Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,275,144	C→T	intergenic (‑3/+197)	yjcH ← / ← acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,275,144	0	C	T	100.0%	273.8 / NA	83	intergenic (‑3/+197)	yjcH/acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/40);  total (43/40)

TTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCT  >  CP009273/4275081‑4275210                                                                |                                                                   tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGAt                                                                <  1:1291863/68‑1 (MQ=255) tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGAt                                                                <  1:5983566/68‑1 (MQ=255) tttCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGAt                                                                <  1:1382247/68‑1 (MQ=255)    cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                             <  1:4209153/68‑1 (MQ=255)    cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                             <  1:1914312/68‑1 (MQ=255)    cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                             <  1:6090318/68‑1 (MQ=255)       cTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCa                                                          >  1:1279632/1‑68 (MQ=255)         aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCaga                                                        <  1:3547490/68‑1 (MQ=255)          aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                       <  1:2308524/68‑1 (MQ=255)          aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                       <  1:2723222/68‑1 (MQ=255)          aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                       <  1:3003569/68‑1 (MQ=255)            tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                     <  1:6029982/68‑1 (MQ=255)            tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                     <  1:2811955/68‑1 (MQ=255)            tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                     <  1:5327358/68‑1 (MQ=255)              ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGTGGTaa                                                   <  1:1154789/68‑1 (MQ=255)              ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTaa                                                   <  1:6145300/68‑1 (MQ=255)              ccTGAAATGCGCATTGTCTTCTATCAGCTGATAAATAGTGCCATTCATCATAGATTCTCCATAGGTaa                                                   >  1:4148777/1‑68 (MQ=255)               cTGATATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAAt                                                  <  1:5966268/68‑1 (MQ=255)               cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAAt                                                  <  1:2536463/68‑1 (MQ=255)                tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTTATCATAGATTCTCCAGAGGTAATg                                                 <  1:3404077/68‑1 (MQ=255)                 gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGt                                                >  1:3619711/1‑68 (MQ=255)                  aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa                                               >  1:4635806/1‑68 (MQ=255)                  aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa                                               <  1:4271168/68‑1 (MQ=255)                  aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa                                               >  1:5063980/1‑68 (MQ=255)                  aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa                                               >  1:4994231/1‑68 (MQ=255)                   aaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAg                                              >  1:5476271/1‑68 (MQ=255)                    aTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAgg                                             >  1:1743351/1‑68 (MQ=255)                     tGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAggg                                            >  1:3828308/1‑68 (MQ=255)                     tGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAggg                                            <  1:1346144/68‑1 (MQ=255)                        gcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGAtt                                         >  1:542287/1‑68 (MQ=255)                         cATTGTCTTCTATCCGCTGATAAATACTGCCATTCATCATAGATTCTCCAGAGGTAATGAAGGGATTg                                        <  1:5519115/68‑1 (MQ=255)                         cATTGTCTACTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTg                                        <  1:4662074/68‑1 (MQ=255)                          aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                       <  1:2798194/68‑1 (MQ=255)                          aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                       <  1:2285623/68‑1 (MQ=255)                          aTTGTCTTCTATCCGCTGATAAATAGTGCCATTAATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                       <  1:6181358/68‑1 (MQ=255)                           ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtt                                      <  1:3334933/68‑1 (MQ=255)                           ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtt                                      <  1:2243118/68‑1 (MQ=255)                             gTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                    >  1:1449477/1‑68 (MQ=255)                                  ctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                               >  1:5492518/1‑68 (MQ=255)                                  ctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                               >  1:4114028/1‑68 (MQ=255)                                    aTCCGCTGATATATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTctg                             >  1:4000940/1‑66 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:345902/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:585872/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:5459375/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:6005489/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:4423500/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCACAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:4396447/1‑68 (MQ=255)                                    aTCCGCTGATAAATAGTGCCATTCATCATAGAATCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                             >  1:3183459/1‑68 (MQ=255)                                     tCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCg                            >  1:2575137/1‑68 (MQ=255)                                      ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                           >  1:3180191/1‑68 (MQ=255)                                      ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                           >  1:2740335/1‑68 (MQ=255)                                      ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                           >  1:4994331/1‑68 (MQ=255)                                      ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTACCGc                           >  1:1041092/1‑68 (MQ=255)                                        gCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc                         >  1:4281692/1‑68 (MQ=255)                                          tGATATATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                       <  1:3924260/68‑1 (MQ=255)                                          tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                       <  1:2492036/68‑1 (MQ=255)                                          tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                       <  1:677093/68‑1 (MQ=255)                                          tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                       <  1:114473/68‑1 (MQ=255)                                          tGATAAATAGTGCCAATCATCATAGATTCTCCAGTGGTTATGTTGTGATTGTTTTAATTCCCGCTccc                       <  1:1082645/68‑1 (MQ=255)                                             tAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                    <  1:4778042/68‑1 (MQ=255)                                              aaaTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTAt                   <  1:4666223/68‑1 (MQ=255)                                                aTAGTGCCATTCCTCATAGATTCTCCAGAGGTAATGTAGGGATGGTTTTAATTCCCGCTCCCTTATgg                 >  1:2095574/1‑68 (MQ=255)                                                aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                 >  1:1075842/1‑68 (MQ=255)                                                aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                 >  1:5150718/1‑68 (MQ=255)                                                aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                 >  1:4262650/1‑68 (MQ=255)                                                 tAGTGCCATTCATCATAGATTCTCCAGAGGTACTGTAGGGATTGTTGTAATTCCCGCTCCCTTATggg                <  1:4580312/68‑1 (MQ=255)                                                  aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga               >  1:929558/1‑68 (MQ=255)                                                  aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga               >  1:909046/1‑68 (MQ=255)                                                  aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga               >  1:5204988/1‑68 (MQ=255)                                                    tGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgaga             <  1:323325/68‑1 (MQ=255)                                                     gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa            >  1:5859294/1‑68 (MQ=255)                                                     gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa            >  1:235173/1‑68 (MQ=255)                                                     gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa            >  1:5052977/1‑68 (MQ=255)                                                     gCCATTCATCATACATTCTCCAGAGGAAAAGTAGGGATTGTTTTAATTGCCGCTCCCTTATGGGAGaa            >  1:532154/1‑68 (MQ=255)                                                      ccATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg           <  1:249018/68‑1 (MQ=255)                                                      ccATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg           <  1:1673449/68‑1 (MQ=255)                                                       cATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg          >  1:3479668/1‑68 (MQ=255)                                                        aTTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGt         >  1:5051839/1‑68 (MQ=255)                                                          tcatcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa       >  1:4510624/1‑68 (MQ=255)                                                          tcatcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa       >  1:2512446/1‑68 (MQ=255)                                                            atcaTAGATTCTCCAGAGGTAATGTAGGGTTTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc     >  1:1708629/1‑68 (MQ=255)                                                            atcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc     <  1:3716170/68‑1 (MQ=255)                                                            atcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc     <  1:1878783/68‑1 (MQ=255)                                                               acaGATTCTCCAGAGGTTATGTTGGCAGTGTTTTAAATCCCGCTCCCTTATGGGAGAATGTTAACGCt  >  1:5320560/1‑68 (MQ=255)                                                                |                                                                   TTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCT  >  CP009273/4275081‑4275210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑