Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	1,783,016	G→A	D109N (GAT→AAT)	aroH →	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,783,016	0	G	A	100.0%	171.5 / NA	47	D109N (GAT→AAT)	aroH	3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)

CACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTG  >  CP009273/1782886‑1783148                                                                                                                                   |                                                                                                                                     cACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGAt                                                                                                                              >  1:2129181/1‑139 (MQ=255) cACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGAt                                                                                                                              >  1:116178/1‑139 (MQ=255) cACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGAt                                                                                                                              >  1:251420/1‑139 (MQ=255) cACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGAt                                                                                                                              >  1:2530298/1‑139 (MQ=255)            tGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGc                                                                                                                     <  1:2844157/137‑1 (MQ=255)                  aCGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTAt                                                                                                               <  1:1561630/137‑1 (MQ=255)                   cGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCgg                                                                                                            <  1:1675829/139‑1 (MQ=255)                      cACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCggg                                                                                                           >  1:1421302/1‑137 (MQ=255)                         ccGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTaaa                                                                                                       >  1:1170267/1‑138 (MQ=255)                         ccGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTaaa                                                                                                       >  1:1671102/1‑138 (MQ=255)                           gTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCa                                                                                                    <  1:291090/139‑1 (MQ=255)                           gTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCa                                                                                                    <  1:1106967/139‑1 (MQ=255)                                              ccagtaccagtCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATc                                                                                                                                   <  1:1954465/89‑1 (MQ=255)                                                  taccagtCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATAGGGTAAATCACGGTCTGGAGCTGgcg                                                                                    <  1:266630/132‑1 (MQ=255)                                                    ccagtCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTa                                                                                                                <  1:2089338/102‑1 (MQ=255)                                                       gtCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTa                                                                                                                <  1:1573007/99‑1 (MQ=255)                                                        tCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTaaa                                                                                                                         >  1:530294/1‑89 (MQ=255)                                                         cACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACtttt                                                                      <  1:915539/139‑1 (MQ=255)                                                          aCGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACtttt                                                                      >  1:106597/1‑138 (MQ=255)                                                          aCGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGACAGCTATCGGGAAAATCACGGTCTGGAGCTGGCGCGCAAATTACtttt                                                                      >  1:875551/1‑138 (MQ=255)                                                           cGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTAc                                                                    <  1:246686/139‑1 (MQ=255)                                                            ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCa                                                                                                    <  1:2577322/106‑1 (MQ=255)                                                            ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACg                                                                                                  <  1:1903398/108‑1 (MQ=255)                                                            ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACa                                                                   >  1:2339176/1‑139 (MQ=255)                                                            ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATAGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTAc                                                                          >  1:1691021/1‑132 (MQ=255)                                                              cTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAAt                                                                                                      >  1:665308/1‑102 (MQ=255)                                                               tGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTCTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAgg                                                                 <  1:775714/138‑1 (MQ=255)                                                                 gAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAAt                                                                                                      >  1:148466/1‑99 (MQ=255)                                                                  aaaTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTaaa                                                             >  1:614471/1‑139 (MQ=255)                                                                      cGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAg                                                                                          >  1:1154069/1‑106 (MQ=255)                                                                      cGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCt                                                                                        >  1:479170/1‑108 (MQ=255)                                                                      cGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAAtt                                                                            <  1:2705062/120‑1 (MQ=255)                                                                         aaTGCGCCCCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTg                                                      <  1:2593491/139‑1 (MQ=255)                                                                             cgcACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCg                                                  >  1:530356/1‑139 (MQ=255)                                                                               cACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGt                                                 >  1:859720/1‑138 (MQ=255)                                                                                aCCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACa                                                                   <  1:1770220/119‑1 (MQ=255)                                                                                aCCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAg                                                                  >  1:1282043/1‑120 (MQ=255)                                                                                   tATTTTGAAAAACCACGAACTGTTTACGGCTGGAAAGGGCTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGCGCTGGCGCGCAAATTACTTTTACAGGCAAATGATCTGGGCGTCCCa                                             >  1:2413315/1‑138 (MQ=255)                                                                                          aaaaaCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAg                                                         >  1:345913/1‑119 (MQ=255)                                                                                                cACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGtt                               <  1:1954527/139‑1 (MQ=255)                                                                                                  cGAACTGTTGTCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTcc                             <  1:1238664/139‑1 (MQ=255)                                                                                                         ttgtCGGCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTaa                                                              <  1:1631832/99‑1 (MQ=255)                                                                                                              ggCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTg                  >  1:1394112/1‑138 (MQ=255)                                                                                                              ggCTGGAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTg                  >  1:1572726/1‑138 (MQ=255)                                                                                                                   gAAAGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTggg                                                    >  1:207361/1‑99 (MQ=255)                                                                                                                    aaaGGACTAATCTCCAATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGt            <  1:1154217/138‑1 (MQ=255)                                                                                                                             aTCTCCAATCCAGATTTAACCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTg  <  1:1540759/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     CACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAACCAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTG  >  CP009273/1782886‑1783148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑