Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,466,500	G→A	T125I (ACC→ATC)	fusA ←	protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,466,500	0	G	A	100.0%	158.6 / NA	45	T125I (ACC→ATC)	fusA	protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/30);  total (15/30)

AACCGGGTTCGCGCCCAGACGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCG  >  CP009273/3466373‑3466632                                                                                                                                |                                                                                                                                     aaCCGGGTTCGCGCCCAGACGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTg                                                                                                                            <  1:2514446/138‑1 (MQ=255)             gcCCAGACGGGTTTTGATATGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGCCTGCGGCTGAAcacc                                                                                                                <  1:2273602/138‑1 (MQ=255)               ccAGACGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACaccacc                                                                                                             <  1:1758189/139‑1 (MQ=255)                    cGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCACTGCGCGGATCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTg                                                                                                        <  1:1446065/139‑1 (MQ=255)                    cGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCaa                                                                                                           <  1:403327/136‑1 (MQ=255)                    cGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTg                                                                                                        <  1:186182/139‑1 (MQ=255)                         tttGATCTGGTTAACAACTTTCAGGAAGGTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAg                                                                                                   <  1:1760902/139‑1 (MQ=255)                             aTCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTgcg                                                                                                      <  1:2706096/132‑1 (MQ=255)                             aTCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTaa                                                                                                >  1:1649493/1‑138 (MQ=255)                                 ggTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAt                                                                                           >  1:2358542/1‑139 (MQ=255)                                  gTTAACAACTTTCAGGCAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGGCGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGAAACCCAtt                                                                                          >  1:4114495/1‑139 (MQ=255)                                  gTTAACAACTTTCAGGAAGTTCGCACCCATGCTGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTgcg                                                                                                      <  1:3010486/127‑1 (MQ=255)                                     aacaacTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                                                                                       <  1:4213131/139‑1 (MQ=255)                                       caacTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGACCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGc                                                                                     <  1:3911674/139‑1 (MQ=255)                                       caacTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa                                                                                            >  1:533535/1‑132 (MQ=255)                                        aacTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACTCAAGGCGCGGAGCGTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCa                                                                                    <  1:3855761/139‑1 (MQ=255)                                        aacTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCa                                                                                    <  1:3882637/139‑1 (MQ=255)                                            ttCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa                                                                                            >  1:838422/1‑127 (MQ=255)                                             tCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGAc                                                                                                                              <  1:442724/92‑1 (MQ=255)                                                   aGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGTGCAGTAAACCATTACCGCACCATCGAGaa                                                                          <  1:2552990/138‑1 (MQ=255)                                                   aGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGaa                                                                          <  1:1241609/138‑1 (MQ=255)                                                           cccATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATgg                                                                 <  1:185489/139‑1 (MQ=255)                                                            ccATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGa                                                                <  1:1944564/139‑1 (MQ=255)                                                            ccATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGa                                                                <  1:4304274/139‑1 (MQ=255)                                                                    tCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCt                                                         >  1:2242499/1‑138 (MQ=255)                                                                                        aaTGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAAc                                     <  1:3820088/138‑1 (MQ=255)                                                                                          tGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTg                                  <  1:866550/139‑1 (MQ=255)                                                                                              cgGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGcccc                              >  1:1330341/1‑139 (MQ=255)                                                                                                 aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGaa                                                                          <  1:2903258/92‑1 (MQ=255)                                                                                                 aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATg                                                                  <  1:3468846/100‑1 (MQ=255)                                                                                                  aCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCgggg                          <  1:1510835/139‑1 (MQ=255)                                                                                                  aCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCggg                           <  1:636607/138‑1 (MQ=255)                                                                                                  aCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCggg                           <  1:2299342/138‑1 (MQ=255)                                                                                                    tttATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGt                                                                                                  <  1:2937970/65‑1 (MQ=255)                                                                                                        tatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCa                                                                    <  1:1871302/91‑1 (MQ=255)                                                                                                           ttgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGa                                                                >  1:731037/1‑92 (MQ=255)                                                                                                           ttgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTa                                                        >  1:1297559/1‑100 (MQ=255)                                                                                                           ttgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCgatga                 >  1:1898624/1‑139 (MQ=255)                                                                                                             gtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGatgatg               >  1:160871/1‑139 (MQ=255)                                                                                                              tttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAc                                                                                        >  1:765797/1‑65 (MQ=255)                                                                                                                  ccTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTc                                                          >  1:4041434/1‑91 (MQ=255)                                                                                                                   cTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCA‑TACCGCCCCATCGAGaa                                                                          <  1:2420527/73‑1 (MQ=255)                                                                                                                          cATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAAc                                     <  1:3848731/104‑1 (MQ=255)                                                                                                                            tACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGCGTCGATGATGTTGatgcgatgcg  >  1:3322219/1‑137 (MQ=255)                                                                                                                             aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCA‑TACCGCACCATCGAGAACACGCATGGa                                                                >  1:247569/1‑73 (MQ=255)                                                                                                                                |                                                                                                                                     AACCGGGTTCGCGCCCAGACGGGTTTTGATCTGGTTAACAACTTTCAGGAAGTTCGCACCCATGCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACGCATGGAACGTTCTACTTCGATTGTGAAGTCAACGTGCCCCGGGGTGTCGATGATGTTGATGCGATGCG  >  CP009273/3466373‑3466632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑