| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 130,787 | T→A | intergenic (+88/‑88) | acnB → / → yacL | bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase/UPF0231 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 130,787 | 0 | T | A | 90.6% | 171.0 / 3.3 | 53 | intergenic (+88/‑88) | acnB/yacL | bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase/UPF0231 family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base A (21/27); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGT > CP009273/130656‑130918 | acacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGc < 1:1908352/136‑1 (MQ=255) cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGtt < 1:2125405/139‑1 (MQ=255) cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAACCCATCGCCGTACCGCTGttttt < 1:1191801/139‑1 (MQ=255) cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGtttt > 1:852102/1‑138 (MQ=255) cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGtttt > 1:2513037/1‑138 (MQ=255) cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGtttt > 1:2494006/1‑138 (MQ=255) gagaAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTtc < 1:444396/139‑1 (MQ=255) gagaAAGCCGAGGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGtttt > 1:2268256/1‑137 (MQ=255) cGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGt < 1:64673/137‑1 (MQ=255) gATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAg > 1:244402/1‑139 (MQ=255) tGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAgg < 1:1035557/138‑1 (MQ=255) gTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGc < 1:844498/138‑1 (MQ=255) tGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCg < 1:1668367/138‑1 (MQ=255) tGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCg < 1:350429/138‑1 (MQ=255) ttttCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATa < 1:1946160/138‑1 (MQ=255) ttCCAGACTGCGGTTTAAAAAGTCAGCGCCCGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAAt < 1:2338238/138‑1 (MQ=255) cTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACAt < 1:794756/136‑1 (MQ=255) cTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACAt < 1:2275854/136‑1 (MQ=255) cGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATg > 1:914600/1‑138 (MQ=255) gTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAg < 1:1650813/106‑1 (MQ=255) cAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGAtt > 1:27892/1‑138 (MQ=255) gcgcTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGACGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATaa > 1:226371/1‑106 (MQ=255) gcATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCgg < 1:2455687/138‑1 (MQ=255) aaaCGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCa < 1:1451136/139‑1 (MQ=255) acacAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGGTTTCGGTGCATTGCCGGGAGCAGAGGa > 1:2672057/1‑139 (MQ=255) acacAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGa > 1:101131/1‑139 (MQ=255) acacAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGa > 1:1689757/1‑139 (MQ=255) acacAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGa > 1:26450/1‑139 (MQ=255) cacaATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGaa < 1:1070613/139‑1 (MQ=255) cacaATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGa < 1:945944/138‑1 (MQ=255) ccGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTa < 1:1452574/138‑1 (MQ=255) ccGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTa < 1:1248957/138‑1 (MQ=255) tGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTAcc < 1:574832/48‑1 (MQ=255) tGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCg < 1:2509413/73‑1 (MQ=255) tGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGa < 1:895758/138‑1 (MQ=255) tGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGa < 1:1525720/138‑1 (MQ=255) tCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTtc > 1:1998967/1‑48 (MQ=255) tCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACAt > 1:1086044/1‑73 (MQ=255) tCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAAtt > 1:1846482/1‑137 (MQ=255) ccGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtc < 1:1894788/46‑1 (MQ=255) ccGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCt > 1:2256757/1‑138 (MQ=255) ccGGGCATTTTTTTATTTCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGCAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCt > 1:1411265/1‑138 (MQ=255) ttttATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAgg > 1:470554/1‑46 (MQ=255) tttATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCAGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTAc < 1:2263329/139‑1 (MQ=255) tCTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGaa < 1:881615/98‑1 (MQ=255) cTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTg > 1:203132/1‑139 (MQ=255) cTAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTg > 1:536607/1‑139 (MQ=255) aaaCCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGt < 1:1627607/139‑1 (MQ=255) aaCCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTa < 1:1960767/139‑1 (MQ=255) aCCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTaa < 1:1919955/139‑1 (MQ=255) ccATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTgcg < 1:518666/118‑1 (MQ=255) tCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGt > 1:495751/1‑139 (MQ=255) cGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATgg > 1:2305314/1‑98 (MQ=255) | ACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGT > CP009273/130656‑130918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |