| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,663,222 | G→T | 21.0% | L210I (CTC→ATC) | ynfL ← | LysR family putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,663,222 | 0 | G | T | 21.0% | 45.3 / 4.5 | 19 | L210I (CTC→ATC) | ynfL | LysR family putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (7/8); new base T (2/2); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
TTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCA > CP009273/1662990‑1663407 | ttACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCaaaaaa > 1:139211/1‑275 (MQ=255) tCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGATAATATCGTCATACAGCCCTGTCCCGACGTGCGGATc > 1:260834/1‑193 (MQ=255) cACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGATAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCaaaa < 2:260829/193‑1 (MQ=255) cgcATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAggg > 2:236068/1‑197 (MQ=255) tttCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCt < 1:236073/197‑1 (MQ=255) cgtaaGCAGAACAACTTTAAATGACGCAGGCAAAATTGACACACCCAGACCGGCGGAAACCAGGCCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGa < 2:139206/271‑1 (MQ=255) ggCAAAATTGAAACACCCTGACCGGCTGAAACCAGTCTGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGAGTGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGAGGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCgg < 1:135274/275‑1 (MQ=255) aaCACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAata > 1:274067/1‑103 (MQ=255) aaCACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCCCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAACGGGTTCTTTCGCCAGTTCAGCCAGCGTTACAATCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCCTAAGCTGTTCATGGACGATGACTGCGTGTTCAAGCGCCTCCGGTAACGCTgtgt > 1:225112/1‑275 (MQ=255) cccAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGt < 2:274062/103‑1 (MQ=255) aCCGGCGGAAACCAGACCGTTGATGGTCATTGCCTCGCCGACCTCCTGAGTTATGACGGGCGTCAAATGGTAACGTGGCATCAGACCCAGAATATCGACATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACgcagca < 1:205011/275‑1 (MQ=255) aCCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCgg > 1:77060/1‑209 (MQ=255) gACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCa < 2:77057/209‑1 (MQ=255) tGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGATAATATCGTCATAc > 1:340425/1‑57 (MQ=255) aGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGt > 2:95391/1‑50 (MQ=255) tgatgaCGGGCGTCAAATGGTAACGTCGCATCAGCCCGATAATATCGTCATACAGcc < 2:340419/57‑1 (MQ=255) atgaCGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATa < 1:95395/50‑1 (MQ=255) gggCGTCAAATGGTAACGTCGCATCAGCCCGAGaa < 1:63771/35‑1 (MQ=255) cGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACa > 1:111802/1‑50 (MQ=255) | TTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGATGGTCATTGCCTCGCCCACCTCCTGAGTGATGACGGGCGTCAAATGGTAACGTCGCATCAGCCCGAGAATATCGTCATACAGCCCTGTCCCGACGTGCGGATCAAAAAAGACAAAGGGTTCTTTCGCCAGTTCAGCCAGCGTTACATTCGGGTTATTTGCCAGGGGATGATCGTGCGGGATCATCGCCATAAGCGGTTCATGGACGATGACTGCGTGTTCAAGCGACTCCGGTAACGCTGTGTTACGCAGCA > CP009273/1662990‑1663407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |