| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 3,729,347 | G→A | E337K (GAG→AAG) | xylR → | xylose divergent operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,729,347 | 0 | G | A | 95.5% | 280.4 / 1.9 | 88 | E337K (GAG→AAG) | xylR | xylose divergent operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (2/2); new base A (39/45); total (41/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
TCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCA > CP009273/3729284‑3729411 | tCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGaa < 1:889102/68‑1 (MQ=255)tCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGaa < 1:4346001/68‑1 (MQ=255)tCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGaa > 1:3560970/1‑68 (MQ=255)tCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGaa < 1:720093/68‑1 (MQ=255)tCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGaa < 1:5207182/68‑1 (MQ=255) ctttCTTGAGAAGCGTTTTAAAGAAGAGGTGGTTGAAACCATCCATGCCATGATTCATGCCATGAAGc < 1:815306/65‑1 (MQ=255) cTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGga > 1:339689/1‑68 (MQ=255) cTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGga < 1:3452328/68‑1 (MQ=255) cTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGga < 1:5677947/68‑1 (MQ=255) agaAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGaaa > 1:3486229/1‑68 (MQ=255) gaAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAg > 1:4509918/1‑68 (MQ=255) gaAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAg < 1:2993298/68‑1 (MQ=255) aaGAGTTTTTAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgc < 1:299180/68‑1 (MQ=255) aGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgcg > 1:454175/1‑68 (MQ=255) aGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgcg > 1:1666766/1‑68 (MQ=255) aGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgcg > 1:3966653/1‑68 (MQ=255) aGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgcg > 1:556043/1‑68 (MQ=255) aGCGTTTTAAAGAAGAGGTGGGGGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAgcg < 1:167842/68‑1 (MQ=255) cGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcgcg > 1:3781724/1‑68 (MQ=255) gTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGTAAgcgcgc > 1:830318/1‑68 (MQ=255) ttttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCa < 1:2932592/68‑1 (MQ=255) tttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAg < 1:2738360/68‑1 (MQ=255) ttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGt < 1:4842769/68‑1 (MQ=255) ttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGt < 1:140232/68‑1 (MQ=255) ttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGt < 1:1317788/68‑1 (MQ=255) ttAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGt < 1:1089425/68‑1 (MQ=255) ttAAAGAAGAGGTGGGGGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGt < 1:4100914/68‑1 (MQ=255) tAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTc < 1:249809/68‑1 (MQ=255) tAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTc < 1:5382230/68‑1 (MQ=255) aaaGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTct < 1:4249385/68‑1 (MQ=255) aaaGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTct < 1:3140538/68‑1 (MQ=255) agaagaGGTGGGTGATACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTctgc > 1:4906816/1‑68 (MQ=255) agaagaGGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTctgc < 1:289160/68‑1 (MQ=255) agagGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGATGCTGGAGAAAGCGCGCAGTCTGCTGa > 1:1954433/1‑68 (MQ=255) agagGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGa < 1:2429937/68‑1 (MQ=255) agagGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGa < 1:3846521/68‑1 (MQ=255) agagGTGGGGGAAACCATCCATGCCATGATTCATGCCAAGAAGCTTGAGAAAGCGCGCAGTCTGCTGa < 1:4700298/68‑1 (MQ=255) agagGTGGGGGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGa < 1:4700300/68‑1 (MQ=255) agGTGGGTGAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAtt > 1:2321635/1‑68 (MQ=255) ggtgggtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGCttt < 1:1289279/68‑1 (MQ=255) ggtgggtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAttt > 1:4565245/1‑68 (MQ=255) ggtgggagAAACCATCCATGCCATGAATCATGCCGAGAAGCTGGAGAAACCGCGCAGTCTGCTGAttt < 1:1463579/68‑1 (MQ=255) tgggtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCa > 1:1553746/1‑68 (MQ=255) gtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacc > 1:3522872/1‑68 (MQ=255) gtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacc > 1:5527166/1‑68 (MQ=255) gtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacc < 1:520060/68‑1 (MQ=255) gtgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacc > 1:5601627/1‑68 (MQ=255) gggAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAacc < 1:5851824/66‑1 (MQ=255) tgAAACCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGA‑TTCAaccac < 1:53149/68‑1 (MQ=255) aaCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCt < 1:1006709/68‑1 (MQ=255) aCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCTGTCTGCTGATTTCAACCACCtt > 1:2794263/1‑68 (MQ=255) aCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCtt < 1:1555104/68‑1 (MQ=255) aCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCtt > 1:1422285/1‑68 (MQ=255) aCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCtt > 1:4924305/1‑68 (MQ=255) aCCATCCATGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCtt < 1:4881092/68‑1 (MQ=255) tccatGCCATGATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCg > 1:1330140/1‑68 (MQ=255) ccatgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGa > 1:4123474/1‑68 (MQ=255) ccaagccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGa < 1:1580310/68‑1 (MQ=255) catgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGAt < 1:2550402/68‑1 (MQ=255) catgcaatgATTCATGCCAAGAAGCTTGAGAAAGCGCGCAGTCTGCTGTTATCAACCACCTTGTCGAt < 1:3204923/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:45507/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:3994989/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:4901152/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:2761063/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:2129245/68‑1 (MQ=255) tgccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCa < 1:166570/68‑1 (MQ=255) tgccatgAATCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATATCAACCACCTTGTCGATCa < 1:585374/68‑1 (MQ=255) ccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt > 1:5180939/1‑68 (MQ=255) ccatgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt > 1:1091798/1‑68 (MQ=255) catgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATg > 1:4726737/1‑68 (MQ=255) catgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATg > 1:4735681/1‑68 (MQ=255) catgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATg > 1:4798336/1‑68 (MQ=255) catgATTCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATg > 1:5381058/1‑68 (MQ=255) gaatCATGCCAAGAAGCTGGAGAAAGCGCGCAGGCTGCTGATTTCAACCACCTTGTCGATCAATgaga < 1:3204010/65‑1 (MQ=255) ttCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGata > 1:980260/1‑68 (MQ=255) ttCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGata > 1:3749317/1‑68 (MQ=255) ttCATGCCAAGAAGCTGGAGAAAGCGCGCAGGCTGCTGATTTCAACCACCTTGTCGATCAATGAGata < 1:1105659/68‑1 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:5121910/1‑68 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:3994049/1‑68 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:3070774/1‑68 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:2485550/1‑68 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:186291/1‑68 (MQ=255) tCATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGatat > 1:1160491/1‑68 (MQ=255) cATGCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATc < 1:6079451/68‑1 (MQ=255) gCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCa > 1:3147150/1‑68 (MQ=255) gCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCa > 1:4050074/1‑68 (MQ=255) gCCAAGAAGCTGGAGAAAGCGCGCAGTCTGCTCATTTCAACCACCTTGTCGATCAATGAGATATCGCa > 1:4322949/1‑68 (MQ=255) gCCAAGAAGCGGGAGAACGCGCGCAGTCTGCTGATTTCAACCCCCTTGTCGATCAATGAGATTTCGCa > 1:1056625/1‑68 (MQ=255) | TCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCA > CP009273/3729284‑3729411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |