Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	155,132	C→G	R161P (CGC→CCC)	pcnB ←	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	155,132	0	C	G	98.7%	285.5 / NA	77	R161P (CGC→CCC)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (43/33);  minor base T (1/0);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGGTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGAT  >  CP009273/155004‑155262                                                                                                                                 |                                                                                                                                   cACGGTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGTGGCGCTGGGc                                                                                                                          >  1:3269660/1‑139 (MQ=255) cACGGTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGc                                                                                                                          >  1:1316951/1‑139 (MQ=255)  aCGGTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCg                                                                                                                         <  1:1763022/139‑1 (MQ=255)    ggTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtc                                                                                                                       <  1:960178/139‑1 (MQ=255)    ggTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtc                                                                                                                       <  1:3898202/139‑1 (MQ=255)     gTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                                                                                                      >  1:3152554/1‑139 (MQ=255)      tAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                                                                                                      >  1:1000513/1‑138 (MQ=255)         cgcgTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                                                                                                   >  1:1491898/1‑138 (MQ=255)         cgcgTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                                                                                                   >  1:3231046/1‑138 (MQ=255)         cgcgTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                                                                                                   >  1:3754903/1‑138 (MQ=255)                      tACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGa                                                                                                      <  1:2562229/138‑1 (MQ=255)                       aCCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGaaa                                                                                                    >  1:2398468/1‑139 (MQ=255)                                aCGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTc                                                                                            >  1:2832374/1‑138 (MQ=255)                                aCGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTc                                                                                            >  1:378766/1‑138 (MQ=255)                                aCGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTc                                                                                            >  1:267778/1‑138 (MQ=255)                                  ggATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcgcg                                                                                         <  1:648807/139‑1 (MQ=255)                                        cGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcagca                                                                                    >  1:4895196/1‑138 (MQ=255)                                          ccGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACa                                                                                 >  1:5006677/1‑139 (MQ=255)                                           cGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACAt                                                                                <  1:4370983/139‑1 (MQ=255)                                             tCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCg                                                                                                                         >  1:936957/1‑96 (MQ=255)                                                      tCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGcc                                                                     <  1:4659696/139‑1 (MQ=255)                                                          tCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGAGGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCcgcg                                                                 >  1:4653400/1‑139 (MQ=255)                                                               ccgccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTggg                                                            >  1:1728457/1‑139 (MQ=255)                                                                 gccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGa                                                                                                               <  1:1165531/86‑1 (MQ=255)                                                                 gccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGGGGTGGGAg                                                          >  1:3644571/1‑139 (MQ=255)                                                                 gccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAg                                                          >  1:2139914/1‑139 (MQ=255)                                                                  ccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTGgggggg                                                         >  1:4832877/1‑139 (MQ=255)                                                                          aTCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGACGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCgg                                                 >  1:986907/1‑139 (MQ=255)                                                                           tCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGaa                                                                                                     >  1:3747026/1‑86 (MQ=255)                                                                               ggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCt                                            <  1:2380915/139‑1 (MQ=255)                                                                                gacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTg                                           >  1:2190862/1‑139 (MQ=255)                                                                                   ggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACg                                        >  1:2400514/1‑139 (MQ=255)                                                                                     tAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGt                                       <  1:462879/138‑1 (MQ=255)                                                                                         aTCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCt                                  <  1:4770307/139‑1 (MQ=255)                                                                                             gCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgt                              <  1:3000356/139‑1 (MQ=255)                                                                                              cTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             >  1:2295276/1‑139 (MQ=255)                                                                                               tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             <  1:3582285/138‑1 (MQ=255)                                                                                               tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             <  1:1006355/138‑1 (MQ=255)                                                                                               tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             <  1:2184187/138‑1 (MQ=255)                                                                                               tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             <  1:3145351/138‑1 (MQ=255)                                                                                               tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                             <  1:2850547/138‑1 (MQ=255)                                                                                                aCGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtggt                           <  1:2427708/139‑1 (MQ=255)                                                                                                    tGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATgg                                                                                                            <  1:602201/54‑1 (MQ=255)                                                                                                    tGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTcc                       >  1:1394032/1‑139 (MQ=255)                                                                                                      tAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGcc                                                                     <  1:4336257/91‑1 (MQ=255)                                                                                                        aTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGcc                                                                                                        <  1:748971/54‑1 (MQ=255)                                                                                                        aTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACgg                   <  1:4072798/139‑1 (MQ=255)                                                                                                           cAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAg                >  1:3930540/1‑139 (MQ=255)                                                                                                             ggCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAgg               >  1:2785658/1‑138 (MQ=255)                                                                                                              gcggTTGATAGTGAAATCGGGGAGGTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:3909985/4‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAt                                                                                                  >  1:3184494/1‑54 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTg                                                    <  1:153294/100‑1 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:794560/1‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:755411/1‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:4668771/1‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:2182103/1‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:4818773/1‑139 (MQ=255)                                                                                                              gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc             >  1:1396425/1‑139 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGa                                                           >  1:1755925/1‑91 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           >  1:943567/1‑139 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           >  1:111349/1‑139 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           >  1:702224/1‑139 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           <  1:1062936/139‑1 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           <  1:4719465/139‑1 (MQ=255)                                                                                                                tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc           >  1:4881202/1‑139 (MQ=255)                                                                                                                  ttGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAtgttg                                                                                              >  1:3331038/1‑54 (MQ=255)                                                                                                                  ttGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGc                                             <  1:752513/103‑1 (MQ=255)                                                                                                                   tGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa         <  1:583912/138‑1 (MQ=255)                                                                                                                   tGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa         <  1:2302024/138‑1 (MQ=255)                                                                                                                   tGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa         <  1:2961351/138‑1 (MQ=255)                                                                                                                   tGATAGTGAAATCCGGGCGCTTGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa         <  1:2073733/138‑1 (MQ=255)                                                                                                                   tGATAGGGAAATCGGGGCGCTGGGCGTCTTCATCGATGGAGCCGAAAAAGTTGTCGCGCAGGAACATGCCGAGTTGCCCGCGGTGGGAGGTCGGGCGGTCGCTGACGTTACCTACGAGGTGTCCACGGAAGGTCGCaa         <  1:3373976/138‑1 (MQ=255)                                                                                                                        gTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGa                                          >  1:2736220/1‑100 (MQ=255)                                                                                                                         tGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGc                                                                      <  1:3367863/71‑1 (MQ=255)                                                                                                                         tGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGAt  >  1:2196605/1‑139 (MQ=255)                                                                                                                           aaaTCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGt                                       <  1:639860/100‑1 (MQ=255)                                                                                                                            aaTCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTAcc                                   >  1:3334578/1‑103 (MQ=255)                                                                                                                                 |                                                                                                                                   CACGGTAGCGCGTTTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGAT  >  CP009273/155004‑155262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑