Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273173,6060GA23.1% 137.9 / 14.3 65S169F (TCC→TTC) yadSUPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (29/21);  new base A (0/15);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 4.07e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAG  >  CP009273/173468‑173735
                                                                                                                                          |                                                                                                                                 
aTCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg                                                                                                                                   >  1:3871840/1‑139 (MQ=255)
 tCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg                                                                                                                                   <  1:3526401/138‑1 (MQ=255)
      cacaTCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAt                                                                                                                              <  1:1695103/138‑1 (MQ=255)
           cttctCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGCGGTACGGAAAATGTGtaa                                                                                                                        >  1:3035731/1‑139 (MQ=255)
                 tctTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGcc                                                                                                                  <  1:1827341/139‑1 (MQ=255)
                    tCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTa                                                                                                               <  1:3752380/139‑1 (MQ=255)
                     cAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTa                                                                                                               >  1:3997568/1‑138 (MQ=255)
                     cAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTa                                                                                                               >  1:1137572/1‑138 (MQ=255)
                      aaCGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGc                                                                                                             <  1:1340606/139‑1 (MQ=255)
                      aaCGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGc                                                                                                             <  1:3459257/139‑1 (MQ=255)
                      aaCGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGc                                                                                                             <  1:3309188/139‑1 (MQ=255)
                        cGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGt                                                                                                           >  1:191765/1‑139 (MQ=255)
                             aTTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACa                                                                                                      >  1:1797659/1‑139 (MQ=255)
                               gctcATCCAGCGCAAACGCCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCCCGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGa                                                                                                                                  <  1:3842583/108‑1 (MQ=255)
                                  cccccAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATGCCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTTTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAcc                                                                                                 <  1:2552411/136‑1 (MQ=255)
                                  cacccAGCGCAACCGTCGGTAGCTTAAGATGCCAACGAATGGCCGCCAGCCGAATCAATAGCGTCACGCCCATGCCCATCATACTGGCTGTTTCCAGTGGTACGAAAAAGGTGTAATAAGCCGTAGCGGGGACAATAcc                                                                                                 <  1:4214980/136‑1 (MQ=255)
                                      cAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCAAATCAATAGCGTCACGCCCATGCCCATCATACTGGCTGTTTCAAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccgccg                                                                                             <  1:1963956/139‑1 (MQ=255)
                                         cgcAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCCATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTCCGGAAAATGTGtaa                                                                                                                        >  1:1672071/1‑109 (MQ=255)
                                         cgcAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGata                                                                                          >  1:2079474/1‑139 (MQ=255)
                                         cgcAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGata                                                                                          >  1:4180174/1‑139 (MQ=255)
                                           aaatcGTCGGTAGCTTAAGATCCCACCGAATCGCCGCCAGCCAAATCAATAGCGTCACGACCATGCCCATCATCCTGGCTGTTTCCAGTGGTACGAAAAATGTGTAATACGCCGTAGCGTGGACAATCCCGCCGataat                                                                                        <  1:4043607/135‑1 (MQ=255)
                                                gtgggTAGCTTAAAATGCCAACAAATAGCCGCCAGCCGAATCAATACCGTCACGCCCATGCCCATCATACTGGCTGTTCCCAGGGGTACTGAAAATGTGTAATAGGCCGTAGCGTGGAAAATACCGCCGATAATACAgg                                                                                   <  1:1668189/136‑1 (MQ=255)
                                                gTCGGTAGCTGAGGAGGCAACCAAATAGCCGCCAGCCGAATCAATGGCGTCCCGACCATGCCAAGCATCCTGGCTGTTCCCAGGGGTACGAAAAATGGGTAATAAGCGGGGGCGGGGAAAATCCCGCCGATAATACAgg                                                                                   <  1:3850359/139‑1 (MQ=255)
                                                     tAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGcc                                                                                                                  <  1:711090/103‑1 (MQ=255)
                                                        cTTAAGAGGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTg                                                                                                          <  1:3343750/108‑1 (MQ=255)
                                                         ttAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGa                                                                                                                                  <  1:2303969/83‑1 (MQ=255)
                                                         ttAAGATGCCAACGAAAGCCCCCCAGCCAAATAAAGAGCGTCCCGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGAAAAAGGTGTAATAGGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCg                                                                           <  1:4114167/138‑1 (MQ=255)
                                                            aGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaa                                                                                                                                 >  1:3007403/1‑81 (MQ=255)
                                                               tGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGa                                                                                                        >  1:2883359/1‑103 (MQ=255)
                                                                 ccAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGAtt                                                                     >  1:3971174/1‑136 (MQ=255)
                                                                 ccAACATATCGCCGCCGCCCGAATCAAAGCCGACACGCCCAGGCCCACCTAGCTGGCGTTTCCCAGTGGTCGAAAAAAGGTTTAATAGCCCGTAGCTTGGCCAAAACCGCCGATAATACAGGCAGTTGCGTAGATTTCt                                                                  <  1:3332142/139‑1 (MQ=255)
                                                                  cAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATAccg                                                                                                >  1:1172227/1‑108 (MQ=255)
                                                                  cAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACGGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCt                                                                  <  1:1701391/138‑1 (MQ=255)
                                                                   aaCGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGtaa                                                                                                                        >  1:130845/1‑83 (MQ=255)
                                                                        aTAGCCGCCACCCGAATCATTAGCGTCCCGGCCATGCCCATCATATTGGCTTTTTCCAGTGGTACGGAAATTGTGTAATACGCCGTAGCTTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTa                                                           <  1:863677/139‑1 (MQ=255)
                                                                                cAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGataa                                                                                         >  1:746238/1‑101 (MQ=255)
                                                                                   ccGAATCAATAGCGTCACGCCCATCCCCATCATACTGGCTTTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGCCAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCt                                                                  <  1:692176/121‑1 (MQ=255)
                                                                                    agAATCAATGGCGTCACGACCATGCCCATCATCCTGGTTGTTTCCAGTGGTCGGAAAAATGTGTAAAAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCAt                                                    <  1:1589419/133‑1 (MQ=255)
                                                                                       aTCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaaa                                                                                                                                <  1:46566/55‑1 (MQ=255)
                                                                                             aGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTaaaa                                                        >  1:2864472/1‑121 (MQ=255)
                                                                                              gCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTcg                                          >  1:3760135/1‑134 (MQ=255)
                                                                                                gacACGACCAAGCCCATCATACTGGTTGTTTCAAGTGGTCCGAAAAATGTGTAATAAGCCGTCGGGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTg                                                                 <  1:1970100/107‑1 (MQ=255)
                                                                                                gTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGAAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGc                                     <  1:1073915/137‑1 (MQ=255)
                                                                                                 tCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGtaata                                                                                                                      >  1:2219831/1‑55 (MQ=255)
                                                                                                 tCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGta                                                                                                                         <  1:3317215/52‑1 (MQ=255)
                                                                                                 tCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAg                                  >  1:3110766/1‑139 (MQ=255)
                                                                                                 tCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGGAAAATCATGGGGATTTCGCGGGCCAg                                  >  1:1622799/1‑139 (MQ=255)
                                                                                                  cACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGa                                 >  1:1378195/1‑139 (MQ=255)
                                                                                                  cACGACCATGCCCAGCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAg                                  <  1:969015/138‑1 (MQ=255)
                                                                                                   aCGCCCATGCCCATCATACGGGCTTTTTCCAGGGGGAGGAAAAAGGTGTAAAAGGCCGTGGCGGGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGaa                                <  1:4249163/139‑1 (MQ=255)
                                                                                                      acaatccccATCATACTGGCTTTTTCAGGTGGTACAAAAAATTTATAAAAAGCCGTAGCGTGAAAAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACAt                             <  1:3549331/133‑1 (MQ=255)
                                                                                                          tGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACg                         >  1:593267/1‑139 (MQ=255)
                                                                                                          tGCCCATCATACTGGCTGTTTCCAGTGGCACGGAAAATGTGTAATAAGCCGTCGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAAt                                                       >  1:4139968/1‑109 (MQ=255)
                                                                                                           gCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTa                                                                                                               >  1:1145620/1‑52 (MQ=255)
                                                                                                              ctccatACTGGCTTTTTCCAGTGGTACGAAAAATGTGTAATAAGCCGTGGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTaaa                                                         <  1:2308538/100‑1 (MQ=255)
                                                                                                                 catACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGAttt                                                                    >  1:1714671/1‑89 (MQ=255)
                                                                                                                  atACTGGCTTTTTCCAGTGGTACGAAAAAGGGTTAAAAAGCCGTAGCGTGAAAAAAACCGCCGATAATACAGGCAGTTGCGTAGATTTCTg                                                                 <  1:3287682/91‑1 (MQ=255)
                                                                                                                      tGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATCATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGGCAGAACATCACGAATGATCccgcc             >  1:3688160/1‑139 (MQ=255)
                                                                                                                      tGGCTGTTTCCAGGGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTAGCGGGCCAGAACATCACGAATGATCccgcc             >  1:2657862/1‑139 (MQ=255)
                                                                                                                       ggCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCccgcc             >  1:702366/1‑138 (MQ=255)
                                                                                                                        gCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGa                                               >  1:135422/1‑103 (MQ=255)
                                                                                                                          tttttCCGGTGGTGCGAAAAAGGTGTAATAAGCCGAAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgccgcc          <  1:485245/136‑1 (MQ=255)
                                                                                                                          tttttCCGGTGGTCCGGAAAATTTTTAATAACCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgccgcc          <  1:1800916/136‑1 (MQ=255)
                                                                                                                          tGTTCCCAGGGGTCCGGAAACTGTGAAAAAGGCGGTGGCGTGAAAAATCCCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCgccgcc          <  1:91464/138‑1 (MQ=255)
                                                                                                                            tttCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAAt                                                       >  1:1116028/1‑91 (MQ=255)
                                                                                                                                cAGTGGTAGGAAAAATGTGAAAAAAGCCGTGCGGTGAAAAAACCCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCa   <  1:2912270/139‑1 (MQ=255)
                                                                                                                                    ggTACGAAAAATGTGTAATAAGCGGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAg  <  1:108936/136‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                 
ATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTGTAATAAGCCGTAGCGTGGACAATACCGCCGATAATACAGGCAGTTGCGTAGATTTCTGTACGTAAAATCATGGGGATTTCGCGGGCCAGAACATCACGAATGATCCCGCCGCCAACGCCAG  >  CP009273/173468‑173735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑