| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,933,885 | G→T | L30I (CTT→ATT) | hsrA ← | putative multidrug or homocysteine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,933,885 | 0 | G | T | 90.0% | 89.5 / ‑2.4 | 42 | L30I (CTT→ATT) | hsrA | putative multidrug or homocysteine efflux system |
| Reads supporting (aligned to +/- strand): ref base G (2/0); major base T (21/15); minor base A (1/3); total (24/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATCACTTATCAGGT > CP009273/3933751‑3934007 | aCCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTGTTAataata > 1:2251919/1‑139 (MQ=255)aCCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAataata < 1:2107386/139‑1 (MQ=255) gATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATc < 1:2878104/139‑1 (MQ=255) aTCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCa > 1:345268/1‑139 (MQ=255) tCGGCTAGCCATCCGCTTACCGGAATAAGCATCGACACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGAAAGGCGGGATTAATAATAGTGGCATCaa > 1:1058520/1‑139 (MQ=255) cTAGCCCTCCGCTTACCGGAATAAGCAGCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTg < 1:3230269/138‑1 (MQ=255) tAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGc < 1:921201/138‑1 (MQ=255) cATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCa < 1:766311/139‑1 (MQ=255) tACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTg > 1:657762/1‑115 (MQ=255) tACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAAAATAGTGGGATCAAGGGCCTGCATGaagaag > 1:4001621/1‑139 (MQ=255) aCCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGaagaag > 1:997636/1‑138 (MQ=255) aaTAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATc > 1:1966399/1‑139 (MQ=255) aaTAAGCATCGCCACCGACAGCGGATAACTGATGATGTCTGATTGCATCGCGGGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCGAGAAGAAGGCCaac > 1:3013953/1‑137 (MQ=255) aTCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATtaataa > 1:583617/1‑97 (MQ=255) gCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAaaaaaa > 1:2573891/1‑94 (MQ=255) gCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATcc > 1:417158/1‑139 (MQ=255) cGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGc > 1:1457387/1‑139 (MQ=255) tCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCaa > 1:3158032/1‑139 (MQ=255) atgatgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAAACCGCCATACTgc > 1:2885728/1‑139 (MQ=255) atgatgGCTGATAGAATCGCGAGAGTAGAACGATTCAGGCTATGGGCGATTGCGGGTAAGGCGGTATTAATAATAGGGGCGTCGAGTGCGTGCCTGAAAAAGGCCATCGCCGCGATCCACAGGAAACCCGCCCtgctgc > 1:4076456/1‑139 (MQ=255) tgatgGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATGGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCGCGGCAAACCCGCCATACTgcg < 1:1503087/139‑1 (MQ=255) tgaggGCTGATTGCATCGGGAGAGGAGAACGATTAAGGCTATGAGCGTTTGGGGGTAGGGGGGTATTAAAAATGGGGGCATCAAGTCCCTGCAGGAAGAAGGCCATCGCCGCGATCCCCGGAAACCCCGCCATACTgcg < 1:3169477/139‑1 (MQ=255) aTTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCttttt > 1:1665301/1‑139 (MQ=255) tCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAataata > 1:1720802/1‑59 (MQ=255) cgcgGGAGGAAAACGATTAGGGAATGGCGGGATGGGGGGAAAGGGGGTATAAATAATAGTGGCAACAAGTGCCTGCATGAAGAGGTCCACCGCCGCGACCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTc < 1:3626644/139‑1 (MQ=255) cgAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAataata > 1:7304/1‑56 (MQ=255) gagaGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAg > 1:2833597/1‑56 (MQ=255) agagGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCAAACTGCGCTTCTTTTTATCGCCCATTc > 1:3751219/1‑139 (MQ=255) aaCGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATc < 1:754663/55‑1 (MQ=255) cGATTAAGGCTAGGAGCTATTGCGGGTAAGGGGGTATTAATAATAGGGGCCTCAAGTGCCTGCATGAAGAAGCCCATCGCCGCGACCCACGGCAACCCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTg < 1:1831402/139‑1 (MQ=255) aTTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGc < 1:160877/91‑1 (MQ=255) ttAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGGCCTGTTa > 1:3411712/1‑139 (MQ=255) tAAGGCTATGGGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGCCCATCGCCGCGATCCCCGGCAACCCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTAt < 1:986172/139‑1 (MQ=255) aaGGCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATc > 1:895610/1‑139 (MQ=255) gCTATGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGc > 1:2926843/1‑55 (MQ=255) cTAGGAGCGATTGCGGGAAAGGGGTTAATAAAAAGGGGGGAACCAAGTGCCTGCATGAAGAGGCCCTTCGCCGCGACCCACGGAAACCCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGt < 1:3628361/139‑1 (MQ=255) tATGGGCGTTGGGGGGAAGGGGGGTATTAAAAATAGTGCCATCAAGTCCCGGCATGAAGAGGCCTATCGCCGCGATCCACGGAAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGt < 1:2518073/138‑1 (MQ=255) aTGAGCGATTGCGGGTAAGGCGGTTTTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTa > 1:494196/1‑139 (MQ=255) tGAGCGATTGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCa > 1:2333966/1‑91 (MQ=255) gAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATc > 1:2466343/1‑139 (MQ=255) ttGCGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCACTGTCCTGTTATCGGGTTATCACTTa > 1:4236783/1‑138 (MQ=255) ggggTAGGGGGTTATAAATAAGAGGGGAATAAGGTGCCTGCATGAAGAGGGCCACCGCCGCGACCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATCACTTATCa < 1:3417259/137‑1 (MQ=255) cGGGTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCa > 1:2145569/1‑109 (MQ=255) ggTAAGGCGGTATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGATTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATCACTTATCAGGt > 1:287999/1‑139 (MQ=255) tATTAATAATGGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGAAACCCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTc < 1:1279436/102‑1 (MQ=255) tATTAATAATATTGGCATAAAGTGCCTCCATGAAGAAGGCCATCGCCGCGATCCACGGCAACCCCGCCATACTGCGCTTCTTTTTATCGCTc < 1:2169331/92‑1 (MQ=255) tATTAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATcgccg < 1:3341424/48‑1 (MQ=255) ttAATAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGAAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATCACTTATCAGGt < 1:790201/127‑1 (MQ=255) | ACCGAAGCGATCGGCTAGCCATCCGCTTACCGGAATAAGCATCGCCACCGTCAGCGTATAACTGATGATGGCTGATTGCATCGCGAGAGGAGAACGATTAAGGCTATGAGCGATTGCGGGTAAGGCGGTATTAAGAATAGTGGCATCAAGTGCCTGCATGAAGAAGGCCATCGCCGCGATCCACGGCAAACCCGCCATACTGCGCTTCTTTTTATCGCTCATTCAATGTCCTGTTATCGGGTTATCACTTATCAGGT > CP009273/3933751‑3934007 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |