| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 829,599 | G→T | M358I (ATG→ATT) | dinG → | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 829,599 | 0 | G | T | 97.6% | 128.0 / ‑3.6 | 42 | M358I (ATG→ATT) | dinG | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (11/30); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
GATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATT > CP009273/829536‑829662 | gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc > 1:4132442/1‑68 (MQ=255) ggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTgg < 1:3544185/68‑1 (MQ=255) gCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc < 1:223213/68‑1 (MQ=255) gCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc < 1:2774658/68‑1 (MQ=255) gCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc < 1:1680578/68‑1 (MQ=255) gAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCt < 1:2857245/68‑1 (MQ=255) aCTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTgg < 1:901246/68‑1 (MQ=255) cTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGc > 1:4134237/1‑68 (MQ=255) tGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCg > 1:1125796/1‑68 (MQ=255) cAGATTAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:1785121/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGTCAAAACTCACCGAGATTCTGCGTGGTCTGGCGGAg < 1:1936102/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCTCCGAGATTCTGCGTGGCCTGGCGGAg < 1:186800/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:2060420/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:2519884/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:451672/68‑1 (MQ=255) gAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTAtt > 1:3956254/1‑68 (MQ=255) tGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCtt > 1:2157999/1‑68 (MQ=255) ggAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACg > 1:3885021/1‑68 (MQ=255) ggAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACg > 1:3715598/1‑68 (MQ=255) agaTCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAt < 1:1656178/68‑1 (MQ=255) agaTCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAt < 1:880030/68‑1 (MQ=255) tCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTa > 1:4016177/1‑68 (MQ=255) ccAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTg > 1:4360634/1‑68 (MQ=255) ccAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTACTTAACGATTTAAGTg > 1:758756/1‑68 (MQ=255) ccAGCGGCGGGCAAAACTCAGCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTg < 1:2530866/68‑1 (MQ=255) cAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGc < 1:1635040/68‑1 (MQ=255) aaaaCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCa < 1:2816753/68‑1 (MQ=255) aaCTCACCGAGAATCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGc < 1:3258879/68‑1 (MQ=255) aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc < 1:2370394/68‑1 (MQ=255) aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc < 1:4047070/68‑1 (MQ=255) aCTCACCGAGATTCAGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc < 1:4529087/68‑1 (MQ=255) cTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCa > 1:3345239/1‑68 (MQ=255) cTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCa > 1:3962033/1‑68 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTTAGTGAGAAAACCGGCAGCCATGAc < 1:1209247/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTATGTGAGAAAACCGGCAGCCATGAc < 1:83831/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc < 1:231403/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc < 1:2041783/68‑1 (MQ=255) cGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACa < 1:3691899/68‑1 (MQ=255) cGAGATTCTGAGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACa < 1:2992535/68‑1 (MQ=255) gagaTTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACAt < 1:2239330/68‑1 (MQ=255) tgaTTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACAtt < 1:3177499/67‑1 (MQ=255) agaTTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACAtt < 1:306300/68‑1 (MQ=255) agaTTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACAtt < 1:146676/68‑1 (MQ=255) | GATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACATT > CP009273/829536‑829662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |