhpC ← | membrane protein regulates uhpT expression |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,840,764 | 0 | C | A | 23.9% | 30.8 / 3.0 | 16 | W407C (TGG→TGT) | uhpC | membrane protein regulates uhpT expression |
| Reads supporting (aligned to +/- strand): ref base C (8/4); new base A (2/2); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
CGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCAT > CP009273/3840553‑3841024 | cGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGAAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGATTACTTTCTCCAGCGGCAAACCAGCAAGCGACGCCACCAGATAAGCaactgt > 1:237177/1‑270 (MQ=255) gCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCACCCAGCAAGCGACGCCCCCAGATAAGCCAACAAGCCGACAAACCCCTACGCCGCCCCTGCCGCCTCTTTGTGGGAaccca > 1:198634/1‑271 (MQ=255) ggCAAAACCTGGAAAATTTCTTAGTTTCGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGAGGCGATAGAGATAACCACAAAAAATCAGCTCCAGTGCCAGGTATCGGGTAGTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCGGATAAGCAAAAAAGCCGACAAACCCCGTCGCCGCACCACCCCCCGCCTTGCGGGACAACTCTgccgc > 1:242811/1‑274 (MQ=255) ttCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCa > 1:321423/1‑151 (MQ=255) cgcgGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACa < 2:321417/151‑1 (MQ=255) ttCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCaa > 1:87801/1‑112 (MQ=255) aaaaGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGa < 2:87798/112‑1 (MQ=255) gTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGACAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgc > 1:373421/1‑151 (MQ=255) gTAACAGCAGTGCGGAAATCCCGGCGGCAATAGAGATAACCACAAAAAATCCGCTCCAGTGACAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgc > 1:285842/1‑151 (MQ=255) cagcagTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAAGCCGCTCCAGTGACAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTgccgcc < 2:373415/151‑1 (MQ=255) cGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACg > 2:390/1‑93 (MQ=255) cGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTgccgccgcc > 1:215027/1‑170 (MQ=255) aaTCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGcccc < 1:390/93‑1 (MQ=255) aaaaTCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATGAACATCNGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTc > 1:341497/1‑275 (MQ=255) aaaaagCTCCAGTGACAGGTACCGAGTACTGTCGCCAGCGCCCAACCAGCAAGGGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGCCGCCGCCCCGGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCCATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCAt < 2:341491/270‑1 (MQ=255) ngTGCCAGGTATAGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACCAAAAGACCAATGGCGAAGAAGCAGGTTGc < 1:290524/183‑1 (MQ=255) | CGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAGGTATCGAGTACTTTCGCCAGCGGCCAACCAGCAAGCGACGCCCCCAGATAAGCAAACAAGCCGACAAACCCCGTCGCCGCCCCTGCCGCCTCTTTGTGGGAACACTCTGCCGCCGCCATACCGATTAACATCTGTGGGCCAAAGACAAAAAAACCAATGGTGAAGAAGCAGGTTGCCTGCATCACGTAGCTGGCAAATGGCATCAGCCACAGGGAGCCGACTGAAAGCAAAATTCCGGCGGCGAAAATCAAATTCAT > CP009273/3840553‑3841024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |