Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,901,251	C→A	G17V (GGT→GTT)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,901,251	0	C	A	97.7%	137.9 / ‑4.0	43	G17V (GGT→GTT)	eno	enolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (18/24);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAG  >  CP009273/2901186‑2901315                                                                  |                                                                 aCGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACa                                                                >  1:3983980/1‑68 (MQ=255)   ggAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACg                                                              >  1:4576850/1‑68 (MQ=255)      gcagcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGa                                                           >  1:4730172/1‑68 (MQ=255)       cagcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAg                                                          >  1:3912735/1‑68 (MQ=255)        agcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGt                                                         <  1:5038540/68‑1 (MQ=255)        agcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGt                                                         <  1:3320399/68‑1 (MQ=255)        agcTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGt                                                         <  1:2743250/68‑1 (MQ=255)        agcTGCCATACCGACAAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGt                                                         <  1:704054/68‑1 (MQ=255)           tGCCAGACCGACGAAACCACCCTCCAGATCTACTTCGGCTTCAACAGTCGGGATAACACGTGAGTCga                                                      >  1:4090674/1‑68 (MQ=255)            gCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCgat                                                     <  1:247738/68‑1 (MQ=255)             ccATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCgatg                                                    >  1:1702787/1‑68 (MQ=255)                tACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAtt                                                 <  1:997214/68‑1 (MQ=255)                 aCCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAttt                                                >  1:3812543/1‑68 (MQ=255)                 aCCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGAatt                                                >  1:5213753/1‑68 (MQ=255)                       gaAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGAc                                          <  1:3710476/68‑1 (MQ=255)                        aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGAcc                                         <  1:2419695/68‑1 (MQ=255)                           ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCTATGATTTCACGACCgat                                      >  1:4125332/1‑68 (MQ=255)                            caccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCgatg                                     >  1:4823040/1‑68 (MQ=255)                               ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGAtt                                  <  1:5224705/68‑1 (MQ=255)                               ccTCCACATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGAtt                                  <  1:2480314/68‑1 (MQ=255)                                     gATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACg                            >  1:1752733/1‑68 (MQ=255)                                     gATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACg                            >  1:3775672/1‑68 (MQ=255)                                     gATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACg                            <  1:1470311/68‑1 (MQ=255)                                     gATGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACg                            >  1:911458/1‑68 (MQ=255)                                       tgaaCTTCGGCTCCAACAGTCGGGTTAACACGGGAGGCGATGATTTCACGACCGATGATTTTTACGAt                          <  1:873630/65‑1 (MQ=255)                                       tctACTTCGCCTACAACAGACGCGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                          <  1:4937740/66‑1 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                          <  1:4456276/68‑1 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                          >  1:2773283/1‑68 (MQ=255)                                       tGTACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAt                          >  1:4850886/1‑68 (MQ=255)                                         tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                        >  1:3920190/1‑68 (MQ=255)                                         tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                        >  1:2268899/1‑68 (MQ=255)                                         tACTTCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGAttt                        >  1:1383505/1‑68 (MQ=255)                                             tCGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGa                    <  1:1868395/68‑1 (MQ=255)                                              cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                   <  1:1053918/68‑1 (MQ=255)                                              cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                   <  1:141821/68‑1 (MQ=255)                                              cGGCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                   <  1:5024325/68‑1 (MQ=255)                                              cGGCATCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGAc                   <  1:3869022/68‑1 (MQ=255)                                                gCTTCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACAt                 >  1:276172/1‑68 (MQ=255)                                                   tCAACAGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAg              <  1:4408057/68‑1 (MQ=255)                                                    cAACATTCGGGGTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTCGACATTAgg             <  1:1339123/68‑1 (MQ=255)                                                        aGTCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGtttt         <  1:356829/68‑1 (MQ=255)                                                          tCGGGTTAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTcc       <  1:420040/68‑1 (MQ=255)                                                               ttAACACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAg  <  1:5994003/68‑1 (MQ=255)                                                                  |                                                                 ACGGAGCAGCTGCCATACCGACGAAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAG  >  CP009273/2901186‑2901315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑