| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,208,093 | T→C | F563S (TTC→TCC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,208,093 | 0 | T | C | 98.2% | 180.3 / NA | 54 | F563S (TTC→TCC) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/30); minor base G (0/1); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTT > CP009273/3208028‑3208151 | tgcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCACAAGTTCTGCGTATGCGTTCCg < 1:824348/68‑1 (MQ=255) gcgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCgg < 1:2961336/68‑1 (MQ=255) cgGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGt < 1:3810836/68‑1 (MQ=255) aCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGa < 1:3098139/68‑1 (MQ=255) aCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGa < 1:2996067/68‑1 (MQ=255) aCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGa < 1:2868888/68‑1 (MQ=255) aCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGa < 1:2001990/68‑1 (MQ=255) aCGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGa < 1:1746134/68‑1 (MQ=255) gCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGata < 1:2918516/68‑1 (MQ=255) gCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGata < 1:182849/68‑1 (MQ=255) gCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGATCTGCGTATGCGTTCCGGTATCGata < 1:2617627/68‑1 (MQ=255) acgacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATg > 1:53660/1‑68 (MQ=255) acgacgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATg > 1:2736203/1‑68 (MQ=255) acgTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAac > 1:3368829/1‑68 (MQ=255) tGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACAcc > 1:3850375/1‑68 (MQ=255) tGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACAcc < 1:2228762/68‑1 (MQ=255) ctggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTac > 1:429487/1‑68 (MQ=255) ggcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTacac < 1:775215/68‑1 (MQ=255) gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACg < 1:4229383/68‑1 (MQ=255) gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACg < 1:3590073/68‑1 (MQ=255) gcCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGGTCCGGTATCGATATGAACACCGACTACACg < 1:3032927/68‑1 (MQ=255) cTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCt < 1:1059272/68‑1 (MQ=255) aCCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGga < 1:2312428/68‑1 (MQ=255) ccGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGATGgaa > 1:2971431/1‑68 (MQ=255) ccGCGCGTGAAGCAAAAGTTCTGCGTATGCGGTCCGGTATCGATATGAACACCGACTACACGCGGgaa > 1:2678618/1‑68 (MQ=255) cgcgcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaag > 1:3167784/1‑68 (MQ=255) cgcgcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaag > 1:3930703/1‑68 (MQ=255) gcgcgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGgaaga < 1:3076959/68‑1 (MQ=255) cgTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGt > 1:4065627/1‑68 (MQ=255) gagAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCTATATGAACACCGACTACACGCTGGAAGAAGTg < 1:619414/66‑1 (MQ=255) gTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCTATATGAACACCGACTACACGCTGGAAGAAGTg < 1:2014783/68‑1 (MQ=255) gTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg < 1:1478878/68‑1 (MQ=255) gTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg < 1:2268706/68‑1 (MQ=255) gTGAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTg < 1:391155/68‑1 (MQ=255) gAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTggg < 1:2906502/68‑1 (MQ=255) gAAGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTcgg < 1:1621991/68‑1 (MQ=255) aGCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTa > 1:491269/1‑68 (MQ=255) gCAAAAGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTaa > 1:507336/1‑68 (MQ=255) aaaGTTCTTCGTATGCGTTGCGGTATCGATATGATCAGCGACTAGACGCTGGAAGAAGTGTGTAAACa < 1:2799622/68‑1 (MQ=255) aaaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACa < 1:2714311/68‑1 (MQ=255) aaGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAg < 1:13958/68‑1 (MQ=255) aGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt < 1:3786335/68‑1 (MQ=255) aGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt > 1:3557505/1‑68 (MQ=255) aGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt > 1:3329576/1‑68 (MQ=255) aGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt < 1:2654892/68‑1 (MQ=255) aGTTCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGt > 1:1561188/1‑68 (MQ=255) ttCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTACACAGTTc > 1:1933657/1‑68 (MQ=255) ttCTGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTc > 1:1939557/1‑68 (MQ=255) tGCGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGAc > 1:1259595/1‑68 (MQ=255) cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGt > 1:1591307/1‑68 (MQ=255) cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGt > 1:2155170/1‑68 (MQ=255) cGTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGt > 1:1810477/1‑68 (MQ=255) gTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGtt > 1:1340606/1‑68 (MQ=255) gTATGCGTTCCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGtt > 1:3591887/1‑68 (MQ=255) | TGCGGCAACGCACGACGTGCTGGCTGGCCTGACCGCGCGTGAAGCAAAAGTTCTGCGTATGCGTTTCGGTATCGATATGAACACCGACTACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTT > CP009273/3208028‑3208151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |