BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	188,740	C→G	I133M (ATC→ATG)	pyrH →	uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	188,740	0	C	G	97.9%	354.0 / ‑0.9	97	I133M (ATC→ATG)	pyrH	uridylate kinase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (52/42); total (54/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

GGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCG  >  CP009273/188608‑188869

ggCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTcc                                                                                                                             <  1:725425/139‑1 (MQ=255)

  cAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTccg                                                                                                                            <  1:1618934/138‑1 (MQ=255)

   aaTGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTccgc                                                                                                                           <  1:682151/138‑1 (MQ=255)

       cGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGt                                                                                                                       >  1:2541082/1‑138 (MQ=255)

       cGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGt                                                                                                                       >  1:2670607/1‑138 (MQ=255)

       cGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGt                                                                                                                       >  1:600236/1‑138 (MQ=255)

           aTGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAgg                                                                                                                  >  1:4514099/1‑139 (MQ=255)

           aTGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAgg                                                                                                                  >  1:1246684/1‑139 (MQ=255)

                 tgcacCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAccc                                                                                                            >  1:992175/1‑139 (MQ=255)

                  gcacCGCGCCTATGTGACCGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCg                                                                                                           <  1:1934060/139‑1 (MQ=255)

                    acCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGt                                                                                                          <  1:1010784/138‑1 (MQ=255)

                      cgcgCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCt                                                                                                       >  1:1490912/1‑139 (MQ=255)

                      cgcgCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCt                                                                                                       >  1:498173/1‑139 (MQ=255)

                             aTGTGAACGCTCTTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTacca                                                                                                 >  1:2011164/1‑138 (MQ=255)

                             aTGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTaccac                                                                                                <  1:3740920/139‑1 (MQ=255)

                             aTGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGACGCTCTCCGCCGGTACAGGTAACCCGTTCTTTaccac                                                                                                <  1:3359882/139‑1 (MQ=255)

                                  aaCGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGAc                                                                                            >  1:1159421/1‑138 (MQ=255)

                                     gCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTca                                                                                         >  1:3171058/1‑138 (MQ=255)

                                       tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcagca                                                                                      <  1:4895279/139‑1 (MQ=255)

                                       tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcagca                                                                                      >  1:2316132/1‑139 (MQ=255)

                                       tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcagca                                                                                      >  1:18673/1‑139 (MQ=255)

                                       tCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcagca                                                                                      >  1:3190210/1‑139 (MQ=255)

                                          tCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCt                                                                                   >  1:2126914/1‑139 (MQ=255)

                                           cTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTccgc                                                                                                                           <  1:800495/98‑1 (MQ=255)

                                           cTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTc                                                                                          <  1:2904255/131‑1 (MQ=255)

                                                  ccGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCg                                                                           <  1:1150171/139‑1 (MQ=255)

                                                  ccGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCg                                                                           <  1:3080581/139‑1 (MQ=255)

                                                  ccGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCg                                                                           <  1:1226975/139‑1 (MQ=255)

                                                     cTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACCGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGc                                                                                >  1:321583/1‑131 (MQ=255)

                                                     cTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGt                                                                                                                 >  1:3382499/1‑98 (MQ=255)

                                                      tATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAc                                                                                                              <  1:3419496/100‑1 (MQ=255)

                                                        ttCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTgggt                                                                      >  1:4581626/1‑136 (MQ=255)

                                                        ttCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTa                                                                      >  1:3731676/1‑138 (MQ=255)

                                                        ttCCAGTGAAGGGGGGGGGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGGTGCGCAACAACCGTGTGGGGATGCTCTCCGCCGGTACAGGGAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTAt                                                                     <  1:1037018/139‑1 (MQ=255)

                                                           cATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCgg                                                                                                                        <  1:159576/85‑1 (MQ=255)

                                                             ttGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCg                                                                                                           <  1:3723353/96‑1 (MQ=255)

                                                             ttGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCTTGGTATCGaaa                                                                >  1:3618703/1‑139 (MQ=255)

                                                             ttGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGaaa                                                                >  1:1934002/1‑139 (MQ=255)

                                                                aaTGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTa                                                                                                    >  1:837541/1‑100 (MQ=255)

                                                                aaTGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTcag                                                                                        <  1:3390403/112‑1 (MQ=255)

                                                                  tGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGaa                                                           >  1:30098/1‑139 (MQ=255)

                                                                  tGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGaa                                                           >  1:1811901/1‑139 (MQ=255)

                                                                   ggCGTAAGCGACAGCTAAAGCTGGGCAGAAGCTATCAGCCAGTAGCGCAACACCCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACAGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAg                                                          <  1:5119864/139‑1 (MQ=255)

                                                                    gCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAcc                                                                                                             <  1:4931275/87‑1 (MQ=255)

                                                                     cgggtgCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCCACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAc                                                                                                              >  1:2742490/4‑85 (MQ=255)

                                                                     cGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGct                                                                                                                                 >  1:1061195/1‑66 (MQ=255)

                                                                     cGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTccgc                                                                                                                           <  1:1221586/72‑1 (MQ=255)

                                                                     cGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGcc                                                        >  1:1508106/1‑139 (MQ=255)

                                                                     cGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGcc                                                        >  1:3364299/1‑139 (MQ=255)

                                                                      gtgtGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCg                                                       <  1:3828042/139‑1 (MQ=255)

                                                                       tgtgCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTacca                                                                                                 >  1:1141839/1‑96 (MQ=255)

                                                                       tgtgCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGa                                                      >  1:3191258/1‑139 (MQ=255)

                                                                        gtgCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAt                                                     >  1:3541944/1‑139 (MQ=255)

                                                                        gtgCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAt                                                     >  1:1752027/1‑139 (MQ=255)

                                                                          gcgcCAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtg                                                    <  1:3825889/138‑1 (MQ=255)

                                                                          gCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCt                                                                              >  1:808416/1‑112 (MQ=255)

                                                                          gCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtg                                                    <  1:521173/138‑1 (MQ=255)

                                                                              cagctacagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTac                                                                                                   >  1:2352194/1‑87 (MQ=255)

                                                                               agctacagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGt                                                                                                                 >  1:3802994/1‑72 (MQ=255)

                                                                                gctacagctGGGCAGAAGCTATCAGCCTGTTGCTCAACAACCGTGTGGTGATGCTCTccg                                                                                                                            <  1:2422288/60‑1 (MQ=255)

                                                                                   acagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGaa                                          >  1:4338210/1‑139 (MQ=255)

                                                                                    cagctGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGaaa                                         >  1:2754774/1‑139 (MQ=255)

                                                                                        tGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCCCCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGaaa                                         <  1:499232/135‑1 (MQ=255)

                                                                                        tGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGc                                       <  1:1757880/137‑1 (MQ=255)

                                                                                        tGGGCAGAAGCTATCAGCCTGTTGCGAAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGaaa                                         <  1:3212591/135‑1 (MQ=255)

                                                                                          ggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                   >  1:3081639/1‑139 (MQ=255)

                                                                                          ggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                   >  1:717244/1‑139 (MQ=255)

                                                                                          ggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                   >  1:3655911/1‑139 (MQ=255)

                                                                                          ggCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                   >  1:1784643/1‑139 (MQ=255)

                                                                                          ggCAGAAGCTATAAGCCTGTTGCGCAACAACCTTGTGGAGATGCTCTCCGCCGGTACAgg                                                                                                                  >  1:5001263/1‑60 (MQ=255)

                                                                                                   tATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAc                                                                                                              <  1:2756605/55‑1 (MQ=255)

                                                                                                   tATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTg                            <  1:171873/137‑1 (MQ=255)

                                                                                                   tATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                          <  1:4332366/139‑1 (MQ=255)

                                                                                                   tATCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                          <  1:1074293/139‑1 (MQ=255)

                                                                                                   tATCAGCCTGTGGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTg                            <  1:3089797/137‑1 (MQ=255)

                                                                                                    aTCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACg                         >  1:1681257/1‑139 (MQ=255)

                                                                                                    aTCAGCCTGTTGCGCAACAACCGTGTGGTGATACTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGAATCAGCAGCTTGCCTGCGAGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAGAGTTGACg                         >  1:4888558/1‑139 (MQ=255)

                                                                                                     tCAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGAtgtg                                                  <  1:134261/113‑1 (MQ=255)

                                                                                                      cAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAAcc                                                                                                             <  1:456707/53‑1 (MQ=255)

                                                                                                      cAGCCTGTTGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGc                       <  1:960182/139‑1 (MQ=255)

                                                                                                      cAGCCTGTTGCGCAACAACCGTGTGGTGATGCACTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAATTTGACGGc                       <  1:3299362/139‑1 (MQ=255)

                                                                                                           tGTTGCGCAACAACCGTGTGTTGATGCTCTCCGCCGGTACAGGTTACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGtt                  <  1:269243/139‑1 (MQ=255)

                                                                                                             ttGCGCAACAACCGTGTGGTGATGGTCTCCGCCGGTACAGGTAACCCGTACTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGACGATGAGGTGCTGACAGCAACCAAAGTTGACGGCGTGtta                 >  1:3175489/1‑137 (MQ=255)

                                                                                                             ttGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTa                                                                                                    >  1:173708/1‑55 (MQ=255)

                                                                                                             ttGCGCAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAAcc                                   <  1:1190624/120‑1 (MQ=255)

                                                                                                               gcgcCACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGaa                                                                 >  1:2667723/1‑88 (MQ=255)

                                                                                                               gcgcAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAg                                        >  1:2717215/1‑113 (MQ=255)

                                                                                                                cgcAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTac                                                                                                   >  1:3039166/1‑53 (MQ=255)

                                                                                                                 gcAACAACCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCg                                                                           <  1:1952317/76‑1 (MQ=255)

                                                                                                                  caacaaCCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTAAGCAGCTTGCCTGCGGGGTATCGAAATTGAAGCCGTTGGGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTAcc              >  1:2358373/1‑136 (MQ=255)

                                                                                                                   aacaacCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCt           <  1:1152824/138‑1 (MQ=255)

                                                                                                                     caacCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGa         >  1:1049220/1‑138 (MQ=255)

                                                                                                                      aacCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATc       <  1:1655760/139‑1 (MQ=255)

                                                                                                                       acCGTGTGGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACg                         >  1:3772084/1‑120 (MQ=255)

                                                                                                                           tgtgGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGaa                                                                 >  1:4532294/1‑76 (MQ=255)

                                                                                                                           tgtgGTGATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCg  >  1:3734329/1‑139 (MQ=255)

                                                                                                                            gtggtgATGCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCg  <  1:630593/138‑1 (MQ=255)

GGCAATGCGTGATGCACTGCACCGCGCCTATGTGAACGCTCGTCTGATGTCCGCTATTCCATTGAATGGCGTGTGCGACAGCTACAGCTGGGCAGAAGCTATCAGCCTGTTGCGCAACAACCGTGTGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCG  >  CP009273/188608‑188869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`