| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,547,518 | G→A | E359K (GAA→AAA) | malT → | mal regulon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,547,518 | 0 | G | A | 94.7% | 57.1 / ‑6.2 | 19 | E359K (GAA→AAA) | malT | mal regulon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/9); minor base C (0/1); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTT > CP009273/3547392‑3547634 | tGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATtcatc > 1:1997198/1‑139 (MQ=255)tGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATtcatc > 1:2784267/1‑139 (MQ=255) ggTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATtcatca < 1:2013058/139‑1 (MQ=255) aCCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATtcat > 1:2520559/1‑125 (MQ=255) cTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCg > 1:995540/1‑139 (MQ=255) cTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCg > 1:725307/1‑139 (MQ=255) cTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAt > 1:2006053/1‑138 (MQ=255) tCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgct < 1:201002/139‑1 (MQ=255) gcCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGcgcg < 1:2439670/138‑1 (MQ=255) cAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGata < 1:2557173/139‑1 (MQ=255) cgcTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGTTTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGatat > 1:2717409/1‑137 (MQ=255) gggAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAt < 1:698065/137‑1 (MQ=255) gggAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCa < 1:1828940/139‑1 (MQ=255) ggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCCAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGt < 1:1483754/139‑1 (MQ=255) ggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAg < 1:573061/138‑1 (MQ=255) aaaTCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAt < 1:2029466/113‑1 (MQ=255) tGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGa > 1:605354/1‑113 (MQ=255) aaGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAaga < 1:2149284/138‑1 (MQ=255) gATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCtt > 1:1137855/1‑139 (MQ=255) | TGGTTCTGCTATCACCCGCTGTTTGGTAACTTCCTGCGCCAGCGCTGCCAGTGGGAACTGGCGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAATCACGCCTGGAGTCTGTTCAACCATAGCGAACTGTCGCTGCTGGAAGAGTCGCTT > CP009273/3547392‑3547634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |