Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,423,519 T→C 7.4% D108G (GAT→GGT)  qorB ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,423,5190TC 7.4% 158.9 / 4.5 54D108G (GAT→GGT) qorBNAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

GGCACTATCGCCCGCCAGTTCGTAAACCTTGCCTTCGTGACCGGCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCATCGCCGTAGTCAGCCTGACGCACGGTAATGCCTTGTGCTGCCAGGGCTTGG  >  CP009273/4423245‑4423728
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                 
ggCACTATCGCCCGCCAGTTCGTAAACCTTGCCTTCGTGACCGTCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTCTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTAt                                                                                                                                                                                                                   <  1:153625/275‑1 (MQ=255)
    caagtgcccgccGGGTCGTAAACCTGGCCTGCGGGACCGGCTTCGCTAATCACGCGTGCCGCCGCTGCCGCATAATCTGCCCGCGGTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAAGACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGc                                                                                                                                                                                                               <  2:605886/270‑1 (MQ=255)
       cggcccgccNTTCCGGAACCCGTGCCTCCGTGACCGGCTTCGCTAATCACGCCTGCCGCAGCTGCCGCCTAATCGGCCCGCGTGGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGGGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATg                                                                                                                                                                                                            <  2:277560/273‑1 (MQ=255)
                                                                   gCTGCCGCATGATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGCAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCCTCGGCCAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCCGCTTTGCGGCTATAATAACAtt                                                                                                                                                >  1:662391/1‑275 (MQ=255)
                                                                                                                                      cGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTAAGCAGCCGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCAACGGCGCGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTCTAAGCGATAAATTTACCGCCAGCCGCCTATGCGGCATTAATAAAATTACGATGATGCTGTGAAGGTTAAACCACTTCGCTAGAAGAGATGAGCAGTAGTTCTNcacccccccgc                                                                             >  2:84515/1‑265 (MQ=255)
                                                                                                                                                  ctgcCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTCAACGATGCCAGAATCAGCCAGCATCTTCTCCGTCTCGATGTGCCCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGGAGTTTTTCCACTCCCTTAATTGCAGATg                                                                   >  2:633376/1‑273 (MQ=255)
                                                                                                                                                          gcgcTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCAt                                                                                                                                                                                                             >  2:567955/1‑127 (MQ=255)
                                                                                                                                                                     gTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGc                                                                                                                                                                                                        >  1:394452/1‑121 (MQ=255)
                                                                                                                                                                         ttttCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGc                                                                                                                                                                                                    <  2:394446/121‑1 (MQ=255)
                                                                                                                                                                              gtataagggacagTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCATCGCCGTAGTCAGcc                                      <  1:28545/262‑1 (MQ=255)
                                                                                                                                                                                  tACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGtttt                                                                                         >  1:75775/1‑219 (MQ=255)
                                                                                                                                                                                  tACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCa                                                     >  2:85744/1‑255 (MQ=255)
                                                                                                                                                                                  tACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCa                                                     <  1:85745/255‑1 (MQ=255)
                                                                                                                                                                                    cctgtcttggcgcAGCAGTGTGTAAACGTTGCCAGATTCAGCAGGCATTTTCTCCGTCTCGATGGGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTAAAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATGACGATGCGGCGGGGCACGTTGACCCACTTCGCTGGAAGAGAGCAGCAGTAGTTTTGACACTCCCTGAAGTGCAGATGTCAGTGCGGCTGCATCGCCGTAGTCAGCCTGACGCa                               <  1:84516/266‑1 (MQ=255)
                                                                                                                                                                                     tcgccggcgcgcAGGAGGGTGTAAACGGTGCCAGAATCAGCCAGCTGTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGGGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCATCGCCGTAGTCAGCCTGACGCa                               <  2:662382/266‑1 (MQ=255)
                                                                                                                                                                                      aGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCa                                                                                     <  2:75774/219‑1 (MQ=255)
                                                                                                                                                                                       gCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGt                                                                  >  2:69473/1‑237 (MQ=255)
                                                                                                                                                                                           ttGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGt                                                              <  1:69474/237‑1 (MQ=255)
                                                                                                                                                                                            tGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTAGGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGATTTTCAACTCACTGAAGTGCAGATGACAGAGCGGCCTCATCGACGTAGACCGCCTTAcctattgtaagt                       >  2:138699/1‑264 (MQ=255)
                                                                                                                                                                                            tGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACACACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCATCGCCGTAGTCAGCCTGa                                   >  1:404521/1‑263 (MQ=255)
                                                                                                                                                                                              cgcAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATCACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCAACTCCCTGAAGTGCAGAAGTCAGAGCGGCTTCATCGACGTAGTCAGACTGACGCACGGGAATgtc                     >  1:632460/1‑273 (MQ=255)
                                                                                                                                                                                                cagcagGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTAt                                                                                                                                                                                                                   <  2:241763/83‑1 (MQ=255)
                                                                                                                                                                                                        tgtAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACAtt                                                                                                                                                >  2:37592/1‑142 (MQ=255)
                                                                                                                                                                                                         gtAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCAGCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTTGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCAc                                                                                                                     <  1:48086/168‑1 (MQ=255)
                                                                                                                                                                                                            aaCGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGa                                                                                                                                            <  1:37592/142‑1 (MQ=255)
                                                                                                                                                                                                             aCGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATcagca                                                                                                 >  1:84103/1‑184 (MQ=255)
                                                                                                                                                                                                                aTGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGc                                                                                                                                                                            >  1:271517/1‑106 (MQ=255)
                                                                                                                                                                                                                aTGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCCTTAATAACATTACGAtgctg                                                                                                                                       >  1:14027/1‑143 (MQ=255)
                                                                                                                                                                                                                 tGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCagtag                                                                                             <  2:84102/184‑1 (MQ=255)
                                                                                                                                                                                                                 tGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTACCTGCAt                                                                                                                                                                                                             >  2:289644/1‑72 (MQ=255)
                                                                                                                                                                                                                  gCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGTTATCTGCATGTAGCAGGCGGGTATAAGAGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTGTTTCTACTCCCTGAAGTGCAGATGTTAGGACGGCCTACTCGCCGGAGTCAGCATCGCGCCCGGTAATGCCGTGGCCTGGCAgggcctgg  >  2:269153/1‑274 (MQ=255)
                                                                                                                                                                                                                  gCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAg                                                                                                                                                                                                         >  2:654207/1‑75 (MQ=255)
                                                                                                                                                                                                                  gCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGAtgc                                                                                                                                         >  1:481510/1‑139 (MQ=255)
                                                                                                                                                                                                                  gCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGCAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCCGATGTCAGTGCGGCTTCATATCCGTAGTCAGCCTGACGACCAGTAATGCCTTGTGCTGCCAgggcttgg  >  1:256078/1‑274 (MQ=255)
                                                                                                                                                                                                                  gCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTccc                                                                                >  1:210295/1‑196 (MQ=255)
                                                                                                                                                                                                                    cAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAgc                                                                                                                                                                        <  2:271513/106‑1 (MQ=255)
                                                                                                                                                                                                                    cAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCggg                                                                                                                                   <  2:14027/143‑1 (MQ=255)
                                                                                                                                                                                                                     aGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTACCTGCATGTAg                                                                                                                                                                                                         <  1:289648/72‑1 (MQ=255)
                                                                                                                                                                                                                      gAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAgg                                                                                                                                                                                                     <  1:654215/75‑1 (MQ=255)
                                                                                                                                                                                                                      gAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCg                                                                                                                                     <  2:481504/139‑1 (MQ=255)
                                                                                                                                                                                                                      gAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGaa                                                                            <  2:210292/196‑1 (MQ=255)
                                                                                                                                                                                                                         tCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGc                                                                                                                                                                                          >  1:230286/1‑83 (MQ=255)
                                                                                                                                                                                                                             ccagcATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATa                                                                                                                                                                                      <  2:230283/83‑1 (MQ=255)
                                                                                                                                                                                                                                 cATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCAc                                                                                                                                                                              >  1:647416/1‑87 (MQ=255)
                                                                                                                                                                                                                                     ttCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCa                                                                                                                                                                          <  2:647408/87‑1 (MQ=255)
                                                                                                                                                                                                                                           gTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTaa                                                                                                                                                                                                         >  1:168226/1‑49 (MQ=255)
                                                                                                                                                                                                                                               cGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAgg                                                                                                                                                                                                     <  2:168224/50‑1 (MQ=255)
                                                                                                                                                                                                                                               cGATGTGCTCAGCGGCGAGGCCGAGCGGGGANGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGGGGCTTCCTCGCCGTAGTCAGCCTGACGc                                <  1:502030/215‑1 (MQ=255)
                                                                                                                                                                                                                                                      cTCATCGGCGAGGCCGAGCGGGGAGGTACCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACt                                                                                                                    >  2:457891/1‑124 (MQ=255)
                                                                                                                                                                                                                                                          tCGGCGAGGCCGAGCGGGGAGGTACCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGc                                                                                                                <  1:457897/124‑1 (MQ=255)
                                                                                                                                                                                                                                                              cGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGgtt                                                                                          >  1:617737/1‑142 (MQ=255)
                                                                                                                                                                                                                                                                  gCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTcc                                                                                      <  2:617729/142‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aGCGGGGAGGTATCTGCATGTAGCTGGCGTGTATAAGCGATAAATTTCACGCCAGCCGCCtt                                                                                                                                                                  >  1:504730/1‑62 (MQ=255)
                                                                                                                                                                                                                                                                          gggAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCg                                                                                                                                                              <  2:504724/62‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 aTCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATcagca                                                                                                 >  1:86371/1‑116 (MQ=255)
                                                                                                                                                                                                                                                                                 aTCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGttt                                                                                          >  2:3899/1‑123 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                 
GGCACTATCGCCCGCCAGTTCGTAAACCTTGCCTTCGTGACCGGCTTCGCTAATCACGCGTGCCGCAGCTGCCGCATAATCTGCCCGCGTTGCTGAGGCGATTTTGCCATCGCCCGCCGCACCGATAAATACGCCGTGTTCCAGTGCTGCCGGGGCGCTGGCGAGGTAGTTTTCGCTGTACCAGCCGTTGCGCAGCAGGGTGTAAACGATGCCAGAATCAGCCAGCATTTTCTCCGTCTCGATGTGCTCATCGGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGCTGGTATAAGCGATAAATTTCACGCCAGCCGCCTTTGCGGCATTAATAACATTACGATGCTGCGGGGCACGTTGACCCACTTCGCTGGAAGAGATCAGCAGTAGTTTTTCCACTCCCTGAAGTGCAGATGTCAGTGCGGCTTCATCGCCGTAGTCAGCCTGACGCACGGTAATGCCTTGTGCTGCCAGGGCTTGG  >  CP009273/4423245‑4423728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑