| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | Exported | 901 | Δ5 bp | intergenic (‑109/‑106) | aadA ← / → fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 901 | 0 | C | . | 92.1% | 98.8 / 8.4 | 38 | intergenic (‑109/‑110) | aadA/fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base . (23/12); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | Exported | 902 | 0 | A | . | 89.5% | 95.4 / 13.8 | 38 | intergenic (‑110/‑109) | aadA/fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base . (21/13); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01 | |||||||||||
| * | Exported | 903 | 0 | A | . | 92.1% | 100.0 / 8.8 | 38 | intergenic (‑111/‑108) | aadA/fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (22/13); total (24/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01 | |||||||||||
| * | Exported | 904 | 0 | T | . | 89.7% | 93.6 / 13.2 | 39 | intergenic (‑112/‑107) | aadA/fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base . (22/13); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | Exported | 905 | 0 | T | . | 89.7% | 82.9 / 12.5 | 39 | intergenic (‑113/‑106) | aadA/fdh | aminoglycoside adenylyltransferase (Murphy, 1985)/formate dehydrogenase from Pseudomonas sp. 101 with an N‑terminal his‑tag |
| Reads supporting (aligned to +/- strand): ref base T (2/2); new base . (23/12); total (25/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATCTCGAGACCTATTGACAATTAAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGACCGAATTCACTAGTTAATAGAA > Exported/836‑969 ||||| tGTCTCATGAGCGGATTCATATTTGTATGTATTTAGAAAAATAATCAATTCTGGAGACCTATTGaaaa > 1:5252030/1‑68 (MQ=17)tGTCTCATGAGCGGATACAGATTTGAATCTATTTAGAAAAATTAACAAATCTCGAGTCATATGGaaaa > 1:1052266/1‑68 (MQ=14) gTCTCATGAGCGGATACACATTTGAATGTATTTAGAAAAAAAACCAAATCTCGAGCCCTATTGaaaag < 1:1406549/68‑2 (MQ=17) tctcATGAGAGGATACATAATTGAAAGTAATTAGATAAATAAACAAATCTCTTGACCTATTGaaaagg > 1:5626833/1‑66 (MQ=9) ctcATGAGCGGATACATATATGAATGTATTTAGAAAAATTAACAAATCTCGAGTCCTATTGaaaaggc > 1:4942785/1‑65 (MQ=17) tACATATTTCAATGTATTTAGAAAAATAGTCAAATCTCGAGACCTATTG‑C‑‑‑‑AAAGGCTAAAATGCtata < 1:1003380/68‑1 (MQ=12) cATATTTGATTGTATTTAGAAAAATAATCAAATCTCGAGACCAATTGA‑‑‑‑‑AAAGGCTAATATGCTATAAt < 1:5298457/68‑1 (MQ=12) tatTTGAATGTATTTAGAATTATAAACAAATCTCGAGCCCTATTGA‑AAT‑‑‑‑GGCTAAAATGCTATAATTc > 1:1833524/1‑68 (MQ=16) atTTGACTGTATTTAGAAAAACAAAGAACACACGCGACGTATTGA‑‑‑‑‑AAAGGCTAAAATGCTATAATTcc < 1:1005372/68‑1 (MQ=9) atTTGAATGTATTTAGGAAAATAAAAAAATCTCGAGGCCTGTTGA‑‑‑‑‑AAAGGGTAAAATGGTATAATTcc > 1:4093779/1‑68 (MQ=9) atTTGAATGTATTTAGAAAGATAAACAAATCTCGAAACGAATTGA‑‑‑‑‑AAAGGCTAAGATGCTATAATTcc > 1:1005114/1‑68 (MQ=9) atTTGAATGTATTAAGAAAAATAAACAAATCACGAGCCCTATTGA‑‑‑‑‑AAAGCCTAAAATGCTATAATTcc > 1:3705591/1‑68 (MQ=12) ttGAATGTATTTAGAACAATAATCAAATCTCGAGTCCTATTGA‑‑‑‑TAACGG‑TATAATGCTATAATTCCAc < 1:5128887/68‑1 (MQ=12) tcaaTGTATTTAGATAAATAAACAATTCTCGAGCCCTATTGA‑‑‑‑‑AAAGGCTAATATGCTATAATTCCACt > 1:4767353/3‑68 (MQ=16) tGAATGTATTTAGAAAAATTAACAAATCTCGAGACCTATT‑‑CA‑‑‑AAAGGCTAAAATGCGATAATTCCACt > 1:2983777/1‑68 (MQ=16) tGAATGTATATAGAAAAATAAACAAATCTAGAGCCCTATTGA‑‑‑‑‑AAAGGCTAAAATCCTATAATTacaca > 1:3377068/1‑67 (MQ=12) caggTATTTAGAAAAATAAACATTTCTCGAGACCTATTGA‑‑‑‑‑AAATGATATAATGCTATAATTCCACTca < 1:4194043/65‑1 (MQ=12) aaTGTATTTCTAAAAACTAACAACTCTTGAGTCCTATTGA‑‑‑‑‑AAAGGCTAAAATGCTATAATTCCACTca < 1:4094931/68‑1 (MQ=255) aaTGTATTTAGAAAAATAATCACATCTCGAGACGTATTGA‑‑‑‑‑AACGGCTAAATTGCTAGAATTCCACTca > 1:2280266/1‑68 (MQ=12) aaTGGATTTAGAAAAATCAACAAATCTCGAGACCTATGGA‑‑‑‑‑AAAGGCTAAAATGCTATCATTCCACTca < 1:1427354/68‑1 (MQ=19) aTGTATTTAGAAAAATAATCAAATCTCGAGTCCTATTGA‑AAT‑‑‑‑GGCTAAAATGCTTTAATTCCACTcag > 1:4035973/1‑68 (MQ=16) aTGTATTTAGAAAAATAATCAAATCTCGAGACGTTTTGA‑‑‑‑‑AAAGGCTAATTTGCTATAATTCCACTcag > 1:265539/1‑68 (MQ=16) tGTATTTAGAAACATAAACAAATCTCTAGACCTATTG‑‑AAT‑‑‑ATGCTAAAATGATATAATTCCACTcagc < 1:4881612/68‑1 (MQ=16) tGTATTTAGAAAAATTAACAATTCTCGAGACCGATTGA‑‑‑‑‑AAAGGCTAAATTGCTATAATTCCACTcagc > 1:1601967/1‑68 (MQ=255) tGTATTTAGAAAAATATACAAATCTCGAGACGGATTGA‑‑‑‑‑AAAGGCTAAATTGCTATAATTCCCCTcagc > 1:4666682/1‑68 (MQ=16) tGTATTTAGAAAAATAAACTAATCTCGAGACCAATTGA‑‑‑‑‑AAAGGCTAAAATGCAAAAATTCCACTcagc > 1:5626150/1‑68 (MQ=16) ggTATTTAGAAAAATAAACAAAGCTCGAGACCTATTGA‑‑‑‑‑AACGGCTAAAATGCTATAATTCGACTccgc > 1:725733/2‑68 (MQ=16) gTATTTATAAAAATAAACGAATCTCGAGACCTATTGA‑‑‑‑‑AAGGGCTAAAATGCTATGATTCCACTcagca > 1:875142/1‑68 (MQ=255) gTATTTAGAAAAATAGACAAATCTCGAGACCTAGTGA‑‑‑‑‑AAATGCGAAAATGCGATAATTCCCCTcagcc > 1:1503799/1‑67 (MQ=12) tacttAGAAAAATATACAAATCACGAGACCTATTGA‑‑‑‑‑ACAGGCTAAAACGCTAAAATTCCACTcagcag < 1:5505911/65‑1 (MQ=255) aTTTATAAAAATAAACAAATATCGAGAACTATTGA‑‑‑‑‑AAAGGCTAAAATTCTATAATTCAACTCAGCAgg < 1:1619046/68‑1 (MQ=255) aTTTAGAAATATAAACACATCTCGAGACCTAGTGA‑‑‑‑‑AAAGGCTAAAATGCTACAATTCCACTCAGCAgg < 1:4094159/68‑1 (MQ=255) tttAGAATAATAAAGAAATCTCGAGACCTTTTG‑‑‑‑‑TAATGGCTATATTGCTATAATTCCACTCTGCAGGa < 1:1253283/68‑1 (MQ=255) ttAGAAAATTAAACATATCTCGAGACCTATTGA‑‑‑‑T‑AAGGCTAAAATGCTATATTTCGACTCAGCAGGAc > 1:3830645/1‑68 (MQ=255) ttAGAAAAATATACAAATCTCGAGAGCTATTGA‑‑‑‑‑AAAGGGTAAAATGCTATAATTGCACTCAGCAGGAc > 1:3733341/1‑68 (MQ=255) ttAGAAAAATAAACTAATCTCGAGACCTTTTGA‑‑‑‑‑AAAGGCTAAAATGCTTTAATTCCACTCAGCAGGAc > 1:5573428/1‑68 (MQ=255) tAGCAAAATAAACAAATCTCGAGAACTATTG‑C‑‑‑‑AAAGGCTAAAATCCTATAATTCCTCTCAGCAGGGCg < 1:1099612/68‑1 (MQ=255) gAAAAATAAACAAAACTCGAGACCTATTGA‑‑‑‑‑AAAGTCTAAAATGCTATAAATCCACTCAGCAGGACGCa < 1:5090336/68‑1 (MQ=255) gAAAAATAAAAAAATCTCGAGAACTATTGA‑‑‑‑‑AAAGGCTTAAATGCTATAATTCCTCTCAGCAGGACGCa > 1:4255710/1‑68 (MQ=255) aaaaTATACAAATCTAGAGACCTATTGA‑‑‑‑‑AAAGGCTAAATTGCTATAATTCGACTCAGAGggacggacg > 1:3715129/1‑67 (MQ=255) aaaaTAAACAAATCTCGAGACCTAGTGA‑‑‑‑‑AGAGGCTAGAGTGCTATAATTCGACTCAGGGGGACGCACt > 1:4095352/1‑68 (MQ=255) aTAAACAAATCTCGAGTCCTATTGA‑‑‑‑‑AAAGGCTAAAAGGCTATAATTCCACTCAGCAGGCCGCGCTGAc > 1:4643498/1‑68 (MQ=255) aCAAATCTCGAGACCTAATGA‑‑‑‑‑AAAGGCTACAATGCTATAAGTCCAGTCACCCGGACGCACTGACCGaa > 1:917516/1‑68 (MQ=255) cctaTTGAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGACCGAATTCACTAGTTAATAg > 1:2567300/4‑68 (MQ=255) ttgtAAAGGCTAAAATGCTATAATTCCACTCAGCATGACGCACTGACCGAATTCACTAGTTAATAGaa < 1:5072289/65‑1 (MQ=255) ttgtAAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGTCCGAATTCACTAGTTAATAGaa < 1:3258927/65‑1 (MQ=255) ttgtAAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGACCGAATTCACTAGTTAATAGaa < 1:1744457/65‑1 (MQ=255) ttgtAAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGACCGAATTCACTAGTTAATAGaa < 1:1622193/65‑1 (MQ=255) ttgtAAAGGCTAAAATGCTATAAATCCACTCAGCAGGACGCACTGACCGATTTCACTAGTTAATAGaa < 1:2078562/65‑1 (MQ=21) ||||| TGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATCTCGAGACCTATTGACAATTAAAGGCTAAAATGCTATAATTCCACTCAGCAGGACGCACTGACCGAATTCACTAGTTAATAGAA > Exported/836‑969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |