| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 4,173,770 | T→G | D866E (GAT→GAG) | rpoB → | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,173,770 | 0 | T | G | 98.6% | 238.9 / ‑4.8 | 71 | D866E (GAT→GAG) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (22/48); total (23/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGT > CP009273/4173707‑4173832 | gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGTGTGAAGCTGCGCTCTCCATACTGGAGGAAt < 1:3323028/68‑1 (MQ=255)gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt < 1:94591/68‑1 (MQ=255)gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt < 1:3093488/68‑1 (MQ=255)gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt < 1:4786755/68‑1 (MQ=255)gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt < 1:2404707/68‑1 (MQ=255)gggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAAt < 1:1931244/68‑1 (MQ=255) ggTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATc > 1:2686446/1‑68 (MQ=255) tCCGGAAGAGATCACCGCTGACATCACGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCg > 1:3271963/1‑68 (MQ=255) ccGGATGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCgg > 1:759941/1‑68 (MQ=255) gAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTAt < 1:2161968/68‑1 (MQ=255) gAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTAt < 1:4082576/68‑1 (MQ=255) aaGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATc < 1:4559298/68‑1 (MQ=255) tgagaTCACCGCTGACATCCCGAACGTGTGGGACGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:1889708/67‑1 (MQ=255) agagagCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:4879867/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCTAACTGGAGGAATCCGGTATCg < 1:3395348/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:5026691/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:3629094/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:965464/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg > 1:3230234/1‑68 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:144319/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:4248527/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:5341786/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:3676159/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:4881260/68‑1 (MQ=255) agagATCACCGCTGACATCCCGAACGTGGGTGAACCTGCGCTCTCCAAACTGGAGGAATCCGGTAACg > 1:4423875/1‑68 (MQ=255) agagACCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCg < 1:2870542/68‑1 (MQ=255) gagaTCACCGCTGACATCCCGAACGTGGGGGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGt < 1:1821172/68‑1 (MQ=255) gaTCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGttt < 1:395714/68‑1 (MQ=255) gaTCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGttt < 1:4798794/68‑1 (MQ=255) tCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTAc > 1:2712590/1‑68 (MQ=255) tCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTAc < 1:1114053/68‑1 (MQ=255) aCCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACAt > 1:497687/1‑68 (MQ=255) ccGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACAtt > 1:995782/1‑68 (MQ=255) cTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGt < 1:6004216/68‑1 (MQ=255) cTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGt < 1:314540/68‑1 (MQ=255) cTGACATCCCGAACGTGGGTGAAGCTGCGCGCTCCAAACTGGAGGAATCCGGAATCGTTTACATTGGt < 1:5170596/68‑1 (MQ=255) cATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGTTATCGTTTACAATGGTGCgg > 1:2965980/1‑68 (MQ=255) aTCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGa < 1:3731601/68‑1 (MQ=255) cccGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAg > 1:347482/1‑68 (MQ=255) cccGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAg > 1:1334136/1‑68 (MQ=255) cccGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAg < 1:2510171/68‑1 (MQ=255) cGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCg > 1:3434169/1‑68 (MQ=255) cGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCg > 1:4139423/1‑68 (MQ=255) cGTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTAACATTGGAGCGGCAGTCACCg > 1:5546637/1‑68 (MQ=255) gTGGGTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCgg < 1:102100/68‑1 (MQ=255) gTGAAGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGc < 1:5950480/68‑1 (MQ=255) aaGCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGAc < 1:4018937/68‑1 (MQ=255) gCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAt > 1:820048/1‑68 (MQ=255) gCTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAt > 1:1980102/1‑68 (MQ=255) cTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAtt > 1:2880835/1‑68 (MQ=255) cTGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACAtt > 1:5199083/1‑68 (MQ=255) tGCGCTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACTTTc > 1:4300894/1‑68 (MQ=255) gcgcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCt < 1:5116580/68‑1 (MQ=255) gcTCTCCATACTGGAGGAATCCGGGATCGTTTACATTGGGGCGGAACTGCCCGGTGGCGCCATTCtgg > 1:3861923/1‑68 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtgg > 1:1512342/1‑68 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtgg < 1:3171602/68‑1 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtgg > 1:5335292/1‑68 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtgg < 1:1532723/68‑1 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtgg < 1:4799727/68‑1 (MQ=255) gcTCTCCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGGGACCGGTGGCGACATTCtgg < 1:2932905/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:5745520/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:1312714/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:5946908/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:2009909/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:6157438/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttg < 1:1339954/68‑1 (MQ=255) ctcCAAACTGGAGGAATCCGGTATCGTTTACATTGGCGCGGAAGAGACCGGTGGCGACATTCtggttg < 1:6155997/68‑1 (MQ=255) ccAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttggt < 1:5612814/68‑1 (MQ=255) ccAAACTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCtggttggt < 1:5776533/68‑1 (MQ=255) aCTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTggtaagg < 1:1980452/68‑1 (MQ=255) cTGGAGGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTggtaaggt > 1:349201/1‑68 (MQ=255) | GGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGTATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGT > CP009273/4173707‑4173832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |