| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 4,629,517 | C→T | V203M (GTG→ATG) | arcA ← | response regulator in two‑component regulatory system with ArcB or CpxA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,629,517 | 0 | C | T | 96.7% | 190.3 / ‑1.8 | 60 | V203M (GTG→ATG) | arcA | response regulator in two‑component regulatory system with ArcB or CpxA |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (30/28); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCA > CP009273/4629453‑4629575 | gtggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTc < 1:631259/68‑1 (MQ=255) tggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCt < 1:88244/68‑1 (MQ=255) tggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCt < 1:5343747/68‑1 (MQ=255) gggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCt < 1:5562064/67‑1 (MQ=255) ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTa > 1:6142980/1‑68 (MQ=255) ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTa > 1:4253990/1‑68 (MQ=255) ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTa > 1:2901796/1‑68 (MQ=255) ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTa > 1:5309516/1‑68 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:2319076/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:3820075/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:5229862/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:2160136/68‑1 (MQ=255) cGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACa < 1:534975/68‑1 (MQ=255) atgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGATTACGGCGGATCGTCATGTCTACAGt < 1:1726629/68‑1 (MQ=255) tgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTa > 1:4111029/1‑68 (MQ=255) ggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTg > 1:3488265/1‑68 (MQ=255) cgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCg > 1:4628814/1‑68 (MQ=255) gtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCgg < 1:50336/68‑1 (MQ=255) tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCTTCATGTCTACAGTACGGTCGTGCGGt < 1:473846/68‑1 (MQ=255) tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:3138340/68‑1 (MQ=255) tatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:4089050/68‑1 (MQ=255) tatccggcgtaGATTCGAAATGTATACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:5424826/68‑1 (MQ=255) aatccggcgtaGATTCGAAATGGTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGt < 1:5396872/67‑1 (MQ=255) atccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGtt > 1:724496/1‑68 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:4341857/68‑1 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:4067130/68‑1 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:4072212/68‑1 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:1194987/68‑1 (MQ=255) gcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:2338165/68‑1 (MQ=255) gagtaGATTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGc < 1:4146823/66‑1 (MQ=255) aTTCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACgg < 1:5521715/68‑1 (MQ=255) ttCGAAATGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGCTCGTGCGGTTTCAGCTCACGGc > 1:532033/1‑68 (MQ=255) aaaTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGt < 1:1286765/68‑1 (MQ=255) aaTGTTTACGAATACGGCGGCTCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTc > 1:838208/1‑68 (MQ=255) aTGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCa > 1:4677121/1‑68 (MQ=255) tGTTTACGAATACGGCGGATCGTGATGTCTACAGTACGGTCGTGCGGTTTCAGCTGACGGCCGGTCAt > 1:5841340/1‑68 (MQ=255) tGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAt > 1:5378278/1‑68 (MQ=255) tGTTTACGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCAt > 1:64123/1‑68 (MQ=255) aCGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTttct < 1:2542989/68‑1 (MQ=255) cGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTttctt > 1:2479187/1‑68 (MQ=255) cGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTttctt > 1:2328591/1‑68 (MQ=255) cGAATACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTttctt > 1:1741178/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:1608569/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:11503/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:2290905/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:1735743/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:2965751/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca < 1:45767/68‑1 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:1038315/1‑68 (MQ=255) aaTACGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:4044367/1‑68 (MQ=255) aaTACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTca > 1:5532787/1‑68 (MQ=255) aCGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagca > 1:5413056/1‑68 (MQ=255) aCGGCGGATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagca > 1:5430726/1‑68 (MQ=255) aCGGCGGATCGTCATGTCTACAGTACGGGCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagca > 1:1263813/1‑68 (MQ=255) cggcggATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagcag > 1:4762760/1‑68 (MQ=255) ggcggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTcagcagg < 1:4709640/68‑2 (MQ=255) ggcggATCGTCATGTCTACAGTACGGTCGTCCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGt < 1:1111255/68‑1 (MQ=255) cggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTc > 1:3004353/1‑68 (MQ=255) ggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCa > 1:5329323/1‑68 (MQ=255) ggATCGTCATGTCTACAGTACGGTCGTGCGGTTTCAGCGCACGGCCGGGCATTGTCTTCAGCAGTTCa > 1:691738/1‑68 (MQ=255) | GTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCA > CP009273/4629453‑4629575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |