| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 1,782,894 | C→A | A68E (GCA→GAA) | aroH → | 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,782,894 | 0 | C | A | 90.5% | 210.0 / 15.4 | 74 | A68E (GCA→GAA) | aroH | 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base A (36/31); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCCCGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGAT > CP009273/1782766‑1783024 | tCCCGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGaaa < 1:308740/131‑1 (MQ=255) cccGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTAc > 1:3746090/1‑138 (MQ=255) tAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAAAACTGAATGGGGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCa > 1:838031/1‑138 (MQ=255) aCGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCAccc > 1:2790349/1‑139 (MQ=255) aCGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCAccc > 1:2838901/1‑139 (MQ=255) cGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCg > 1:3752072/1‑139 (MQ=255) ccTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGc > 1:2481616/1‑131 (MQ=255) ccccATGTCCCCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTgc < 1:2098114/138‑1 (MQ=255) ccccATACCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTgc < 1:3646643/138‑1 (MQ=255) ccccGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccag < 1:3421522/137‑1 (MQ=255) cctacccccGCCGCAGAATTGAAAAAATACTGAATGGCGAAGATAAGCGCCTGTTTGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagta < 1:2356609/133‑1 (MQ=255) gACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtac > 1:1084038/1‑138 (MQ=255) aCTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtacca > 1:1584672/1‑139 (MQ=255) tCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGGCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtaccag > 1:3525339/1‑138 (MQ=255) cccGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTc > 1:391595/1‑139 (MQ=255) aaTTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCCTTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTg < 1:4137689/112‑1 (MQ=255) aTTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGaaa < 1:488820/139‑1 (MQ=255) aTTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGaa < 1:2564318/138‑1 (MQ=255) aaaaaaTACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGaaa < 1:3672190/78‑1 (MQ=255) tACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCg < 1:3044563/88‑1 (MQ=255) gAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGc > 1:1501318/1‑78 (MQ=255) gAATGGTGAAGATAAGCGACTGTGGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCa > 1:1093375/1‑137 (MQ=255) aTGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTa < 1:1646676/139‑1 (MQ=255) gTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac < 1:2505215/98‑1 (MQ=255) gTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATg < 1:351730/128‑1 (MQ=255) tGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTc < 1:1759680/87‑1 (MQ=255) tGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCg > 1:872529/1‑88 (MQ=255) aaGATAAGCGCCTGTTGGTCAGTATTGGCCCTTGCTCGATCCTCGATCTCAACGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTgcg < 1:4342631/91‑1 (MQ=255) aaGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGaaaaa > 1:1698663/1‑138 (MQ=255) aGCGACTGTTGGTCATTATTGGCCCCTACTCGATCCTCGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATg < 1:1545768/119‑1 (MQ=255) gCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtaccagt > 1:332384/1‑98 (MQ=255) gCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTAtt > 1:2524522/1‑128 (MQ=255) cGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac > 1:3930007/1‑87 (MQ=255) cGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCAc > 1:102288/1‑139 (MQ=255) gaatGTTGGTCAATATTGGCCCCTGATCGAGCCACGATCGCCCCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGCCCCGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCa < 1:3010096/134‑1 (MQ=255) gACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCAc < 1:3513448/138‑1 (MQ=255) gACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACg < 1:3921462/139‑1 (MQ=255) aCTGTTGGGCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtac > 1:2173145/1‑91 (MQ=255) tGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGa > 1:1131234/1‑137 (MQ=255) tGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGa > 1:31445/1‑137 (MQ=255) tGTTGGTCATTAATGGCCCCTGCTCGATCCACGATCTCACCGCCGCAATGGAGTACGCCACCCGTCTGCAGTc < 1:3774902/73‑1 (MQ=255) ttGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAacc < 1:846021/82‑1 (MQ=255) ggTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACtgtt < 1:2296872/139‑1 (MQ=255) ggTCATTATTGGCCCCTACTCGATCCACGATCTCACCGATGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCTGTACCAGTCACGGCTGGAAAGCGTAATGCGCACCTAtt > 1:3716546/1‑119 (MQ=255) agacaggCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCgg < 1:2570310/134‑1 (MQ=255) tattGGCCCCTGCTCGATCCACGATCTCACCGCTGaaa < 1:3187315/38‑1 (MQ=255) tattGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac > 1:1604205/1‑73 (MQ=255) tattGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGt < 1:1098869/62‑1 (MQ=255) tattGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac < 1:210096/73‑1 (MQ=255) ttGGCCCCTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTc > 1:3018059/1‑82 (MQ=255) ggcccTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGaa < 1:3988306/137‑1 (MQ=255) gcccTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGaaa > 1:1818068/2‑139 (MQ=255) ccccTGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGaa < 1:2530449/138‑1 (MQ=255) tGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGc > 1:1015502/1‑38 (MQ=255) tGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCaa > 1:3270550/1‑62 (MQ=255) tGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtaccagt > 1:2383114/1‑73 (MQ=255) tGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAgg > 1:3521019/1‑137 (MQ=255) tGCTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAgg > 1:2409876/1‑137 (MQ=255) cTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTa < 1:2500327/88‑1 (MQ=255) cTCGATCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTa > 1:357666/1‑139 (MQ=255) aTCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGa > 1:397597/1‑132 (MQ=255) tCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac < 1:2031878/56‑1 (MQ=255) tCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTCCCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGa < 1:1874051/126‑1 (MQ=255) tCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCg < 1:512301/81‑1 (MQ=255) tCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATgcg < 1:3786025/88‑1 (MQ=255) tCCACGATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAAtctc < 1:2892633/139‑1 (MQ=255) gATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCt > 1:327484/1‑88 (MQ=255) gATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAac < 1:2189808/51‑1 (MQ=255) gATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATc < 1:3198115/139‑1 (MQ=255) gATCTCACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATc < 1:617940/139‑1 (MQ=255) cACCGCTGAAATGGAGTACGCTACCCGTCCGCAGTCGCTGCGCAACCAGTACCAGTCTCGTCTGGAAATCGTAATGAGCACCTATTTTGAAAAACCAGGAACTGTTGTCGGCGGGAAATGACTAAt > 1:4044177/1‑126 (MQ=255) cACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtaccagt > 1:4201641/1‑56 (MQ=255) cACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCAc > 1:2684886/1‑81 (MQ=255) cACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTAtttt > 1:1615359/1‑88 (MQ=255) cACCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGAt > 1:1026317/1‑139 (MQ=255) aCCGCTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCa < 1:3928317/43‑1 (MQ=255) cTGAAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAaccagtaccagt > 1:16460/1‑51 (MQ=255) | TCCCGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGCAGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGCTCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGAT > CP009273/1782766‑1783024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |