Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	155,132	C→G	R161P (CGC→CCC)	pcnB ←	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	155,132	0	C	G	100.0%	91.3 / NA	26	R161P (CGC→CCC)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/8);  total (18/8)

TTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATA  >  CP009273/155017‑155263                                                                                                                    |                                                                                                                                    ttCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCggggcg                                                                                                                                 >  1:2096760/1‑120 (MQ=255)         tACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGaa                                                                                                      >  1:2737214/1‑139 (MQ=255)                   aCGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGc                                                                                                                           <  1:236114/108‑1 (MQ=255)                     ggATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcgc                                                                                           >  1:270946/1‑138 (MQ=255)                      gATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCgggg                                                                                                                                   <  1:244358/97‑1 (MQ=255)                             ccGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCg                                                                                                                 >  1:1660537/1‑108 (MQ=255)                                tCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGt                                                                                                                         >  1:1668775/1‑97 (MQ=255)                                 ccTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGcc                                                                              >  1:1120265/1‑139 (MQ=255)                                      aGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTcg                                                                                            <  1:31491/120‑1 (MQ=255)                                        aTCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTg                                                                        >  1:1550084/1‑138 (MQ=255)                                            ttCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGGTGGAGCCGAAACTGTTGTCGCGCAGCAACATGCCGTTTTGCCcgc                                                                   >  1:2670896/1‑139 (MQ=255)                                                tGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACa                                                                                  >  1:1456163/1‑120 (MQ=255)                                                  ccgccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTggg                                                             >  1:894815/1‑139 (MQ=255)                                                   cgccAACGTAATCACGTACGGTAAACTCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATgg                                                                                                             <  1:2694796/90‑1 (MQ=255)                                                     ccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAgg                                                          >  1:1972782/1‑139 (MQ=255)                                                             aTCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAt                                                                                                   >  1:1271751/1‑90 (MQ=255)                                                             aTCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCgg                                                  >  1:1156170/1‑139 (MQ=255)                                                                    acggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTg                                            >  1:1829777/1‑138 (MQ=255)                                                                                gCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgt                               >  1:2106095/1‑139 (MQ=255)                                                                                  tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                              <  1:2447523/138‑1 (MQ=255)                                                                                  tACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtg                              <  1:1094666/138‑1 (MQ=255)                                                                                         tAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTggg                                                             <  1:1507672/100‑1 (MQ=255)                                                                                                   tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCg                                                   >  1:83064/1‑100 (MQ=255)                                                                                                   tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc            >  1:173655/1‑139 (MQ=255)                                                                                                   tGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc            <  1:125512/139‑1 (MQ=255)                                                                                                     ttGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCa           >  1:2115119/1‑138 (MQ=255)                                                                                                       gATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACt        <  1:682079/139‑1 (MQ=255)                                                                                                             gAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATa  >  1:304795/1‑139 (MQ=255)                                                                                                                    |                                                                                                                                    TTCCGGGTTACCAATCAGACGGATAACGCCGTCCTTCAGATCCTTCATGCCGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATA  >  CP009273/155017‑155263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑