| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 4,629,517 | C→T | V203M (GTG→ATG) | arcA ← | response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,629,517 | 0 | C | T | 94.6% | 114.5 / ‑2.7 | 37 | V203M (GTG→ATG) | arcA | response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (15/20); total (15/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGC > CP009273/4629451‑4629577 | tgggggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACg < 1:866830/68‑1 (MQ=255) gtggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTc > 1:187126/1‑68 (MQ=255) tggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCt < 1:1225661/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:3244148/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:1954090/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACa < 1:1226943/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAAAGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:3735084/68‑1 (MQ=255) ttCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGt < 1:443122/68‑1 (MQ=255) ccggcgtatccggcgtaGATTCGAAATGTTTAAGAATACGGCGGATCGTCATGTCTACAGTACGGTCg > 1:1556597/1‑68 (MQ=255) ggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTg > 1:3880257/1‑68 (MQ=255) cgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCg > 1:1456707/1‑68 (MQ=255) cgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCg > 1:3943621/1‑68 (MQ=255) tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:1246029/68‑1 (MQ=255) tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:104825/68‑1 (MQ=255) tagccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:4092760/65‑1 (MQ=255) ccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTc > 1:500249/1‑68 (MQ=255) cggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCa > 1:1474755/1‑68 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:4552275/68‑1 (MQ=255) gtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTc > 1:511082/1‑68 (MQ=255) cGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCg < 1:3042193/68‑1 (MQ=255) aaaTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGt < 1:2359319/68‑1 (MQ=255) aaaTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGt < 1:1948460/68‑1 (MQ=255) aaaTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGt > 1:4463544/1‑68 (MQ=255) tGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAt < 1:3517991/68‑1 (MQ=255) ttACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGATTCAGCTCACGGCCGGTCAtttt < 1:1348896/68‑1 (MQ=255) cGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCGGCTGACGGCCGGTCATTttctt > 1:946183/1‑68 (MQ=255) gAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTttcttc > 1:4707365/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:99981/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca < 1:3252797/68‑1 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca < 1:2570882/68‑1 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:1947808/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:1675816/1‑68 (MQ=255) ggcggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt < 1:3610296/68‑1 (MQ=255) ggcggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt < 1:1926786/68‑1 (MQ=255) ggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCa < 1:1912943/68‑1 (MQ=255) ggATCGTCATGTCTACAGAACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCa > 1:4131253/1‑68 (MQ=255) aTCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCTGCAGTTCAGc < 1:4251290/68‑1 (MQ=255) | TGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGC > CP009273/4629451‑4629577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |