Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 879,076 G→T 100% intergenic (‑232/‑53) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273879,0760GT100.0% 90.0 / NA 29intergenic (‑232/‑53)ybjG/mdfAundecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/14);  total (15/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCG  >  CP009273/878815‑879317
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
gaATAGAGAGAGATTCAAATTTTCCAGTATAACTTTCCGACGCAAAGTGATGAAAAGGGAGCCAAGACAGGCAAGTCGTTGAGAATAAAGNGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTGAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCTTGGTTTCAGCTAGAAAATTCGCTTTCTCTTTAGCTGCGCtt                                                                                                                                                                                                                                       <  1:308098/274‑1 (MQ=255)
                                                                                                         gTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTATATAAAACTTAAAGAAAGCGTAGCTATCCTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGATTTTATTAAACTCTGCGCGCTTCTTATTGGCGAAGAAATTGAATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTGCTTTTtccgcctctcttt                                                                                                                             >  2:54323/1‑265 (MQ=255)
                                                                                                             aGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCACTGTCTGGTGc                                                                                                                         >  1:142642/1‑275 (MQ=255)
                                                                                                                                 cTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAActc                                                                                                                                                                                                                            >  2:47571/1‑156 (MQ=255)
                                                                                                                                     aGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTgcg                                                                                                                                                                                                                        <  1:47572/156‑1 (MQ=255)
                                                                                                                                         gccgggtgaCGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAAGTCGCTTTCTATTTAGCGGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCTAAGAAATTTCATGCAAAATAAATTAGCTTCCTGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCaa                                                                                             <  2:142637/267‑1 (MQ=255)
                                                                                                                                                                               aataaaACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGattattatt                                                                                                                                                                                                             >  1:101758/1‑125 (MQ=255)
                                                                                                                                                                                   aaaCTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCg                                                                                                                                                                                                         <  2:101754/125‑1 (MQ=255)
                                                                                                                                                                                               agCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTAc                                                                                                                                              >  2:91033/1‑172 (MQ=255)
                                                                                                                                                                                                   tAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACtttt                                                                                                                                          <  1:91037/172‑1 (MQ=255)
                                                                                                                                                                                                           cTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCgtgg                                                             >  2:20900/1‑241 (MQ=255)
                                                                                                                                                                                                              gTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTcc                                                                                                                                        >  2:223564/1‑163 (MQ=255)
                                                                                                                                                                                                              gTAATAATGTAAGAATGTGCTTAACCGTGGTTTAAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAActc                                                                                                                                                                                                                            >  2:72443/1‑79 (MQ=255)
                                                                                                                                                                                                               taataaTGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGa                                                         <  1:20901/241‑1 (MQ=255)
                                                                                                                                                                                                                  taatGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCctct                                                                                                                                    <  1:223569/163‑1 (MQ=255)
                                                                                                                                                                                                                  taatGTAAGAATGTGCTTAACCGTGGTTTAAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTgcg                                                                                                                                                                                                                        <  1:72446/79‑1 (MQ=255)
                                                                                                                                                                                                                    atGTAAGAATGTTCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGNGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCGGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCGCGCGTCCATTGATGGGGTTCCTACTTCGa                   <  1:281975/274‑1 (MQ=255)
                                                                                                                                                                                                                     tGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGa                                                                                                                                                           >  2:318231/1‑137 (MQ=255)
                                                                                                                                                                                                                     tGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCGGGTGCTTTACGAATTTTCAACCTATATAGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGATCATTGATTGGATTCCTACTTctg                   >  2:281970/1‑271 (MQ=255)
                                                                                                                                                                                                                         aGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTc                                                                                                                                                       <  1:318237/137‑1 (MQ=255)
                                                                                                                                                                                                                             tgtgCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATtg                                                                                                                                                                                                >  2:233429/1‑92 (MQ=255)
                                                                                                                                                                                                                               tgCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGcc                                                                 >  1:367216/1‑217 (MQ=255)
                                                                                                                                                                                                                                 cTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATtgcatg                                                                                                                                                                                            <  1:233434/92‑1 (MQ=255)
                                                                                                                                                                                                                                   tAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCgtgg                                                             <  2:367210/217‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGAAACGATATGATTGAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATGGATTGGGTTACAACTTCGATGAACGCGTATCTggcg  >  1:201937/1‑275 (MQ=255)
                                                                                                                                                                                                                                     aCCGTGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGattatta                                                                                                                                                                                                               >  2:311308/1‑69 (MQ=255)
                                                                                                                                                                                                                                         tGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTgg                                                                                                                                                                                                           <  1:311314/69‑1 (MQ=255)
                                                                                                                                                                                                                                         tGGTTTCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTatat                                                                                                   >  2:126269/1‑173 (MQ=255)
                                                                                                                                                                                                                                             ttCAGCTACAAAATTCGCTTTCTCTTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGc                                                                                               <  1:126273/173‑1 (MQ=255)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
GAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTACGGTGGGTGATTGTAACGGGCATAGGTTAAATAAAACTTAAAGAAAGCGTAGCTATACTCGTAATAATGTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATTATTATTGGCGAAGAAATTGCATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATTTTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCG  >  CP009273/878815‑879317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑