Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,173,770	T→G	D866E (GAT→GAG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,173,770	0	T	G	100.0%	305.2 / NA	81	D866E (GAT→GAG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/39);  total (42/39)

TGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAA  >  CP009273/4173635‑4173899                                                                                                                                        |                                                                                                                                  tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGaa                                                                                                                                >  1:3851838/1‑139 (MQ=255) tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAAAGAGGGTGAAGCTGCGCTCTCCAAACTGGAGGaa                                                                                                                                >  1:3353419/1‑139 (MQ=255) tgttgtTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTACCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCGGCGCTCGCCAAACTGGAGGaa                                                                                                                                >  1:5099710/1‑139 (MQ=255)   ttgttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt                                                                                                                               >  1:3290164/1‑138 (MQ=255)    tgttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATc                                                                                                                              <  1:4663343/138‑1 (MQ=255)     gttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCg                                                                                                                            <  1:197720/139‑1 (MQ=255)     gttCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCg                                                                                                                            <  1:3656823/139‑1 (MQ=255)       tCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGt                                                                                                                          <  1:2616804/139‑1 (MQ=255)       tCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTACGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGt                                                                                                                          <  1:1215239/139‑1 (MQ=255)        cAGGAAGACCGTTTCACCTCCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGATCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTa                                                                                                                         <  1:3348887/139‑1 (MQ=255)            aaGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATc                                                                                                                       <  1:2060152/137‑1 (MQ=255)            aaGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATc                                                                                                                       <  1:2783618/137‑1 (MQ=255)                ccGTTTCACCACCATCCACATTCAGGTACTGGCGTGTGGGTCCCGTGCCCCCAAGCTGGGTCCGGAAGAGATCACCGCTGACCTCCCGCACGTGGGTGAAGCTGCGCTTCCCAAACTGGCGGAATCCGGTATCGTTTAc                                                                                                                 >  1:3427832/1‑139 (MQ=255)                ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTAc                                                                                                                 >  1:1519074/1‑139 (MQ=255)                ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTAc                                                                                                                 >  1:4639836/1‑139 (MQ=255)                ccGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTAc                                                                                                                 >  1:2168242/1‑139 (MQ=255)                  gTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACAt                                                                                                               <  1:4190859/139‑1 (MQ=255)                    ttCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATc                                                                                                                       <  1:2692703/129‑1 (MQ=255)                            caTCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTa                                                                                                                         <  1:2461390/119‑1 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTg                                                                                                             >  1:109696/1‑129 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAgg                                                                                                   >  1:2890364/1‑138 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:1425028/1‑139 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:3563046/1‑139 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:1633758/1‑139 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:2356130/1‑139 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:2010963/1‑139 (MQ=255)                              tCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGt                                                                                                   >  1:2502407/1‑139 (MQ=255)                                cacaTTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGa                                                                                                 <  1:1004934/139‑1 (MQ=255)                                 acaTTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGAc                                                                                                <  1:2634531/139‑1 (MQ=255)                                      cAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACAt                                                                                                               >  1:5040292/1‑119 (MQ=255)                                        ggAACTGGCGTGTGTGTCCCGTGACACCAAGCCGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGCGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGCGACCGGTGGc                                                                                         >  1:4624174/1‑139 (MQ=255)                                         gAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCg                                                                                        >  1:1153503/1‑139 (MQ=255)                                          aaCTGGCGTGTGTGTCCCGTGACACCCAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTgg                                                                                          <  1:4646839/136‑1 (MQ=255)                                          aaCTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTgg                                                                                          <  1:5097378/136‑1 (MQ=255)                                          aaCTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTgg                                                                                          <  1:5013507/136‑1 (MQ=255)                                            cTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTACAAACTTGTGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACa                                                                                     >  1:4040093/1‑139 (MQ=255)                                               gCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAt                                                                                    >  1:739670/1‑137 (MQ=255)                                                cGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAt                                                                                    >  1:2186285/1‑136 (MQ=255)                                                 gtgtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCt                                                                                 <  1:1968476/138‑1 (MQ=255)                                                   gtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTa                                                                                                                         >  1:3487609/1‑96 (MQ=255)                                                   gtgtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACAt                                                                                                               <  1:1388192/106‑1 (MQ=255)                                                   gggtgtCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggt                                                                              >  1:4548387/3‑139 (MQ=255)                                                             tGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGaa                                                                                                     >  1:3969278/1‑106 (MQ=255)                                                                  ccAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTa                                                                                                                         <  1:1831992/81‑1 (MQ=255)                                                                  ccAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGcc                                                               >  1:3517568/1‑139 (MQ=255)                                                                   cAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGCACGTGGGAGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGGGGCG‑CATTCTGGGTGGTAAGGTAACGCCGa                                                             >  1:5080234/1‑139 (MQ=255)                                                                     aGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAtt                                                                                   <  1:4210665/116‑1 (MQ=255)                                                                     aGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGaa                                                            <  1:2507057/139‑1 (MQ=255)                                                                     aGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGaa                                                            <  1:4935204/139‑1 (MQ=255)                                                                     aGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGaa                                                            >  1:2556028/1‑139 (MQ=255)                                                                          ggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTg                                                       >  1:1721017/1‑139 (MQ=255)                                                                          ggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTg                                                       >  1:268181/1‑139 (MQ=255)                                                                            tCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACAt                                                                                                               >  1:4412211/1‑81 (MQ=255)                                                                               ggAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTggta                                                                         >  1:1630043/1‑116 (MQ=255)                                                                                gAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTc                                                 >  1:189862/1‑139 (MQ=255)                                                                                     gaTCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCt                                             <  1:935756/138‑1 (MQ=255)                                                                                     gaTCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCt                                             <  1:2510967/138‑1 (MQ=255)                                                                                      aTCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTa                                                                                                                         >  1:3532044/1‑61 (MQ=255)                                                                                         aCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACGGGTGGCGACATTCTGGTTGGAAAGGAAACGCCGAAAGGTGAAACTCAGCTGAcc                                         >  1:2042840/1‑138 (MQ=255)                                                                                          ccGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAcccc                                       <  1:2772728/139‑1 (MQ=255)                                                                                          ccGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAcccc                                       <  1:4747730/139‑1 (MQ=255)                                                                                              tGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCaga                                    <  1:2125586/138‑1 (MQ=255)                                                                                                aCATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGAcccc                                       <  1:489668/133‑1 (MQ=255)                                                                                                    cccGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtg                                                                                <  1:720939/88‑1 (MQ=255)                                                                                                      cGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAAct                           <  1:2481614/139‑1 (MQ=255)                                                                                                       gAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAAct                           >  1:4685765/1‑138 (MQ=255)                                                                                                        aaCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAAct                           >  1:2035375/1‑137 (MQ=255)                                                                                                        aaCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAAct                           >  1:4299294/1‑137 (MQ=255)                                                                                                         aCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGTTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAActgct                        <  1:1459136/139‑1 (MQ=255)                                                                                                         aCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAActgct                        <  1:4671183/139‑1 (MQ=255)                                                                                                         aCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAActgc                         <  1:4301411/138‑1 (MQ=255)                                                                                                         aCGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAActgc                         <  1:4615112/138‑1 (MQ=255)                                                                                                          cGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGAAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGaaaaa                             >  1:3072089/1‑133 (MQ=255)                                                                                                             gggTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCtgcgt                    <  1:4006049/139‑1 (MQ=255)                                                                                                              ggTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTggtaagg                                                                      >  1:3303052/1‑88 (MQ=255)                                                                                                               gTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCtgcgtgc                  >  1:1061656/1‑139 (MQ=255)                                                                                                                        cgcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGGGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCgg         >  1:1598112/1‑139 (MQ=255)                                                                                                                         gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGt        <  1:981308/139‑1 (MQ=255)                                                                                                                               cAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCgg                                                                                             <  1:959583/48‑1 (MQ=255)                                                                                                                               cAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGTTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGaaa  <  1:1680287/139‑1 (MQ=255)                                                                                                                                  aCTGGAGGACTCCGGTCTCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCt                                             <  1:4237869/93‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCGAAAGGTGAAACTCAGCTGACCCCAGAAGAAAAACTGCTGCGTGCGATCTTCGGTGAGAAA  >  CP009273/4173635‑4173899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑