Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,901,251 C→A G17V (GGT→GTT)  eno ← enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,901,2510CA97.7% 141.4 / ‑4.3 43G17V (GGT→GTT) enoenolase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (18/24);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTC  >  CP009273/2901186‑2901317
                                                                 |                                                                  
aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                                  <  1:2826110/68‑1 (MQ=255)
aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                                  <  1:3519152/68‑1 (MQ=255)
 cGGAGCTGCTGCCATACCGACTAAACCACCCTACAGATGTACTTCGGCTTCAACAGTCGGGTTAacac                                                                 <  1:3477525/68‑1 (MQ=255)
   gAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACgg                                                               >  1:351144/1‑68 (MQ=255)
    agcagcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACggg                                                              <  1:972324/68‑1 (MQ=255)
    agcagcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACggg                                                              <  1:535555/68‑1 (MQ=255)
               tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                   <  1:1614810/68‑1 (MQ=255)
               tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                   <  1:3614713/68‑1 (MQ=255)
                aCCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAttt                                                  >  1:2886409/1‑68 (MQ=255)
                      gaAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGAc                                            <  1:3115654/68‑1 (MQ=255)
                          ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACCACCgat                                        >  1:196481/1‑68 (MQ=255)
                              ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGAtt                                    >  1:1891453/1‑68 (MQ=255)
                                      tGTACTTCTGCTTCAACAGGCGGGTTAACACGGGAGTCTATGATTTCACGACCGATGATTTTTACGAt                            <  1:3546707/68‑1 (MQ=255)
                                      tGTACTTCGGCTTCAACCGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            <  1:4143462/68‑1 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            >  1:2742498/1‑68 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            >  1:2770158/1‑68 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            <  1:4736818/68‑1 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            >  1:701601/1‑68 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            <  1:4671210/68‑1 (MQ=255)
                                      tGTACTTCGGCTTCAACAGTCGGGTAAACACTGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                            <  1:2669623/68‑1 (MQ=255)
                                        tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                          >  1:2279573/1‑68 (MQ=255)
                                        tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                          >  1:3971274/1‑68 (MQ=255)
                                           ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                       >  1:2058504/1‑68 (MQ=255)
                                           ttCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTgg                       >  1:648242/1‑68 (MQ=255)
                                            tCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGa                      <  1:246813/68‑1 (MQ=255)
                                             cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                     <  1:1444875/68‑1 (MQ=255)
                                             cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                     <  1:4707720/68‑1 (MQ=255)
                                             cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                     <  1:1834277/68‑1 (MQ=255)
                                              ggCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACa                    <  1:653744/68‑1 (MQ=255)
                                                cTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACAtt                  >  1:1463126/1‑68 (MQ=255)
                                                   cAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAgg               <  1:4445418/68‑1 (MQ=255)
                                                    aaCAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGt              <  1:4445217/68‑1 (MQ=255)
                                                    aaCAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGt              <  1:1025642/68‑1 (MQ=255)
                                                    aaCAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGt              <  1:715110/68‑1 (MQ=255)
                                                     aCAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGtt             >  1:240822/1‑68 (MQ=255)
                                                       aGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGtttt           >  1:202732/1‑68 (MQ=255)
                                                         tCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTcc         <  1:3565736/68‑1 (MQ=255)
                                                         tCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTcc         <  1:3394910/68‑1 (MQ=255)
                                                          cGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCt        >  1:4184368/1‑68 (MQ=255)
                                                          cGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCt        >  1:983671/1‑68 (MQ=255)
                                                          cGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCt        >  1:4252108/1‑68 (MQ=255)
                                                            ggTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCa      >  1:980379/1‑68 (MQ=255)
                                                              ttAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAg    <  1:2251798/68‑1 (MQ=255)
                                                                aCCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTc  <  1:534654/68‑1 (MQ=255)
                                                                 |                                                                  
ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTC  >  CP009273/2901186‑2901317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑