Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	155,132	C→G	R161P (CGC→CCC)	pcnB ←	poly(A) polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	155,132	0	C	G	100.0%	157.0 / NA	49	R161P (CGC→CCC)	pcnB	poly(A) polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (32/17);  total (32/17)

CGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTT  >  CP009273/155067‑155190                                                                  |                                                           cgccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCgggg                                                          <  1:3635891/68‑1 (MQ=255)  gccAACGTAATCACGGTCTGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCggggc                                                         <  1:1071803/68‑1 (MQ=255)  gccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCggggc                                                         >  1:2654540/1‑68 (MQ=255)   ccAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAAACggggcg                                                        >  1:2835718/1‑68 (MQ=255)     aaCGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCt                                                      <  1:2267556/68‑1 (MQ=255)        gTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTggg                                                   >  1:1487729/1‑68 (MQ=255)          aaTCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCg                                                 <  1:6018615/68‑1 (MQ=255)             cACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                              >  1:559082/1‑68 (MQ=255)             cACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtct                                              <  1:3357600/68‑1 (MQ=255)              acggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtctt                                             <  1:5488711/68‑1 (MQ=255)                ggactgTAAACTCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                           <  1:743432/68‑1 (MQ=255)                ggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGACATCGGGGCGCTGGGCGtcttct                                           >  1:5957370/1‑68 (MQ=255)                ggacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGtcttct                                           <  1:3172946/68‑1 (MQ=255)                ggacggTAAAATCCGCTACGCTGTAATACAGGCTGGTGATAGTGACATCGGGGCGCTGGGCGtcttct                                           >  1:5257134/1‑68 (MQ=255)                 gacggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTcttctt                                          >  1:3938002/1‑68 (MQ=255)                  acggTAAAATCCGCTACGCTGTAATACATGCTGTTGATAGTCAAATCGGGGCGCTGGGCGTCttcttc                                         >  1:460002/1‑68 (MQ=255)                  acggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCttcttc                                         >  1:71228/1‑68 (MQ=255)                  acggTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCttcttc                                         >  1:2916450/1‑68 (MQ=255)                         aaTCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAg                                  >  1:2174390/1‑68 (MQ=255)                         aaTCCGCTACGCTGTAATACAGGCTGATGATAGTGAAATCGGGGCGCGGGGCGTCTTCTTCGATGGAg                                  <  1:3827824/68‑1 (MQ=255)                           tCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGcc                                <  1:6056054/68‑1 (MQ=255)                           tCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGcc                                <  1:4328155/68‑1 (MQ=255)                            ccGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCg                               >  1:4863987/1‑68 (MQ=255)                               cTACGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGaaa                            <  1:2465378/68‑1 (MQ=255)                                 aCGCTGTAATACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAt                          >  1:4527092/1‑68 (MQ=255)                                 aCGCTGTAATACAGGCTGTTGAGAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAAt                          <  1:327236/68‑1 (MQ=255)                                        aaTACAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTAGTcg                   >  1:4883076/1‑68 (MQ=255)                                           aCAGGCTGTTGATAGTGAAATCGGGGCGGTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                >  1:457189/1‑68 (MQ=255)                                           aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                >  1:2359235/1‑68 (MQ=255)                                           aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                >  1:5370160/1‑68 (MQ=255)                                           aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                >  1:4452851/1‑68 (MQ=255)                                           aCAGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCgcgc                >  1:5565705/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:6092908/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:5731505/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:1472415/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:3140951/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:1677590/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGAgcag              >  1:1130872/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTCTCGCgcag              >  1:1672234/1‑68 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTACGATGGAGCCGTAAATGTTGTCGCgcac              >  1:4387143/1‑67 (MQ=255)                                             aGGCTGTTGATAGTGAAATCGGGGCGCTGGGCGTATTCTTCGATGGAGCCGAAAATGTTGTCGCgcag              >  1:5025061/1‑68 (MQ=255)                                              ggCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcagc             <  1:4414330/68‑1 (MQ=255)                                               gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcagca            >  1:553395/1‑68 (MQ=255)                                               gCTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCgcagca            >  1:2468499/1‑68 (MQ=255)                                                cTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCaa           <  1:1322237/68‑1 (MQ=255)                                                cTGTTGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCaa           <  1:1313377/68‑1 (MQ=255)                                                  gTTGATTGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACa         <  1:5184698/68‑1 (MQ=255)                                                   ttGATAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACAt        >  1:511606/1‑68 (MQ=255)                                                     gATAGTGAAATCGGGGCGCTGGGCGTCTTCTTAGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGc      >  1:4194208/1‑68 (MQ=255)                                                       tAGTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCg    <  1:732521/68‑1 (MQ=255)                                                       tAGTGAAATCGGGGCGCTGGGCGGCTTCTGCGATCGAGCCGAAAATGTTGTCGCGCAGCAACATGCCg    <  1:3252681/68‑1 (MQ=255)                                                         gTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACCTGCCggt  >  1:4119364/1‑66 (MQ=255)                                                         gTGAAATCGGGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGtt  >  1:1205/1‑68 (MQ=255)                                                                  |                                                           CGCCAACGTAATCACGGACGGTAAAATCCGCTACGCTGTAATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTT  >  CP009273/155067‑155190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑