| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,466,500 | G→A | T125I (ACC→ATC) | fusA ← | protein chain elongation factor EF‑G, GTP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,466,500 | 0 | G | A | 98.2% | 192.1 / NA | 57 | T125I (ACC→ATC) | fusA | protein chain elongation factor EF‑G, GTP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/28); minor base T (1/0); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACG > CP009273/3466438‑3466564 | ggTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCa > 1:3296344/1‑68 (MQ=255)ggTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCa > 1:2007591/1‑68 (MQ=255) aTTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGCCGCCATACGATTTCAGACTg > 1:1323251/1‑68 (MQ=255) tttGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCg < 1:4457808/68‑1 (MQ=255) tttCTTAACGCACGCAATGCTCGGAACTTTATAATTGTTTCCCTGACGCCATACGATTTCAGACTGCg < 1:1373964/68‑1 (MQ=255) tttCTTAACGAACGCAATGCGCGGAACTTTATTTTTGTTTTCCTGACGCCATACGATTTCAGACTGCg < 1:2901680/68‑1 (MQ=255) tGTTATCGAACGCAAAGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGc < 1:185100/68‑1 (MQ=255) tGTTAACGAACGCAAAGCGCGCAACTTTATATTTTATTCCCTGACGCCATACGATTTCAGACTGCGGc < 1:4946586/68‑1 (MQ=255) tGTAAACGACCGCAATGCGCGGAACTTTATATTTGTTTGCCAGACGCCATACGATTTCAGACTGCGGc < 1:1812034/68‑1 (MQ=255) gTTAACGAACGCAATGCGCGGATCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCt > 1:1627694/1‑68 (MQ=255) gTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCt > 1:3471007/1‑68 (MQ=255) gTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCt > 1:680160/1‑68 (MQ=255) cgaacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAaca > 1:5475472/1‑68 (MQ=255) acgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAAcacca < 1:1209011/68‑1 (MQ=255) cAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACAccacca < 1:46747/68‑1 (MQ=255) aTGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAAc < 1:708299/68‑1 (MQ=255) gAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAg < 1:351072/68‑1 (MQ=255) gAACTTTATATTAGTTTGCCTGACGCCATACGATATCAGACTGCGGCTGAACACCACCAACTGCGCAg < 1:2445681/68‑1 (MQ=255) caaCTTTATATTTGTTTTGATGACGCCATACGATTTCAGACTGCGGCTGAACACCTCCAACTGCGCAg < 1:2708893/67‑1 (MQ=255) aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGt < 1:1639726/68‑1 (MQ=255) aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGt < 1:932426/68‑1 (MQ=255) aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGt < 1:4175168/68‑1 (MQ=255) aaCTTTATATTTGTTTGCCTGACG‑CATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTa < 1:2749266/68‑1 (MQ=255) tttATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTaaa < 1:957525/68‑1 (MQ=255) tttATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTaaa < 1:3333447/68‑1 (MQ=255) tttATATTTCATTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTaaa < 1:2270649/68‑1 (MQ=255) tttATATATGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTaaa < 1:6104512/68‑1 (MQ=255) atatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCACCTGCGCAGTAAACCa > 1:378703/1‑68 (MQ=255) atatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa < 1:867195/68‑1 (MQ=255) atatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa > 1:5914305/1‑68 (MQ=255) atTTGTTTGCCTGACGCCATTCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt > 1:4952196/1‑68 (MQ=255) atTTGTTTGCCTGACGCCATACGATTTCAGCCTGCGGCTGAACACCCCCAACTGCGCAGTAATCCAtt > 1:5628828/1‑68 (MQ=255) atTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt > 1:3499854/1‑68 (MQ=255) atTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt < 1:3934756/68‑1 (MQ=255) atTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt > 1:3105699/1‑68 (MQ=255) tttgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTa > 1:356299/1‑68 (MQ=255) tttgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTa > 1:1567828/1‑68 (MQ=255) tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGCACACCACCAACTGCGCAGTAAACCATTAcc > 1:583806/1‑68 (MQ=255) tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc > 1:130365/1‑68 (MQ=255) tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc > 1:90565/1‑68 (MQ=255) tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTCCACACCACCAACTGCGCAGTAATCCATTAcc > 1:4245084/1‑68 (MQ=255) tttgCCTGTCGCCATACGTTTTCAGACTGCGGCTGAACTCCCCCAACTGCGCAGTAAACCATTACCGc > 1:4205004/1‑68 (MQ=255) tttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTTCCGc > 1:4419313/1‑68 (MQ=255) tttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTGCCGc > 1:2606040/1‑68 (MQ=255) tttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGc > 1:6213403/1‑68 (MQ=255) tttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGc > 1:6219847/1‑68 (MQ=255) ccTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCa > 1:5981709/1‑68 (MQ=255) cTGACGCCATACGATTTCAGACTGCGGCTGAACACCCCCAACTGCGCAGTAAACCATTACCGCACCAt > 1:5578946/1‑68 (MQ=255) cTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCAt < 1:4361854/68‑1 (MQ=255) cTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCAt < 1:710001/68‑1 (MQ=255) gACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCg > 1:113773/1‑68 (MQ=255) gCCATACGATTTCAGACTGCGGCTGAGCACCACCAACTGCGCAGTAAACCATTACCGCACCATCgaga > 1:4938778/1‑68 (MQ=255) gCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCgaga > 1:3768723/1‑68 (MQ=255) aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg < 1:3900243/68‑1 (MQ=255) aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg < 1:2268774/68‑1 (MQ=255) aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg < 1:4404634/68‑1 (MQ=255) aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg < 1:2707729/68‑1 (MQ=255) | GGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACG > CP009273/3466438‑3466564 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |