| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 829,599 | G→T | M358I (ATG→ATT) | dinG → | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 829,599 | 0 | G | T | 95.1% | 124.5 / ‑3.9 | 42 | M358I (ATG→ATT) | dinG | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (9/30); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACA > CP009273/829534‑829660 | gCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttct > 1:4193767/1‑68 (MQ=255) cGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctg > 1:5783896/1‑68 (MQ=255) gATGGGCGAACTGCCTGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc > 1:2538664/1‑68 (MQ=255) gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGTCAAAACTCACCGAGAttctgc > 1:5174984/1‑68 (MQ=255) gATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGAttctgc > 1:4805592/1‑68 (MQ=255) gATGGGCGAACTGCCAGATGAAGTGATGGAGATCTTCCAGCGGCTGACAAAACTCACCGAGAttctgc > 1:5876014/1‑68 (MQ=255) ggCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTgg < 1:144963/68‑1 (MQ=255) gCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc < 1:2255725/68‑1 (MQ=255) gCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGc < 1:4087886/68‑1 (MQ=255) cGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGcc > 1:1780765/1‑68 (MQ=255) aCTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCAGCGTGGCCTgg < 1:3615486/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:2270429/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:5714721/68‑1 (MQ=255) cAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAg < 1:2343232/68‑1 (MQ=255) gATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGtt > 1:2193988/1‑68 (MQ=255) gAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTAtt > 1:4174053/1‑68 (MQ=255) gAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTCCGTGGCCTGGCGGAGTTAtt < 1:1302288/68‑1 (MQ=255) aGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTc < 1:4544230/68‑1 (MQ=255) aGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTc < 1:5968017/68‑1 (MQ=255) tGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCtt > 1:3696597/1‑68 (MQ=255) ggAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACg < 1:1962589/68‑1 (MQ=255) ggAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACg < 1:5607990/68‑1 (MQ=255) aTCTGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGAttt > 1:271696/1‑68 (MQ=255) tGCCAGCGGCTGGCTAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAg > 1:6098103/1‑68 (MQ=255) tGCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAg < 1:3065256/68‑1 (MQ=255) gCCAGCGGCTGGCAATACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTAGATCTTAACGATTGAAGt > 1:2852158/1‑68 (MQ=255) gCCAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGt > 1:3169576/1‑68 (MQ=255) gCCAGCGGCTGGCAAAACTCACCGAGATACTGCCTGGCCTGGCGGAGTTATTTCTTAACGATTTAAga > 1:850899/1‑67 (MQ=39) ccAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTg > 1:672213/1‑68 (MQ=255) ccAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTg < 1:1875419/68‑1 (MQ=255) cAGCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTga < 1:5785109/68‑1 (MQ=255) gCGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTgaga < 1:3133632/68‑1 (MQ=255) cGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGaa > 1:5899873/1‑68 (MQ=255) cGGCTGGCAAAACTCACCGAGATTCTGCGTGGCCGGGCGTAGTTATTTCTAAACGATTTAAGTGAGaa < 1:5232483/68‑1 (MQ=255) gCTGGCAAAACTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGaaaa < 1:4901244/68‑1 (MQ=255) aaaCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAg < 1:1103691/68‑1 (MQ=255) aaCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGc < 1:5721763/68‑1 (MQ=255) aaCTCACCGAGATGCTGCGTGGCCTGGCGGAGTTAATTCTTCACGATTTAAGTGAGAAAACCGGCAGc < 1:5515760/68‑1 (MQ=255) aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc < 1:4436049/68‑1 (MQ=255) aCTCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGcc < 1:6098240/68‑1 (MQ=255) tCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCAt < 1:1879677/68‑1 (MQ=255) tCACCGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCAt < 1:4537668/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc < 1:3572789/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc < 1:4226906/68‑1 (MQ=255) ccGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGAc < 1:5200114/68‑1 (MQ=255) cGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACa < 1:4025567/68‑1 (MQ=255) cGAGATTCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCAATGACa < 1:842177/68‑1 (MQ=255) cGAGATTCTGCGGGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACa < 1:4989191/68‑1 (MQ=255) | GCGATGGGCGAACTGCCAGATGAAGTGCTGGAGATCTGCCAGCGGCTGGCAAAACTCACCGAGATGCTGCGTGGCCTGGCGGAGTTATTTCTTAACGATTTAAGTGAGAAAACCGGCAGCCATGACA > CP009273/829534‑829660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |