| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 4,275,144 | C→T | intergenic (‑3/+197) | yjcH ← / ← acs | DUF485 family inner membrane protein/acetyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,275,144 | 0 | C | T | 98.2% | 189.6 / ‑4.6 | 57 | intergenic (‑3/+197) | yjcH/acs | DUF485 family inner membrane protein/acetyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (26/30); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
TTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAC > CP009273/4275079‑4275207 | tttttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAg < 1:3830927/68‑1 (MQ=255) ttCGACTAACTCACTGAAATGCGCATAGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGAtt > 1:3199960/1‑68 (MQ=255) cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct < 1:834237/68‑1 (MQ=255) cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct < 1:79444/68‑1 (MQ=255) cGACTAACACCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct < 1:3325835/68‑1 (MQ=255) cGACAAACACCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct < 1:4424507/68‑1 (MQ=255) aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCaga < 1:3649035/68‑1 (MQ=255) aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCaga < 1:3765369/68‑1 (MQ=255) aCTCCCTGTAATGCGCATTGTCTTCTATCCGCTGATATATAGTGCCATTCATCATAGCTTCTCCagag < 1:4098233/68‑1 (MQ=255) aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag < 1:3252966/68‑1 (MQ=255) aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag < 1:1708054/68‑1 (MQ=255) aCTCCCTGAAATGCGCATTGTCATCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag < 1:3903827/68‑1 (MQ=255) tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt > 1:297972/1‑68 (MQ=255) cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAAt < 1:693504/68‑1 (MQ=255) tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATg < 1:318615/68‑1 (MQ=255) tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATg < 1:2505873/68‑1 (MQ=255) gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGt > 1:1266242/1‑68 (MQ=255) aaaTGCGCATTGTCTTCTATCCGCTGATATATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa > 1:2563631/1‑68 (MQ=255) aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTATTGTa > 1:1756412/1‑68 (MQ=255) aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa < 1:213114/68‑1 (MQ=255) aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa > 1:1762436/1‑68 (MQ=255) gcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGAtt > 1:2810207/1‑68 (MQ=255) gcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGAtt > 1:3498627/1‑68 (MQ=255) aTTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt < 1:1304755/68‑1 (MQ=255) aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt > 1:2914814/1‑68 (MQ=255) ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtt < 1:1017809/68‑1 (MQ=255) tagTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGAATGtt < 1:4268155/66‑1 (MQ=255) ttctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAt > 1:3296622/1‑68 (MQ=255) tctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAtt > 1:1214398/1‑68 (MQ=255) ctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc > 1:285290/1‑68 (MQ=255) gCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc > 1:442331/1‑68 (MQ=255) gCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc < 1:923754/68‑1 (MQ=255) gCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc < 1:1097412/68‑1 (MQ=255) tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc < 1:449062/68‑1 (MQ=255) tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc < 1:1380969/68‑1 (MQ=255) tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc < 1:3602730/68‑1 (MQ=255) tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc < 1:2356512/68‑1 (MQ=255) tGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc < 1:1257893/68‑1 (MQ=255) ggATAAATAGTGCCATTCATCATAGATTCTCCAGCGGTAATGTAGGGCTTGTTTTAATTCCCGCTccc < 1:2320854/67‑1 (MQ=255) aTAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCtt < 1:2135603/68‑1 (MQ=255) aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg > 1:2859621/1‑68 (MQ=255) aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg > 1:1103960/1‑68 (MQ=255) aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCACCCTTATgg > 1:314977/1‑68 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCGTTATGGga > 1:47648/1‑68 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgggc > 1:2558272/1‑67 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga > 1:4173845/1‑68 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga < 1:1201710/68‑1 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga > 1:3350949/1‑68 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga > 1:1377883/1‑68 (MQ=255) aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga > 1:2954420/1‑68 (MQ=255) aGTGACATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga > 1:2686789/1‑68 (MQ=255) gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa > 1:4423833/1‑68 (MQ=255) ccATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg < 1:1457071/68‑1 (MQ=255) tcatcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa > 1:2969652/1‑68 (MQ=255) tcatcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa > 1:4082799/1‑68 (MQ=255) atcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc < 1:4457256/68‑1 (MQ=255) atcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc < 1:3058107/68‑1 (MQ=255) | TTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAC > CP009273/4275079‑4275207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |