Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	3,466,500	G→A	T125I (ACC→ATC)	fusA ←	protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,466,500	0	G	A	100.0%	142.9 / NA	44	T125I (ACC→ATC)	fusA	protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

GCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACG  >  CP009273/3466436‑3466564                                                                 |                                                                 gCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGCCGCCATACGAttt                                                               >  1:1695367/1‑68 (MQ=255) gCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGAttt                                                               >  1:1211605/1‑68 (MQ=255) gCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGAttt                                                               <  1:2237959/68‑1 (MQ=255)   ggTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCa                                                             >  1:5296626/1‑68 (MQ=255)   ggTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCa                                                             >  1:3695074/1‑68 (MQ=255)   ggTCCATTTTGTTAACGAACCCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCa                                                             >  1:284902/1‑68 (MQ=255)     tCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGa                                                           <  1:372608/68‑1 (MQ=255)       cATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACt                                                         <  1:834443/68‑1 (MQ=255)       cATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACt                                                         <  1:2108361/68‑1 (MQ=255)             gTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCt                                                   >  1:3886088/1‑68 (MQ=255)                   gaacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAacac                                             <  1:2250380/68‑1 (MQ=255)                   gaacgCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAacac                                             <  1:449318/68‑1 (MQ=255)                   gaaccCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAacac                                             <  1:2413411/68‑1 (MQ=255)                       gCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACaccacc                                         >  1:2625114/1‑68 (MQ=255)                           tGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACt                                     <  1:251978/68‑1 (MQ=255)                           tGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACt                                     <  1:872136/68‑1 (MQ=255)                           tGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACt                                     <  1:4939693/68‑1 (MQ=255)                                ggAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCa                                <  1:4222884/68‑1 (MQ=255)                                ggAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCa                                <  1:5629767/68‑1 (MQ=255)                                ggAACTTTATATTTGTTAGCCTGACGCCATACGATTTGAGACTGCGGCTGAACACCACCAACTGCGCa                                <  1:724561/68‑1 (MQ=255)                                 gAACTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAg                               <  1:4422498/68‑1 (MQ=255)                                 gAACTTTATATTTGATTGCCTGACGCCATACGATTTCAGACTGCGTCTGAAAACCACCAACTGCGCAg                               <  1:4953869/68‑1 (MQ=255)                                  aaCTTTATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGt                              <  1:3322244/68‑1 (MQ=255)                                      ttATATTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAAc                          <  1:4090037/68‑1 (MQ=255)                                        atatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa                        <  1:3362584/68‑1 (MQ=255)                                        atatTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCa                        >  1:1680273/1‑68 (MQ=255)                                          atTTGTTTGCCTGACGCCATACGATTTCCGACTGCGGCTGAACACTACCAACTGCGCAGTAAACCAtt                      <  1:902173/68‑1 (MQ=255)                                          atTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt                      >  1:1629565/1‑68 (MQ=255)                                          atTTGTTTGCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAtt                      >  1:4758074/1‑68 (MQ=255)                                           tttgtttgCCTGACGCCATACGATATCAGACTGCGGCTGAACACCACCAACTGCGCAGGAAACCATTa                     >  1:2565628/1‑68 (MQ=255)                                             tgtttgCCTGCCGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                   >  1:1254140/1‑68 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGCCCACCACCAACTGCGCAGTAAACCATTAcc                   >  1:3304677/1‑68 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGCACACCACCAACTGCGCTGTAAACCATTAcc                   >  1:3525622/1‑68 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                   >  1:4598744/1‑68 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                   <  1:3857058/68‑1 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                   >  1:204511/1‑68 (MQ=255)                                             tgtttgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTAcc                   >  1:1962557/1‑68 (MQ=255)                                                 tgCCTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCAc               <  1:4151120/68‑1 (MQ=255)                                                    cTGACGCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCAt            >  1:1698134/1‑68 (MQ=255)                                                      gACGCCATACCATTTCAGACTGCGGCTGAACACCACTAACTGCGCAGTAAACCATTACCGCACCATCg          <  1:1020014/68‑1 (MQ=255)                                                         gCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCgaga       >  1:990194/1‑68 (MQ=255)                                                         gCCATACGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCAGTACCGCACCATCgaga       >  1:472620/1‑68 (MQ=255)                                                              aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg  <  1:3238138/68‑1 (MQ=255)                                                              aCGATTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACg  >  1:2759510/1‑68 (MQ=255)                                                                 |                                                                 GCGGTCCATTTTGTTAACGAACGCAATGCGCGGAACTTTATATTTGTTTGCCTGACGCCATACGGTTTCAGACTGCGGCTGAACACCACCAACTGCGCAGTAAACCATTACCGCACCATCGAGAACACG  >  CP009273/3466436‑3466564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑