| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,611,723 | 0 | G | A | 25.4% | 110.5 / 12.9 | 57 | K196K (AAG→AAA) | nikE | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (7/34); new base A (13/1); total (20/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAAGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAAT > CP009273/3611590‑3611853 | tAAGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCt < 1:4022363/136‑1 (MQ=255)tAAGCGGCGGCCAGCGCCCGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTac < 1:2848731/138‑1 (MQ=255) aGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTacaac < 1:1047689/139‑1 (MQ=255) cggcggCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCATTCGCCTGCTGGAAAAACTacaacaa > 1:97437/1‑139 (MQ=255) ccAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTacaacaa < 1:5032333/133‑1 (MQ=255) tCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTgg < 1:2680470/136‑1 (MQ=255) cgcgTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCATTCGCCTGCTGAAAAAACTACAAAAAAAGTTTGGCAcccg > 1:2453420/1‑135 (MQ=255) gTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCgcctgc < 1:4971520/138‑1 (MQ=255) cTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCCTTCGCCTTCTGTAAAAACTGCCACAACAGTTTTGCACCgcctgcccg > 1:2573866/1‑137 (MQ=255) gCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGtt < 1:2485418/139‑1 (MQ=255) cgcgcgCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCAccc < 1:1307395/139‑1 (MQ=255) tcgcgcTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCa < 1:4054854/138‑1 (MQ=255) gcgcgcTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCa < 1:1023599/139‑1 (MQ=255) gcccgttGGCCGGCGGACCGAAACTACTGATTGTGGATTAAGCCCGTTCTAACCTTGGTCCCTTTTTACAGGCTGGTGTCATTCGCCTCCTGAAAAAACTACAACAACAGTTTGGCATCGCCTGCCTGTTCATCACCCa < 1:796816/133‑1 (MQ=255) cgcgcTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTTGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCAc > 1:1199646/1‑139 (MQ=255) cgcgcTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCAATTGCCTGCTGAAAAAACTACAACAAAAGGTTGGCACCGCCTGCCTGTTCATTACCCAc > 1:318368/1‑139 (MQ=255) tGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGGGTTACAGGCGGGGGGCATTCGCCTGCTTAAAAAACTACAAAAAAAGTTTGGGACCGGCTGCCTGTTTATCACCCACGACtt > 1:1200616/1‑139 (MQ=255) cGGTTGAACCCCAACTTCTGATTTTGGATTAAGCCGTTTTTAACCTTGATCTCGTTTTACAGGCGGGTGTCTTTCGCCTCCTGAAAAATCTCCAACAACCGTTTGGCACCGCCTGCCTGTTCATCACCCCCGACTTAc < 1:2095254/138‑1 (MQ=255) tCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTacaacaa < 1:3844811/95‑1 (MQ=255) tCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGcc < 1:2901047/138‑1 (MQ=255) aaCTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACg < 1:962708/139‑1 (MQ=255) actactGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCAATCGCCTGCTGAAAAAAATACAAAAAAAGGTTggga > 1:1263478/1‑93 (MQ=255) tactGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑Ctt < 1:1000920/139‑1 (MQ=255) tGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGGTATTTGCCTTCTTAAAAAACCACAACAACAATTTGTGGCCGCCCTCCCTTTCCTCCCCCCCACCTTTCGCCTGGTAGAACG‑cgctt > 1:3572178/1‑134 (MQ=255) ttCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTgg < 1:3931867/85‑1 (MQ=255) ttCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCgcc < 1:4984886/92‑1 (MQ=255) tCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAg < 1:3232720/139‑1 (MQ=255) tGGATGAAGCCGTTTCTACCCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAATCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCg < 1:1642672/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:4692085/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:875372/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:990461/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:1695286/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:2467091/139‑1 (MQ=255) gAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:2639052/139‑1 (MQ=255) aaGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:3562084/138‑1 (MQ=255) aaGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTTATTCGCCTTCTTAAAAAAATACAACAAAAGTTTGGGCCCCCCTGCCCGTTCATCCCCCCCGACCTATGCCTCGTGAAAAG‑CTTTTGCCAGCGGGTaaa > 1:1306080/1‑138 (MQ=255) aGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGTGGTCATTCCCCTGCTGAAAAAGCTACAACAACAGGTTGGCCCCGCCCGCCTGTTTATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGGAATg > 1:1513488/1‑139 (MQ=255) aGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGGGTCAGTTGCCTTCTGAAAAAACTACAACAAAAGTTTTGCACCGCCCGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTTCCAGCGGGTAATg > 1:980345/1‑139 (MQ=255) gCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTTATTTGCCTTCTGAAAAAACTACCACAACAGGTTTGCACCGCCTGCCTGTTCCTCACCCACGACTTACGCCTGGTAGAACG‑CTTTTTGCCGCGGGTAATgg > 1:650485/1‑139 (MQ=255) tCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCa < 1:3757763/122‑1 (MQ=255) tAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGAc < 1:1989834/139‑1 (MQ=255) aaCCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACa < 1:1563749/139‑1 (MQ=255) aCCTTGATCTCGTGTTACAGGCGGAGGTGGTTTACCTGCGGAAAAAAATAAAACAAAACTGTGGGGCCGCCCTCCCGTTCATCACCAACACCTTACGCCAGGTGGAACGCCTTTTGCC‑GCGGGGAAGGGATATgttcaa > 1:4590064/1‑134 (MQ=255) aTCTCGTGTTACAGGCGGGTGGCAATTGCCTGCTGAAAAAACTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTAAGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATg > 1:1176283/1‑122 (MQ=255) gtgtTACAGGCGGGTGTCATTCGCCTGCTGAAAAAACTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGGAGAACG‑CTTTTTCCCGCGGGGAATGGTTTTGGACAACCGACAAAtcg > 1:607393/1‑139 (MQ=255) tgtTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTaa < 1:284776/114‑1 (MQ=255) tgtTACAGGCGGGTGTCATTCGCCCTCTGAAAAAGCTACCACAACAGTTTGGCACCGCCCTCCTGTTCCTCACCCACGACCTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGGTATGGACAACGGACAAAtcg > 1:2364183/1‑138 (MQ=255) gtTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCa < 1:34165/105‑1 (MQ=255) gtTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAAtcgtc < 1:1231328/139‑1 (MQ=255) gtgtCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACtt < 1:3609482/70‑1 (MQ=255) gtgtCATTCGCCTCCTGAAAAAGCTACAACACCAGTTTGGCGCCGCCTGCCTGTTCATCGCCCACGACGTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAgg < 1:469777/138‑1 (MQ=255) cATCCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCCCCCACGACTTAc < 1:2278234/68‑1 (MQ=255) ttCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTggg > 1:2557405/1‑139 (MQ=255) ccTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTa > 1:1027773/1‑70 (MQ=255) ccTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGGGGTGGgagag > 1:2356297/1‑139 (MQ=255) ctgAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGa > 1:4857485/1‑68 (MQ=255) tgAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAAt < 1:3781086/138‑1 (MQ=255) | TAAGCGGCGGCCAGCTCCAGCGCGTCTGCCTGGCTCGCGCGCTGGCGGTCGAACCGAAACTACTGATTCTGGATGAAGCCGTTTCTAACCTTGATCTCGTGTTACAGGCGGGTGTCATTCGCCTGCTGAAAAAGCTACAACAACAGTTTGGCACCGCCTGCCTGTTCATCACCCACGACTTACGCCTGGTAGAACG‑CTTTTGCCAGCGGGTAATGGTTATGGACAACGGACAAATCGTCGAAACCCAGGTGGTGGGAGAGAAAT > CP009273/3611590‑3611853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |