Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 407,471 C→T W156* (TGG→TAG)  araJ ← arabinose‑inducible putative transporter, MFS family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273407,4710CT98.8% 275.5 / NA 83W156* (TGG→TAG) araJarabinose‑inducible putative transporter, MFS family
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (48/34);  total (48/35)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCG  >  CP009273/407337‑407605
                                                                                                                                      |                                                                                                                                      
gggCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCt                                                                                                                                    >  1:291610/1‑139 (MQ=255)
      ggCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCC‑AAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAAtt                                                                                                                               >  1:4956238/1‑137 (MQ=255)
         tGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCt                                                                                                                            <  1:3863943/138‑1 (MQ=255)
         tGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCt                                                                                                                            <  1:2026210/138‑1 (MQ=255)
         tGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           <  1:100118/139‑1 (MQ=255)
         tGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           <  1:192807/139‑1 (MQ=255)
         tGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           <  1:4178996/139‑1 (MQ=255)
         tGCGCAAAAAGTGAAATAGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCt                                                                                                                            <  1:3581249/138‑1 (MQ=255)
           cgcAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAACATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           >  1:2049905/1‑137 (MQ=255)
           cgcAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           >  1:1282633/1‑137 (MQ=255)
           cgcAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTg                                                                                                                           >  1:1344662/1‑137 (MQ=255)
              aaaaaGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTa                                                                                                                      <  1:3699302/139‑1 (MQ=255)
                 aaGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGTCCGATGCCAGCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGTCTTAAAt                                                                                                                   <  1:4629610/139‑1 (MQ=255)
                        aTTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTcc                                                                                                            <  1:3925861/139‑1 (MQ=255)
                             tCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCg                                                                                                       <  1:85794/139‑1 (MQ=255)
                              cgcgCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCgg                                                                                                      >  1:4375295/1‑139 (MQ=255)
                                   aGATTTCCTTTCGCCTCGTCGAGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATg                                                                                                  <  1:4519068/138‑1 (MQ=255)
                                   aGATTTCCTTTCGCCTCGTCACGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATg                                                                                                  <  1:435676/138‑1 (MQ=255)
                                     aTTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCt                                                                                                                            <  1:4081871/110‑1 (MQ=255)
                                     aTTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGa                                                                                                                          <  1:2226345/112‑1 (MQ=255)
                                      tttCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCa                                                                                              <  1:2183686/139‑1 (MQ=255)
                                               gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATa                                                                                                                  >  1:1500985/1‑110 (MQ=255)
                                               gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACg                                                                                                                >  1:4805716/1‑112 (MQ=255)
                                               gCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTg                                                                                      >  1:2955734/1‑138 (MQ=255)
                                               gCCTCGTCGCGAATATCTGGCA‑CCAAAAATAGACCGATGACATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGc                                                                                    >  1:2275104/1‑139 (MQ=255)
                                                       gcgAATATCTGGCACCC‑AAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCa                                                                            <  1:2377192/139‑1 (MQ=255)
                                                                   cACCCAAAAATAGACCGATGCCATCCCCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAAcc                                                                 >  1:841557/1‑139 (MQ=255)
                                                                      ccAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCa                                                                                              <  1:2491391/107‑1 (MQ=255)
                                                                       cAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATc                                                              >  1:4356029/1‑138 (MQ=255)
                                                                        aaaaaTAGACCGACGCCATCGCCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATGCGTACCCa                                                                                                          <  1:3470116/93‑1 (MQ=255)
                                                                         aaaaTAGACCGATGCCATCACCGCAATATTAAAAACAGAGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCAGGAAACCATcccc                                                           >  1:303681/1‑139 (MQ=255)
                                                                          aaaTAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTc                                                                             <  1:1232366/120‑1 (MQ=255)
                                                                          aaaTAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGaaa                                                                   <  1:706191/130‑1 (MQ=255)
                                                                                aCCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGc                                                                                    >  1:5070254/1‑107 (MQ=255)
                                                                                  cGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGcc                                                                                                >  1:888229/1‑93 (MQ=255)
                                                                                    aTGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCt                                                                                                                            <  1:4950837/63‑1 (MQ=255)
                                                                                    aTGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGaaa                                                                   >  1:3813751/1‑120 (MQ=255)
                                                                                    aTGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGc                                                         >  1:3288326/1‑130 (MQ=255)
                                                                                     tGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCAcggcggcg                                               >  1:2169163/1‑139 (MQ=255)
                                                                                     tGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCAcggcggcg                                               >  1:3719775/1‑139 (MQ=255)
                                                                                        cATCCCCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTg                                            <  1:1795017/139‑1 (MQ=255)
                                                                                              cGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATa                                                                                                                  >  1:2371783/1‑63 (MQ=255)
                                                                                                cAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTcc                                    >  1:1324834/1‑139 (MQ=255)
                                                                                                cAATATAAAAAACAGCGACAAATAAAAAGGTGTAACGCTAGCTAAATCCCTGACTAAAATACGTCCCCAGCGGAAGCCCCAGCAAATTGGCGACTGTCACCCCGAAACCCATCCCCGCCACGGCGGCGGTGACTTTTcc                                    <  1:3088881/139‑1 (MQ=255)
                                                                                                  atatTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCgg                                  >  1:2370023/1‑139 (MQ=255)
                                                                                                   tatTAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCAAAATTCCTGACTTAAATACGTCCCCAGCGGAATGCCCAGCAAATGGGCGTCTGTCATCCCGGAACCCATCCCCGCCACGGCGGCGGt                                             <  1:254999/127‑1 (MQ=255)
                                                                                                     ttAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACt                                                                                                                        <  1:397565/50‑1 (MQ=255)
                                                                                                     ttAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGa                                                                                                     <  1:2831411/69‑1 (MQ=255)
                                                                                                      tAAAAACAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGttt                              >  1:3948300/1‑139 (MQ=255)
                                                                                                        aaaaCAGCGATCAATAAAAAGGTGTAACGCTAGCTAAATCCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGa                            <  1:2043556/139‑1 (MQ=255)
                                                                                                             agtgATCAATAAAAAGGTATAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGCCTTTTCCGGGTTTGATAAtt                       >  1:2229548/4‑139 (MQ=255)
                                                                                                             aGCGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCg                                   >  1:2837784/1‑127 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGtt                                                                                                              >  1:2980117/1‑50 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCa                                                                                           >  1:248619/1‑69 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAtttt                     >  1:2130577/1‑139 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAtttt                     >  1:3111744/1‑139 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAtttt                     >  1:1645425/1‑139 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGGTTGATAAtttt                     >  1:3387157/1‑139 (MQ=255)
                                                                                                               cGATCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGGTTGATAAtttt                     >  1:3524783/1‑139 (MQ=255)
                                                                                                               cGATCAATAAAAAGGGGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCCAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGGTTGATAAtttt                     >  1:4571733/1‑139 (MQ=255)
                                                                                                                gATCAATAAAAAGGTGTAACGCTAGCTAAATCCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATGGGCGCCTGTCATCCCGGAAACCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAttttt                    <  1:805163/139‑1 (MQ=255)
                                                                                                                gATAAAAAAAAGGGTGTACCCCTAGCAAAATCCCTACCTTAAATCCGTCCCCAGCGAAAGCCCCAGCAAATTGGCGACTGCCACCCCGAAAACCACCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAAtttt                     <  1:1367157/138‑1 (MQ=255)
                                                                                                                  tCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATATGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGa                  >  1:1346432/1‑139 (MQ=255)
                                                                                                                  tCAATAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGa                  >  1:4513436/1‑139 (MQ=255)
                                                                                                                    aaTAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGCCTGTCACCCCGGAAACCACCCCCGCCAcg                                                     <  1:4960447/102‑1 (MQ=255)
                                                                                                                    aaTAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGaaa                                                                   <  1:4449783/88‑1 (MQ=255)
                                                                                                                      tAAAAAGGTGTAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGa                                                                                                     <  1:5030288/52‑1 (MQ=255)
                                                                                                                        aaaaGGTGTAACGCTAGCTAAATCCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAacac            <  1:529368/139‑1 (MQ=255)
                                                                                                                           aGGGGTATCCCCAGATAAATTCCTGCCTTAAATTGGTCCCCGGCGGACTCCCCAGCAACTTGGCGACTGTCACCCCGAAACCCCCCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATCTTTGATAACACGAt         <  1:3927626/139‑1 (MQ=255)
                                                                                                                             gtgtAACGCTAGCTAAATCCCTGACTTAAACACGTCCCCGGCGGAATCCCCAGCAAATGGGCGACTTTCACCCCGGAACCCACCCCCGCCACGGCGGCGGTGACTTTTcc                                    <  1:3627328/110‑1 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGc                                                         >  1:1869629/1‑88 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGa                                           >  1:2381409/1‑102 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATc        >  1:233123/1‑137 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATc        >  1:487155/1‑137 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATc        >  1:2138737/1‑137 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATc        >  1:317306/1‑137 (MQ=255)
                                                                                                                              tgtAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCg       >  1:4465919/1‑138 (MQ=255)
                                                                                                                                tAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCa                                                                                           >  1:2451369/1‑52 (MQ=255)
                                                                                                                                tAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt     >  1:2491895/1‑138 (MQ=255)
                                                                                                                                tAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt     >  1:2929652/1‑138 (MQ=255)
                                                                                                                                tAACGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCAAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt     >  1:525318/1‑138 (MQ=255)
                                                                                                                                tAACGCTAGCTAAATTCCTGAATTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCAGCCACGGCGGAGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCt     >  1:2060684/1‑138 (MQ=255)
                                                                                                                                  aCGCTAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCg  >  1:3808903/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
GGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATAAAAAGGTGTAACGCCAGCTAAATTCCTGACTTAAATACGTTCCCAGCGGAATGCCCAGCAAATTGGCGACTGTCATCCCGGAAACCATCCCCGCCACGGCGGCGGTGACTTTTCCGGGTTTGATAATTTTTGATAACACGATCGCTCCG  >  CP009273/407337‑407605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑