Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,276,215 C→A R362L (CGC→CTC)  acs ← acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,276,2150CA93.0% 130.9 / 1.8 43R362L (CGC→CTC) acsacetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (20/20);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCC  >  CP009273/4276159‑4276274
                                                        |                                                           
gcAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTg                                                  >  1:1029023/1‑68 (MQ=255)
   gCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGt                                               <  1:2735844/68‑1 (MQ=255)
     gacgaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTgg                                             >  1:567377/1‑67 (MQ=255)
     gacgaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGc                                             <  1:4435600/68‑1 (MQ=255)
     gacgaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGc                                             <  1:2245644/68‑1 (MQ=255)
        gatcggtcggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:1541163/65‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:1079307/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:4447626/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:4006781/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:349184/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:2738666/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:2287825/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:1891970/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGt                                          <  1:1475760/68‑1 (MQ=255)
        gaACGGTCGGTGCCTTCGATCGCTTTATCGCCTGCCGCCATCAGCGCGAGGAGCGCCGTGGGTGCGGt                                          <  1:2903032/68‑1 (MQ=255)
          aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                        <  1:1999019/68‑1 (MQ=255)
          aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                        <  1:4505000/68‑1 (MQ=255)
          aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                        <  1:1399017/68‑1 (MQ=255)
          aCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGtat                                        >  1:2557560/1‑68 (MQ=255)
            ggtcggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATag                                      >  1:1428034/1‑68 (MQ=255)
            ggtcggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATag                                      <  1:1525857/68‑1 (MQ=255)
               cggtGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAgaga                                   <  1:380254/68‑1 (MQ=255)
                    ccTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATAtt                              >  1:2010471/1‑68 (MQ=255)
                        cGATCGCTTTATCGCGTTCCGGCATCAGCGCGAGGATGGCCGTGGGTGCGGTATAGTGAGTATTGTcc                          >  1:3428828/1‑68 (MQ=255)
                          aTCGCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTGTAGAGAATATTGACCTg                        >  1:749801/1‑68 (MQ=255)
                             gCTTTATCGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATg                     >  1:808435/1‑68 (MQ=255)
                                    cGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcc              >  1:1709939/1‑68 (MQ=255)
                                    cGCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcc              >  1:3305301/1‑68 (MQ=255)
                                     gCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca             >  1:4114822/1‑68 (MQ=255)
                                     gCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca             >  1:1358019/1‑68 (MQ=255)
                                     gCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca             >  1:1644960/1‑68 (MQ=255)
                                     gCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca             >  1:4073260/1‑68 (MQ=255)
                                     gCCTTCCGCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTcca             >  1:3536288/1‑68 (MQ=255)
                                            gCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCCCCACCTg      >  1:3965303/1‑68 (MQ=255)
                                            gCCATCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTg      >  1:3536226/1‑68 (MQ=255)
                                             ccATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTgc     <  1:1631999/68‑1 (MQ=255)
                                                tCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  >  1:2186256/1‑68 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  <  1:2644262/68‑1 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  >  1:3808854/1‑68 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  >  1:4366119/1‑68 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  >  1:1423142/1‑68 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  >  1:1707538/1‑68 (MQ=255)
                                                tCAGCGCGAGGATCGCCGTGGGGGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGcc  <  1:4330250/68‑1 (MQ=255)
                                                        |                                                           
GCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACCTGATGCTTGTCCACCACCTGCGCC  >  CP009273/4276159‑4276274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑