| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP009273 | 2,681,289 | C→A | D292Y (GAT→TAT) | glrR ← | response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,681,289 | 0 | C | A | 97.1% | 108.0 / ‑3.9 | 34 | D292Y (GAT→TAT) | glrR | response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (22/11); total (23/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGC > CP009273/2681224‑2681353 | gTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATAtt > 1:3075733/1‑68 (MQ=255) ttctGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTcgc > 1:2124014/1‑68 (MQ=255) gtgCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGaa < 1:1379031/68‑1 (MQ=255) gtgCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGaa > 1:1686393/1‑68 (MQ=255) gcgcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAAtt > 1:2094554/1‑68 (MQ=255) gcTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATAATCGCGGAATTcc > 1:4884435/1‑68 (MQ=255) ccGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATc > 1:5997659/1‑68 (MQ=255) tttCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTg < 1:5222580/68‑1 (MQ=255) tCAGGCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGc < 1:953549/68‑1 (MQ=255) ggCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGa > 1:4733748/1‑68 (MQ=255) gCTGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGAt < 1:5124059/68‑1 (MQ=255) tGACAACGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCa < 1:583569/68‑1 (MQ=255) aaCGTTGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGt < 1:2543517/68‑1 (MQ=255) gTTGAGGCGGTAATAGAGATATTCGCGGAATT‑CCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGt < 1:2044314/68‑1 (MQ=255) ttGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGt > 1:4295254/1‑68 (MQ=255) ttGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTCGCAGATCACGGTGAGt > 1:2837916/1‑68 (MQ=255) tGAGGCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTg > 1:812644/1‑68 (MQ=255) ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCa < 1:5178410/68‑1 (MQ=255) ggCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGACTgtct > 1:771439/1‑65 (MQ=255) gCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAg > 1:3922796/1‑68 (MQ=255) gCGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAg > 1:4628597/1‑68 (MQ=255) cGGTAATAGAGATATTCGCGGAATTCCCCGCGCGCGATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:5517917/68‑1 (MQ=255) cGGTAATAGAGATATTCGCGGAAATCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:5223023/68‑1 (MQ=255) aggTAATAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGa < 1:1113383/67‑1 (MQ=255) tAGAGATATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAaataat > 1:830072/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCTGAAATAATCCg > 1:5097169/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:1808392/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:5405506/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:1453049/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:654702/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:2003000/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:4237936/1‑68 (MQ=255) agaTATTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCg > 1:3364261/1‑68 (MQ=255) gaTCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGc > 1:2209134/1‑68 (MQ=255) | GTCTTCTGTGCGCTCCGCCAGTGCCGGAATTTTCAGGCTGACAACGTTGAGGCGGTAATAGAGATCTTCGCGGAATTCCCCGCGCGCCATCGCTTTTGGCAGATCACGGTGAGTGGCAGAAATAATCCGC > CP009273/2681224‑2681353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |