| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 1,256,820 | A→G | I171T (ATC→ACC) | prs ← | phosphoribosylpyrophosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,256,820 | 0 | A | G | 92.5% | 121.9 / 0.2 | 40 | I171T (ATC→ACC) | prs | phosphoribosylpyrophosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (20/17); total (22/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCC > CP009273/1256686‑1256948 | aGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTc > 1:2689599/1‑139 (MQ=255) gTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTcc > 1:690728/1‑139 (MQ=255) aCGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGga < 1:530431/139‑1 (MQ=255) cAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACcac > 1:55421/1‑139 (MQ=255) cACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTgg > 1:1062127/1‑139 (MQ=255) ccGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAACTGGGt > 1:2297851/1‑139 (MQ=255) atGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGa < 1:1985604/139‑1 (MQ=255) gCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTACCATATCGGTATAGTTCAGAAGCTTAGCGATAGCGCGGGCACGCACAATGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGAt > 1:1194538/1‑138 (MQ=255) tCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAg < 1:1883052/139‑1 (MQ=255) cACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCa > 1:801173/1‑137 (MQ=255) cACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCa > 1:561449/1‑137 (MQ=255) ccTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCt > 1:1430038/1‑138 (MQ=255) gtgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATc < 1:283631/124‑1 (MQ=255) tgAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAAc < 1:1419677/108‑1 (MQ=255) gAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTgcagca < 1:1266536/139‑1 (MQ=255) aCGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGt < 1:5316/139‑1 (MQ=255) cGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGc > 1:1708005/1‑124 (MQ=255) gCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTggg > 1:2842533/1‑108 (MQ=255) cgTTTGTCGATGATTGCTATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCAGTCACGCACATCACCGCCGATGTCCGGAGAATCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggc < 1:2617716/138‑2 (MQ=255) ccATATCGGTATCGTTCATCAGCTGAGCGATAGCGCGGGCACGCACAACGCCGCCGATGGCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATc > 1:2626771/1‑139 (MQ=255) atatCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCa > 1:1744278/1‑138 (MQ=255) atCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCggg < 1:2089721/118‑1 (MQ=255) tCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACg > 1:1226448/1‑138 (MQ=255) ttCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAAc < 1:368206/56‑1 (MQ=255) ttCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATa > 1:665692/1‑118 (MQ=255) cTTAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCCGGATCGGGCTACCAAATACGTTATCAACCGGACCGTCGCAGAAAc > 1:2353392/1‑137 (MQ=255) ttAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCTGGc < 1:1395882/100‑1 (MQ=255) tAGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTggg > 1:1792476/1‑56 (MQ=255) aGCGATAGCGCGGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTggg < 1:413807/55‑1 (MQ=255) cgcgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATAc > 1:2818768/1‑100 (MQ=255) gcgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGtt < 1:7000/137‑1 (MQ=255) gcgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCa < 1:319939/139‑1 (MQ=255) gcgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCa < 1:2649577/139‑1 (MQ=255) gcgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCa < 1:2364764/139‑1 (MQ=255) cgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGAt > 1:1838087/1‑55 (MQ=255) cgGGCACGCACAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCa > 1:672336/1‑138 (MQ=255) gcacgcacAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCg < 1:929774/138‑1 (MQ=255) gcacAACGCCGCCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCACATACGTTATCAACCgg < 1:2633594/103‑1 (MQ=255) cgccgcCGGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATcc > 1:1864355/1‑137 (MQ=255) gccgGTGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCgaag > 1:1210441/1‑103 (MQ=255) | AGTCACGACCTGCAACGTCACCGATGATATGCATCACCTGTGAAACGTTCGCACGCGGACGACGTTTGTCGATGATTGCCATATCGGTATCGTTCAGCAGCTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCC > CP009273/1256686‑1256948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |