Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,275,144	C→T	intergenic (‑3/+197)	yjcH ← / ← acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,275,144	0	C	T	100.0%	279.1 / NA	82	intergenic (‑3/+197)	yjcH/acs	DUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/51);  total (31/51)

CGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAC  >  CP009273/4275084‑4275207                                                             |                                                                cGACTAACTCCCTTAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                          <  1:501652/68‑1 (MQ=255) cGACTAACTCCCTGAAATGCTCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                          <  1:5449536/68‑1 (MQ=255) cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                          <  1:1832752/68‑1 (MQ=255) cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATtct                                                          <  1:4944906/68‑1 (MQ=255)      aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCaga                                                     <  1:442079/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:568834/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:201194/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:1988818/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:1495828/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:3378670/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:3553234/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAAAAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:2021353/68‑1 (MQ=255)       aCTCCCTGAAATGCGCATTGTCTTCTATCCGCCGATAAATAGTGCCATTCATCATAGATTCTCCagag                                                    <  1:3877700/68‑1 (MQ=255)       aCTCCCTGAAATGCGCACTGTCTTCTATCCGCTGATAAATAGTTCCATTCATCATAGATTCTCCagag                                                    <  1:993319/68‑1 (MQ=255)         tCCCTGAACTGCGCATTGTGTTCTATCCGCTGATAAATAGTGCCATTCATCTTAGATTCTCCagagca                                                  <  1:1263939/68‑3 (MQ=255)         tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                  >  1:1657574/1‑68 (MQ=255)         tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                  >  1:1572656/1‑68 (MQ=255)         tCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                  <  1:1300740/68‑1 (MQ=255)         tCCCTGAAAAGCGCATTGGCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGt                                                  <  1:5561320/68‑1 (MQ=255)           ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTaa                                                <  1:5319973/68‑1 (MQ=255)           ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTaa                                                <  1:5481917/68‑1 (MQ=255)            cTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAAt                                               <  1:3256027/68‑1 (MQ=255)             tGAAATGCGCATTGTCTTCTATCCGCTAATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATg                                              <  1:51505/68‑1 (MQ=255)              gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGt                                             >  1:4311318/1‑68 (MQ=255)              gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGt                                             >  1:270659/1‑68 (MQ=255)               aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTa                                            >  1:4374725/1‑68 (MQ=255)                 aTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAgg                                          >  1:3289202/1‑68 (MQ=255)                      cATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTg                                     >  1:1166972/1‑68 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATTGTGCCATTCATCTTCGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:711407/68‑1 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:558469/68‑1 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:1736705/68‑1 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:5561335/68‑1 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:1197710/68‑1 (MQ=255)                       aTTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGt                                    <  1:1841192/68‑1 (MQ=255)                        ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtt                                   <  1:1410239/68‑1 (MQ=255)                        ttGTCTTCTATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGtt                                   <  1:4995775/68‑1 (MQ=255)                         tggcttctATCCGCTGATAATTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGttt                                  <  1:3772859/65‑1 (MQ=255)                               ctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                            <  1:3580557/68‑1 (MQ=255)                               ctATCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                            <  1:4471230/68‑1 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:1613397/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:4823152/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:13202/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:3468324/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:2991073/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:4301845/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                          >  1:2680315/1‑68 (MQ=255)                                 aTCCGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGCGATTGTGTAAATTccc                          >  1:4310098/1‑68 (MQ=255)                                   ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                        >  1:4129414/1‑68 (MQ=255)                                   ccGCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                        >  1:705275/1‑68 (MQ=255)                                   ccGCTGATAAATAGTGCCATTCATAATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGc                        >  1:4062174/1‑68 (MQ=255)                                     gCTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc                      <  1:1802912/68‑1 (MQ=255)                                     gCTGATAAATAGTGCCATTCATCATAGATTCTACAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTc                      <  1:2163830/68‑1 (MQ=255)                                      cTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                     <  1:3563062/68‑1 (MQ=255)                                      cTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                     <  1:5228195/68‑1 (MQ=255)                                      cTGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTcc                     <  1:4066632/68‑1 (MQ=255)                                       tGATATATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:1824369/68‑1 (MQ=255)                                       tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:3229781/68‑1 (MQ=255)                                       tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:1784554/68‑1 (MQ=255)                                       tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:169199/68‑1 (MQ=255)                                       tGATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:3446401/68‑1 (MQ=255)                                       ggATAAATAGTGCCATTCATCATAGATTCTCCAGCGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                    <  1:2186006/67‑1 (MQ=255)                                        gATAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                   <  1:1194195/68‑1 (MQ=255)                                         aTAAATAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                  <  1:222228/68‑1 (MQ=255)                                             aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg              >  1:525269/1‑68 (MQ=255)                                             aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg              >  1:4694369/1‑68 (MQ=255)                                             aTAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg              >  1:3350776/1‑68 (MQ=255)                                              tAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATggg             <  1:5595377/68‑1 (MQ=255)                                              tAGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATggg             <  1:2763510/68‑1 (MQ=255)                                               aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga            >  1:1820007/1‑68 (MQ=255)                                               aGTGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGga            >  1:3168424/1‑68 (MQ=255)                                                 tGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgaga          >  1:3498032/1‑68 (MQ=255)                                                 tGCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgaga          >  1:3168667/1‑68 (MQ=255)                                                  ggcATTCATCATAGATTCTCCAGAGGTAATGAAGTGATTGTTTTAATTCCAGCTCCCTTATGgaagaa         >  1:1527018/3‑68 (MQ=255)                                                  gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa         >  1:3026673/1‑68 (MQ=255)                                                  gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa         >  1:5080690/1‑68 (MQ=255)                                                  gCCATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGaa         >  1:1053901/1‑68 (MQ=255)                                                    cATTCATCATAGATTCTCCAGAGGTAATGTTGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg       <  1:1184877/68‑1 (MQ=255)                                                    cATTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgg       <  1:1496293/68‑1 (MQ=255)                                                     aTTCATCATAGCTTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAgtt      <  1:5251967/68‑3 (MQ=255)                                                     aTTCATCATAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGt      <  1:428281/68‑1 (MQ=255)                                                       tcatcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa    >  1:4289900/1‑68 (MQ=255)                                                         atcaTAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAc  >  1:947949/1‑68 (MQ=255)                                                             |                                                                CGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAAC  >  CP009273/4275084‑4275207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑