| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,547,518 | G→A | E359K (GAA→AAA) | malT → | mal regulon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,547,518 | 0 | G | A | 94.6% | 114.2 / ‑1.7 | 39 | E359K (GAA→AAA) | malT | mal regulon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (12/23); total (13/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
CGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAT > CP009273/3547453‑3547580 | cggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCaaa < 1:3911179/68‑1 (MQ=255)cggcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCaaa < 1:2850510/68‑1 (MQ=255) gcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCATGGATTTCCCAGCAAAGc < 1:3180090/68‑1 (MQ=255) gcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGc < 1:3867349/68‑1 (MQ=255) gcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGc < 1:4104619/68‑1 (MQ=255) gcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGc < 1:3573756/68‑1 (MQ=255) gcggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGc < 1:2609152/68‑1 (MQ=255) cggAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCa < 1:4767267/68‑1 (MQ=255) aTCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCCTgcgga > 1:3656088/1‑66 (MQ=255) aTCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCt > 1:2332222/1‑68 (MQ=255) tCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTg > 1:137051/1‑68 (MQ=255) ccACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTgg < 1:925471/68‑1 (MQ=255) aCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcg > 1:1433562/1‑68 (MQ=255) aCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcg > 1:350645/1‑68 (MQ=255) aCCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCAggcg > 1:478335/1‑68 (MQ=255) cGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTggcggc > 1:3435396/1‑68 (MQ=255) gcAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATgc < 1:3850154/68‑1 (MQ=255) gcAGAAAGCTGGATGGCCCAGGGAATTCCCAGCGAAGCAATTCATCATGCGCTGGCGGGAGGCGATgc > 1:2447938/1‑68 (MQ=255) cAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcg < 1:1370970/68‑1 (MQ=255) cAGAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATgcg < 1:2632198/68‑1 (MQ=255) gAAAGCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCt < 1:2610172/68‑1 (MQ=255) aaGCTGGATGGCCCAGGGATTTCCCAGCTAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGa < 1:1556777/68‑1 (MQ=255) cgCTGGGTGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAt < 1:2542370/67‑1 (MQ=255) gCTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtg > 1:8549/1‑68 (MQ=255) cTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgc > 1:2675482/1‑68 (MQ=255) cTGGATGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGAtgc < 1:793033/68‑1 (MQ=255) aTGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcg > 1:4069809/1‑68 (MQ=255) tGGCCCCGGGAGTTCCCCGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc < 1:4310997/68‑1 (MQ=255) tGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc < 1:1391569/68‑1 (MQ=255) tGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc < 1:586300/68‑1 (MQ=255) tGGCCCAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTgcgc < 1:3229925/68‑1 (MQ=255) cAGGGATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGatat < 1:2490657/68‑1 (MQ=255) ggATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCt < 1:2212221/68‑1 (MQ=255) ggATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCt < 1:351833/68‑1 (MQ=255) ggATTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCt < 1:755476/68‑1 (MQ=255) gATTTCCCAGCAAAGCAATTCATCATGCGCTGGCAGCAGGCGATGCGCTGATGCTGCGCGATAATCTg > 1:3432695/1‑68 (MQ=255) ttttCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGc < 1:4055102/67‑1 (MQ=255) aTTTCCCTGCTAAGCAATTCATCACGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGc < 1:274982/68‑1 (MQ=255) aTTTCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGc < 1:1921217/68‑1 (MQ=255) tCCCAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTa < 1:3983937/68‑1 (MQ=255) ccAGCAAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAt > 1:4518419/1‑68 (MQ=255) | CGGCGGAGCTGCCGGAAATCCACCGTGCCGCCGCAGAAAGCTGGATGGCCCAGGGATTTCCCAGCGAAGCAATTCATCATGCGCTGGCGGCAGGCGATGCGCTGATGCTGCGCGATATTCTGCTTAAT > CP009273/3547453‑3547580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |