Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,547,518	G→A	E359K (GAA→AAA)	malT →	mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,547,518	0	G	A	100.0%	192.1 / NA	59	E359K (GAA→AAA)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

ACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCC  >  CP009273/3547383‑3547645                                                                                                                                        |                                                                                                                                aCCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCaaa                                                                                                                               >  1:4290702/1‑138 (MQ=255) aCCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCaaa                                                                                                                               >  1:172743/1‑138 (MQ=255)     gCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAAt                                                                                                                          >  1:560252/1‑139 (MQ=255)      cGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAAt                                                                                                                          >  1:4692105/1‑138 (MQ=255)          tGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATtcatc                                                                                                                     >  1:3689443/1‑139 (MQ=255)               ccgCTATCACCCGCTGTTTGGTACCTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATgcg                                                                                                                <  1:4245696/137‑1 (MQ=255)               cTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATgc                                                                                                                 >  1:2184731/1‑138 (MQ=255)                     tCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcg                                                                                                          >  1:2446553/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCATCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCAGCATGCTCTtgcggc                                                                                                        >  1:1783360/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGCTTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        >  1:1298155/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        >  1:3645160/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        >  1:3008692/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        >  1:341961/1‑139 (MQ=255)                       aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        >  1:2526699/1‑139 (MQ=255)                          cGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAgg                                                                                                     <  1:4418294/139‑1 (MQ=255)                            cTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCg                                                                                                   >  1:2656646/1‑139 (MQ=255)                            cTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCg                                                                                                   >  1:2339307/1‑139 (MQ=255)                                ttGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATg                                                                                                <  1:3142592/138‑1 (MQ=255)                                      aCTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc                                                                                                        <  1:2944062/124‑1 (MQ=255)                                       cTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCTATGCGCTGAtg                                                                                        <  1:1918581/139‑1 (MQ=255)                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGTGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgct                                                                                      <  1:1654387/139‑1 (MQ=255)                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgct                                                                                      <  1:3361552/139‑1 (MQ=255)                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAt                                                                                         >  1:2314730/1‑136 (MQ=255)                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAt                                                                                         >  1:3061719/1‑136 (MQ=255)                                         tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAt                                                                                         >  1:1377339/1‑136 (MQ=255)                                            tGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcg                                                                                   <  1:1740661/139‑1 (MQ=255)                                            tGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcg                                                                                   <  1:3879433/139‑1 (MQ=255)                                             gcgcCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc                                                                                  <  1:3448479/139‑1 (MQ=255)                                                ccAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcg                                                                                              >  1:361447/1‑124 (MQ=255)                                                 cAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGata                                                                              <  1:496893/139‑1 (MQ=255)                                                  aGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGatat                                                                             >  1:1050240/1‑139 (MQ=255)                                                    cgcTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGatat                                                                             >  1:2474761/1‑137 (MQ=255)                                                     gcTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATAtt                                                                            <  1:3802986/137‑1 (MQ=255)                                                              gggAACTGTCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAt                                                                   <  1:3848985/137‑1 (MQ=255)                                                              gggAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAt                                                                   <  1:2755642/137‑1 (MQ=255)                                                                   cTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGccc                                                            <  1:2112485/139‑2 (MQ=255)                                                                     ggcggcGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTgg                                                          >  1:3548106/1‑139 (MQ=255)                                                                       cggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGa                                                         <  1:73575/138‑1 (MQ=255)                                                                       cggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGa                                                         <  1:1710294/138‑1 (MQ=255)                                                                       cggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGa                                                         <  1:1287423/138‑1 (MQ=255)                                                                        ggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAg                                                        <  1:980619/138‑1 (MQ=255)                                                                             aGCTGCCGGAAATCCCCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGGAGGCGATTCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGt                                                   <  1:426189/138‑1 (MQ=255)                                                                                tGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCa                                                >  1:958396/1‑138 (MQ=255)                                                                                tGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCa                                                >  1:4531021/1‑138 (MQ=255)                                                                                  ccGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCa                                                                 <  1:685762/119‑1 (MQ=255)                                                                                      aaaTCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCagcaaagcaa                                                                                                                           <  1:3116674/57‑1 (MQ=255)                                                                                        aTCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGa                                                                                                  <  1:1268194/80‑1 (MQ=255)                                                                                            aCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGATGCGCGATATTCTGCTTAATCACGCCTGGAGt                                                       >  1:3267930/1‑119 (MQ=255)                                                                                              cGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGt                                 <  1:383132/139‑1 (MQ=255)                                                                                                tGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATgc                                                                                                                 >  1:534299/1‑57 (MQ=255)                                                                                                tGCCGCCGCAGAAAGCTGGATGGCCCAGGGAATTCACAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGCTGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCCACCATAGCGAACTGTc                                >  1:2927438/1‑138 (MQ=255)                                                                                                  ccgccgCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtg                                                                                        >  1:3849518/1‑80 (MQ=255)                                                                                                       gcAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTgg                        <  1:2532603/139‑1 (MQ=255)                                                                                                            aaGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAagag                   <  1:4764193/139‑1 (MQ=255)                                                                                                            aaGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAag                     <  1:5105506/137‑1 (MQ=255)                                                                                                                    tGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTa            <  1:2413387/138‑1 (MQ=255)                                                                                                                       cccAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAgg         >  1:4992369/1‑138 (MQ=255)                                                                                                                       cccAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGAGGCGCTGATGCTGCGCGATATTCTGCTTAATCGCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAgg         <  1:1951042/138‑1 (MQ=255)                                                                                                                         cAGTGATTTCCGAGGAAAGCAATTCACTATGCGCTGGCGGTAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCTCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGccc      <  1:4363653/139‑1 (MQ=255)                                                                                                                         cAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGc        >  1:485444/1‑137 (MQ=255)                                                                                                                              aTTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCGCGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGcc  <  1:4918401/138‑1 (MQ=255)                                                                                                                                 tCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGACGAGTCGCTTAAgg         >  1:1971667/1‑128 (MQ=255)                                                                                                                                        |                                                                                                                                ACCGGCGAGTGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTTAAGGCCCTGCC  >  CP009273/3547383‑3547645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑