| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 2,681,289 | C→A | D292Y (GAT→TAT) | glrR ← | response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,681,289 | 0 | C | A | 97.7% | 140.1 / ‑4.3 | 45 | D292Y (GAT→TAT) | glrR | response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (26/17); total (27/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
GTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGC > CP009273/2681224‑2681353 | gTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATAtt > 1:2826178/1‑68 (MQ=255)gTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATAtt > 1:2747909/1‑68 (MQ=255) ttctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTcgc > 1:964049/1‑68 (MQ=255) tctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTcgcg > 1:2656535/1‑68 (MQ=255) ctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCgg > 1:1942984/1‑68 (MQ=255) gtgCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACACCGTTGAGGCGGTAATAGAGATATTCGCGGaa > 1:2667611/1‑68 (MQ=255) gcgcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAAtt > 1:1761104/1‑68 (MQ=255) gcgcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGGTGAGGCGGTAATAGAGATGTTAGCGGACtt > 1:3428803/1‑68 (MQ=255) gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc > 1:4084341/1‑68 (MQ=255) gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc > 1:3150391/1‑68 (MQ=255) gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTcc < 1:1854350/68‑1 (MQ=255) gTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGcc < 1:1211666/68‑1 (MQ=255) tGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCa > 1:3374160/1‑68 (MQ=255) ccGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATc > 1:2296788/1‑68 (MQ=255) ggAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGc < 1:4746575/68‑1 (MQ=255) ttttCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCtttt > 1:3110015/1‑68 (MQ=255) tttCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTg < 1:3776400/68‑1 (MQ=255) tttCAGCCTGACAACGGTGAGGCGGTAATAGAGATAATCGCGGAATTCCCCGCGCGCCATCGCTTTTg < 1:3379115/68‑1 (MQ=255) tCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGc > 1:2132840/1‑68 (MQ=255) gCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGAt < 1:2923853/68‑1 (MQ=255) cTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATc < 1:3236669/68‑1 (MQ=255) tGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCa < 1:3486386/68‑1 (MQ=255) gACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCAc < 1:3332825/68‑1 (MQ=255) aaCGTTGAGGCGTTAATAGAGATATTCTAGGAATTCCCCGCGCGCCATCGATTTTGGCAGATCACGGt > 1:769036/1‑68 (MQ=255) aaCGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGt > 1:1871187/1‑68 (MQ=255) cGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGa < 1:4131186/68‑1 (MQ=255) tGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTg > 1:4135716/1‑68 (MQ=255) gAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTgg < 1:1575100/68‑1 (MQ=255) gAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTgg < 1:1653527/68‑1 (MQ=255) ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGCGTGGCa > 1:1367959/1‑68 (MQ=255) ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCa < 1:3280556/68‑1 (MQ=255) cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:11495/68‑1 (MQ=255) cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:3635854/68‑1 (MQ=255) cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:1586329/68‑1 (MQ=255) aggTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:4756682/67‑1 (MQ=255) gagaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATcc > 1:1706367/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATcgg > 1:1510523/1‑66 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:411189/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:3488156/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:443116/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:451348/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:1850517/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:576690/1‑68 (MQ=255) gaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGc < 1:4741800/68‑1 (MQ=255) gaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGc > 1:57206/1‑68 (MQ=255) | GTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGC > CP009273/2681224‑2681353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |