Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,208,093	T→C	F563S (TTC→TCC)	rpoD →	RNA polymerase, sigma 70 (sigma D) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,208,093	0	T	C	100.0%	306.1 / NA	85	F563S (TTC→TCC)	rpoD	RNA polymerase, sigma 70 (sigma D) factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/51);  total (34/51)

ATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGT  >  CP009273/3207958‑3208222                                                                                                                                        |                                                                                                                                  aTCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCg                                                                                                                                 <  1:5094941/138‑1 (MQ=255) aTCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCg                                                                                                                                 <  1:4671949/138‑1 (MQ=255)   cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTa                                                                                                                              <  1:2895076/139‑1 (MQ=255)   cTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTa                                                                                                                              <  1:5038651/139‑1 (MQ=255)    tGGGGGATTTCATCGAGGACACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTAt                                                                                                                             <  1:3489630/139‑1 (MQ=255)     gggggATTTCATCGAGGATCCCCCCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATc                                                                                                                            <  1:2183900/139‑1 (MQ=255)         gATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGat                                                                                                                         <  1:2391935/138‑1 (MQ=255)          aTTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGata                                                                                                                        <  1:2242175/138‑1 (MQ=255)           tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATg                                                                                                                      >  1:4206338/1‑139 (MQ=255)           tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATg                                                                                                                      <  1:3643620/139‑1 (MQ=255)           tttCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATg                                                                                                                      >  1:1326160/1‑139 (MQ=255)             tCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATc                                                                                                                            <  1:778089/131‑1 (MQ=255)             tCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGaa                                                                                                                    <  1:69129/139‑1 (MQ=255)              cATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGAGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCg                                                                                                                                 <  1:2253680/125‑1 (MQ=255)                      aTACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTa                                                                                                                              <  1:842056/120‑1 (MQ=255)                       tACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAaca                                                                                                                  >  1:3360114/1‑131 (MQ=255)                       tACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTa                                                                                                           <  1:728450/138‑1 (MQ=255)                       tACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTa                                                                                                           <  1:1219968/138‑1 (MQ=255)                        accaccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTac                                                                                                          >  1:347983/1‑138 (MQ=255)                         ccccccTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTacac                                                                                                        <  1:1780894/136‑1 (MQ=255)                         ccaccCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTacac                                                                                                        <  1:4880938/139‑1 (MQ=255)                                cGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGaa                                                                                                                    >  1:3424006/1‑120 (MQ=255)                                     tGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGaagaag                                                                                             <  1:978076/138‑1 (MQ=255)                                       ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                                                                                          >  1:712628/1‑139 (MQ=255)                                       ccGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTgg                                                                                          >  1:1578443/1‑139 (MQ=255)                                          cTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAaca                                                                                                                  <  1:1018537/112‑1 (MQ=255)                                            ggATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                                                                                     <  1:3116811/139‑1 (MQ=255)                                             gATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGGATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAAc                                                                                    >  1:802691/1‑139 (MQ=255)                                                tCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt                                                                                 <  1:3996194/139‑1 (MQ=255)                                                  tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGat                                                                                                                         >  1:236596/1‑97 (MQ=255)                                                  tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTTGGTAAACAGTTc                                                                               >  1:1523754/1‑139 (MQ=255)                                                  tGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc                                                                               <  1:3744574/139‑1 (MQ=255)                                                    cGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTacac                                                                                                        >  1:3600262/1‑112 (MQ=255)                                                         accGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTa                                                                        <  1:4798932/139‑1 (MQ=255)                                                                  cTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGa                                                                 <  1:2551320/137‑1 (MQ=255)                                                                  cTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGa                                                                 <  1:4506826/137‑1 (MQ=255)                                                                  cTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAAc                                                               <  1:3181730/139‑1 (MQ=255)                                                                  cTGCGGGCGGGCACGCACGACGTTGTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTTTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGa                                                                 <  1:2396618/137‑1 (MQ=255)                                                                     cgtgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCGGCGAACGTa                                                            >  1:4975625/1‑139 (MQ=255)                                                                     cgtgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTa                                                            >  1:2331329/1‑139 (MQ=255)                                                                      gtgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTa                                                            <  1:1625701/138‑1 (MQ=255)                                                                         cgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCg                                                        >  1:2286713/1‑139 (MQ=255)                                                                         cgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGAAAACAGTTCGACGTTACCCGCGAACGTATCCg                                                        >  1:4210308/1‑139 (MQ=255)                                                                           gCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTc                                                      >  1:599435/1‑139 (MQ=255)                                                                            cAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATc                                                                                                                            <  1:4308435/68‑1 (MQ=255)                                                                             aaCGCACGACGGGCTGGCTGGCCTGACCGCGCCTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCa                                                     <  1:4910436/138‑1 (MQ=255)                                                                                gCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATc                                                                                                                            <  1:4889326/64‑1 (MQ=255)                                                                                gCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATc                                                 <  1:3384693/139‑1 (MQ=255)                                                                                    gacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATcgaa                                              >  1:3107650/1‑138 (MQ=255)                                                                                      cgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAaca                                                                                                                  >  1:1728054/1‑68 (MQ=255)                                                                                      cgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaaga                                                                                               <  1:4214307/87‑1 (MQ=255)                                                                                       gTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaaga                                                                                               <  1:3451879/86‑1 (MQ=255)                                                                                        tGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaaga                                                                                               <  1:2750488/85‑1 (MQ=255)                                                                                          ctggctggCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGCTATGAaca                                                                                                                  >  1:2310162/1‑64 (MQ=255)                                                                                           tggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgc                                      <  1:3294753/139‑1 (MQ=255)                                                                                            ggctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGgcg                                     <  1:4271487/139‑1 (MQ=255)                                                                                                ggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                                                                                     >  1:1633690/1‑87 (MQ=255)                                                                                                 gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                                                                                     >  1:869964/1‑86 (MQ=255)                                                                                                  ccTGGCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGaagaag                                                                                             <  1:2452993/77‑1 (MQ=255)                                                                                                  ccTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaaa                                                                                     >  1:167580/1‑85 (MQ=255)                                                                                                  ccTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCcg                                                                    <  1:1153458/102‑1 (MQ=255)                                                                                                       ccGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTa                                                                                                                              >  1:4025110/1‑39 (MQ=255)                                                                                                           gcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCg                       <  1:4234266/138‑1 (MQ=255)                                                                                                           gcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCg                       >  1:869827/1‑138 (MQ=255)                                                                                                           gcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGATATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTGCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGt                      <  1:2676978/139‑1 (MQ=255)                                                                                                            cgTGCAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACa                                                                                   >  1:5031905/1‑77 (MQ=255)                                                                                                            cgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATc                                                          >  1:3734963/1‑102 (MQ=255)                                                                                                                 aGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTa                                                            <  1:935546/95‑1 (MQ=255)                                                                                                                    aaaaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTacac                                                                                                        <  1:760379/48‑1 (MQ=255)                                                                                                                    aaaaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGa                                                                 <  1:1546142/87‑1 (MQ=255)                                                                                                                    aaaaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAg              <  1:4115442/138‑1 (MQ=255)                                                                                                                      aaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCaaa                            <  1:3924949/122‑1 (MQ=255)                                                                                                                         ttCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAg              <  1:3411036/133‑1 (MQ=255)                                                                                                                           cTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGAt                                                  >  1:3517358/1‑95 (MQ=255)                                                                                                                           cTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTg      <  1:1919342/139‑1 (MQ=255)                                                                                                                            tGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGa     >  1:1808847/1‑139 (MQ=255)                                                                                                                              cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaagaa                                                                                              >  1:3342433/1‑48 (MQ=255)                                                                                                                              cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt                                                                                            <  1:383667/50‑1 (MQ=255)                                                                                                                              cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGa                                                                 <  1:654114/77‑1 (MQ=255)                                                                                                                              cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGt                                                       >  1:4126925/1‑87 (MQ=255)                                                                                                                              cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAg   >  1:953628/1‑139 (MQ=255)                                                                                                                               gTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAg   <  1:3431628/138‑1 (MQ=255)                                                                                                                                tATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCAcc                  >  1:1343748/1‑122 (MQ=255)                                                                                                                                tATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGt  >  1:2074247/1‑138 (MQ=255)                                                                                                                                   gCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGaa    >  1:829073/1‑133 (MQ=255)                                                                                                                                        |                                                                                                                                  ATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCGACCACCGAAAGCCTGCGTGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGCTGCGCAAACTGCGTCACCCGAGCCGTTCTGAAGT  >  CP009273/3207958‑3208222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑