| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,265,386 | G→T | 18.3% | intergenic (‑417/‑13) | yjcB ← / → yjcC | putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,265,386 | 0 | G | T | 18.3% | 48.6 / 4.1 | 22 | intergenic (‑417/‑13) | yjcB/yjcC | putative inner membrane protein/putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base T (2/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
TGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAGCGATGCAGCTGAACGCTATCAGGGGCAAGTTTGCACTC > CP009273/4265145‑4265627 | tGACTGTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATCTCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACTGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACTTTGCAATGTCTGGTTATAAACAAGCAGGAAATTGATATCAATGAGTCATCGTGCCCGaaac > 2:108549/1‑275 (MQ=255) tgtgGTTTTTCAGTTGCATGTAGGGCATATTTTCCCTCCGCCTCTTCATTCCTGTATGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCAtt < 1:172922/275‑1 (MQ=255) ggAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGTAAATTGATAGCaa > 1:348440/1‑175 (MQ=255) attaattTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGTAAATTGATAGCAATGAg < 2:348434/175‑1 (MQ=255) ttttCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTAGCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGTATTACCTTCCTTTGGCCAACATCAGAAGTGCATAATCAGCTcctcc > 2:347003/1‑272 (MQ=255) cATCCAGGTGACGATTAGAAAGGTATTAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGAATTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAAGNGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGaataat < 1:347009/261‑1 (MQ=255) gCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGc > 1:24412/1‑275 (MQ=255) aaGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTATCTTTCCCATCATTCTTTCGCTATGGATTTCCTTCCTTTGTGCAAAATCAgca > 2:32849/1‑188 (MQ=255) aGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTATCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTTGGCAAAATCAGAAGTGAATAATCAGc > 2:23396/1‑202 (MQ=255) tttATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGa < 1:32850/190‑1 (MQ=255) ttATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCg < 1:23397/202‑1 (MQ=255) aTCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAGCGATGCAGCTGa < 1:108553/275‑1 (MQ=255) tCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCttttt > 2:70107/1‑114 (MQ=255) tACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGtt < 1:70110/114‑1 (MQ=255) ccAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGTAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTtc > 1:19467/1‑112 (MQ=255) ggTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGTAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCtt < 2:19466/112‑1 (MQ=255) tttCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAg > 1:159703/1‑234 (MQ=255) aCCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAg < 2:159698/234‑1 (MQ=255) ccGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAg > 1:61604/1‑218 (MQ=255) gTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTtc > 1:39627/1‑85 (MQ=255) gTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAg < 2:61601/218‑1 (MQ=255) aaaCAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCtt < 2:39626/85‑1 (MQ=255) gcaggAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAGCGATGCAGCTGAACGCTATCAGGGGCAAGTTTGc > 1:329202/1‑242 (MQ=255) gAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAGCGATGCAGCTGAACGCTATCAGGGGCAAGTTTGCACTc < 2:329196/242‑1 (MQ=255) | TGACTTTTATTGCTGTACAGATTATGTGGTTTTTCAGTGGCATTAAGGGCATATCTTCCCGCCGCCTCTGCATTCCTGTAGGAAATTAATTTTGAATATCAATGAATTATTTTCATCCAGGTGACGATTAGAAAGGTATCAATTTCAAATCAGGCAAAAGTGCTATTTATACCGTAAGATTTATCTAAAGACGTCGGTACCCAGGGTTTTCACCTTGCAATGGCCGGGTATAAACAGGCAGGAAATTGATAGCAATGAGTCATCGTGCACGACACCAATTACTGGCGTTGCCGGGCATTATCTTTTTAGTTCTCTTTCCCATCATTCTTTCGCTATGGATTGCCTTCCTTTGGGCAAAATCAGAAGTGAATAATCAGCTCCGAACCTTTGCTCAACTGGCACTGGATAAATCCGAGCTGGTCATTCGCCAGGCAGATTTAGTGAGCGATGCAGCTGAACGCTATCAGGGGCAAGTTTGCACTC > CP009273/4265145‑4265627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |