| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 4,224,841 | C→T | H386Y (CAC→TAC) | pgi → | glucosephosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,224,841 | 0 | C | T | 90.0% | 165.0 / 10.0 | 61 | H386Y (CAC→TAC) | pgi | glucosephosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (33/21); total (38/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01 | |||||||||||
TATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACTTCTTCGCCCAGACCGAAGC > CP009273/4224708‑4224974 | tatGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGAGTACCAGACTGGCCCGATTATCTGGGGTGACCCAGGCACTAACGGTCAGTAcgcg < 1:3718600/139‑1 (MQ=255) aaCCGATTCGCGGCGTACTTCCAGCAGGGCAATATGGAGCCCAACGGCACGTATGCTGACCGTGACGGTAACGTTGTGGAGTACCAGACTGGCCCGATTATCTGGGGAGAACCAGGCACTAACGGTCAGACCGCGTTCt > 1:3523087/2‑139 (MQ=255) cGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTAcc < 1:3202284/139‑1 (MQ=255) gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCa > 1:1030491/1‑139 (MQ=255) gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCa > 1:1342517/1‑139 (MQ=255) gTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGCTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTcctgt > 1:4260610/1‑134 (MQ=255) tttCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTAcaa > 1:1283798/1‑136 (MQ=255) tttCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCa > 1:3537387/1‑138 (MQ=255) cgcgGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTg < 1:1351884/139‑1 (MQ=255) gcagGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAAcc > 1:2696995/1‑139 (MQ=255) gTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGt > 1:1982685/1‑98 (MQ=255) gTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGc > 1:1412793/1‑139 (MQ=255) aaCGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGAtt > 1:1615744/1‑139 (MQ=255) aaCGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGAtt > 1:2407847/1‑139 (MQ=255) gTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCa > 1:1710745/1‑138 (MQ=255) tAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTg < 1:3257984/129‑1 (MQ=255) aGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGACCCAAAATGGGACCGTGCGATTTCCTc > 1:2364627/1‑137 (MQ=255) aGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATc > 1:491288/1‑137 (MQ=255) aTGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt < 1:1366219/137‑1 (MQ=255) aTGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt < 1:948417/137‑1 (MQ=255) ttGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTAc > 1:751278/1‑86 (MQ=255) ttGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCg > 1:192664/1‑137 (MQ=255) tGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGGCAGTACGCGt > 1:3015145/1‑80 (MQ=255) gACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATc > 1:1086277/1‑129 (MQ=255) aCCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTa > 1:3222822/1‑138 (MQ=255) ccGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt < 1:3989169/138‑1 (MQ=255) ccGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt < 1:3455110/138‑1 (MQ=255) ccGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt < 1:1051068/138‑1 (MQ=255) gtaacggtaacgTTGTGGATTACCAGACTGGCCCGATT‑TCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCAc > 1:1932385/1‑138 (MQ=255) ggtaacgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCAt < 1:2763288/138‑1 (MQ=255) aacgTTGTGGATTCCCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGCTCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAAcc < 1:3786411/139‑1 (MQ=255) aacgTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAAcc < 1:1651390/139‑1 (MQ=255) ttGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGc > 1:4039290/1‑138 (MQ=255) tgGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAAc < 1:1749016/131‑1 (MQ=255) tgGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGctct > 1:590824/1‑138 (MQ=255) tgGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGctc > 1:3821982/1‑137 (MQ=255) gATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATc < 1:3263907/72‑1 (MQ=255) aCCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCaaa < 1:387360/83‑1 (MQ=255) aCCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGatca < 1:782133/139‑1 (MQ=255) ccAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGatcat < 1:952781/139‑1 (MQ=255) gACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGTTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTAATCGCTCCGGCTATCACCCATAACCCGCTCTCTg > 1:3919363/1‑131 (MQ=255) aCTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTAt < 1:1869126/110‑1 (MQ=255) cTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGaa > 1:1092212/1‑72 (MQ=255) cccGATTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGt > 1:2560091/1‑83 (MQ=255) aTTATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAAct > 1:427246/1‑137 (MQ=255) ttATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAAc > 1:4039265/1‑110 (MQ=255) ttATCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAAct > 1:4026121/1‑136 (MQ=255) aTCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCt < 1:676371/90‑1 (MQ=255) tCTGGGGTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGatcat < 1:2344998/122‑1 (MQ=255) gTGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATAATCACCAGAAACTGCTGTCTAActt > 1:146480/1‑139 (MQ=255) gTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGAGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTaaat > 1:4305002/1‑136 (MQ=255) tGAACCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAAct > 1:2425862/1‑137 (MQ=255) gAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAACATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTCActtc < 1:3552529/138‑1 (MQ=255) aaCCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCa > 1:2848685/1‑90 (MQ=255) aaCCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAActtct > 1:2278600/1‑138 (MQ=255) aCCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAAc > 1:171920/1‑122 (MQ=255) aCCAGGCACTAACGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACttcttc > 1:3585894/1‑139 (MQ=255) aCGGTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACTTCTTCGCCCAGACCGa > 1:1255449/1‑139 (MQ=255) ggTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAAc < 1:2020247/84‑1 (MQ=255) gTCAGTACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCg < 1:59948/50‑1 (MQ=255) gTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGCACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACTTCTTCGCCCAGACCGAAgc > 1:819173/1‑139 (MQ=255) | TATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACTTCTTCGCCCAGACCGAAGC > CP009273/4224708‑4224974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |